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MudPIT analysis of alkaline tolerance by Listeria monocytogenes strains recovered as persistent food factory contaminants

机译：作为食品工厂的持久性污染物回收的单核细胞增生李斯特菌菌株的耐性MudPIT分析

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摘要

Alkaline solutions are used to clean food production environments but the role of alkaline resistance in persistent food factory contamination by Listeria monocytogenes is unknown. We used shotgun proteo-mics to characterise alkaline adapted L. monocytogenes recovered as persistent and transient food factory contaminants. Three unrelated strains were studied including two persistent and a transient food factory contaminant determined using multilocus sequence typing (MLST). The strains were adapted to growth at pH 8.5 and harvested in exponential phase. Protein extracts were analysed using multidimensional protein identification technology (MudPIT) and protein abundance compared by spectra counting. The strains elicited core responses to alkaline growth including modulation of intracellular pH, stabilisation of cellular processes and reduced cell-division, independent to lineage, MLST or whether the strains were transient or persistent contaminants. Alkaline adaptation by all strains corresponded to that expected in stringent-response induced cells, with protein expression supporting metabolic shifts concordant with elevated alarmone production and indicating that the alkaline-stringent response results from energy rather than nutrient limitation. We believe this is the first report describing induction of a stringent response in different L. monocytogenes strains by alkaline pH under non-limiting growth conditions. The work emphasises the need for early intervention to avoid persistent food factory contamination by L monocytogenes.
机译：碱性溶液用于清洁食品生产环境，但尚不知道碱性抗性在单核细胞增生性李斯特菌污染食品工厂的持久性中的作用。我们使用shot弹枪蛋白质组学来鉴定碱性和适应性单核细胞增生李斯特菌，它们被作为持久性和暂时性食品工厂污染物而回收。研究了三种不相关的菌株，其中包括使用多基因座序列分型（MLST）确定的两个持久性食品和暂时性食品工厂污染物。使菌株适应于在pH 8.5下生长并在指数期收获。使用多维蛋白质鉴定技术（MudPIT）分析蛋白质提取物，并通过光谱计数比较蛋白质丰度。菌株引起对碱性生长的核心反应，包括调节细胞内pH，稳定细胞过程和减少细胞分裂，而与谱系，MLST或菌株是暂时性还是持久性污染物无关。所有菌株的碱性适应性均与严格应答诱导的细胞中预期的适应性一致，蛋白质表达支持代谢变化与警报素产生的增加相一致，并表明碱性严格应答源自能量而非营养限制。我们认为这是描述非碱性生长条件下碱性pH诱导不同单核细胞增生李斯特氏菌菌株严格反应的第一份报告。这项工作强调需要及早干预，以避免食品工厂持续受到单核细胞增生李斯特氏菌的污染。

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  • 来源
    《Food microbiology》 |2012年第1期|p.187-196|共10页
  • 作者

    Rolf E. Nilsson; Roger Latham; Lyndal Mellefont; Tom Ross; John P. Bowman;

  • 作者单位

    Food Safety Centre, Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart 7001, Tasmania, Australia;

    Food Safety Centre, Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart 7001, Tasmania, Australia;

    Food Safety Centre, Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart 7001, Tasmania, Australia;

    Food Safety Centre, Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart 7001, Tasmania, Australia;

    Food Safety Centre, Tasmanian Institute of Agricultural Research, School of Agricultural Science, University of Tasmania, Private Bag 54, Hobart 7001, Tasmania, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    MudPIT; alkaline; persistent; L.l monocytogenes; stringent response;

    机译：MudPIT;碱性持久单核细胞增生李斯特菌;严格的回应;

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