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首页> 外文期刊>Food microbiology >Survival of Lactobadllus rhamnosus strains inoculated in cheese matrix during simulated human digestion
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Survival of Lactobadllus rhamnosus strains inoculated in cheese matrix during simulated human digestion

机译：模拟人体消化过程中奶酪基质中接种的鼠李糖乳杆菌的存活

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摘要

Survival of probiotic bacteria during transit through the gastrointestinal (GI) tract is influenced by a number of environmental variables including stomach acidity, bile salts, digestive enzymes and food matrix. This study assessed survival of seven selected Lactobadllus rhamnosus strains delivered within a model cheese system to the human upper Gl tract using a dynamic gastric model (DGM). Good survival rates for all tested strains were recorded during both simulated gastric and duodenal digestion. Strains H12, H25 and N24 demonstrated higher survival capacities during gastric digestion than L rhamnosus GG strain used as control, with H12 and N24 continuing to grow during duodenal digestion. Strains L rhamnosus F17, N24 and R61 showed adhesion properties to both HT-29 and Caco-2 cells. The ability to attach to the cheese matrix during digestion was confirmed by scanning electron microscopy, also indicating production of extracellular polysaccharides as a response to acid stress.
机译：益生菌在通过胃肠道的过程中的存活受到许多环境变量的影响，包括胃酸度，胆汁盐，消化酶和食物基质。这项研究评估了使用动态胃部模型（DGM）在模型干酪系统中递送至人上胃肠道的七种选定的鼠李糖乳杆菌菌株的存活率。在模拟胃和十二指肠消化过程中，记录了所有测试菌株的良好存活率。 H12，H25和N24菌株在胃消化过程中的存活能力要高于鼠李糖鼠GG菌株，在十二指肠消化过程中H12和N24继续生长。鼠李糖鼠李糖F17，N24和R61对HT-29和Caco-2细胞均表现出粘附特性。消化过程中附着在干酪基质上的能力已通过扫描电子显微镜得到证实，也表明细胞外多糖的产生是对酸胁迫的反应。

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  • 来源
    《Food microbiology》 |2012年第1期|p.57-63|共7页
  • 作者

    Iole Pitino; Cinzia L. Randazzo; Kathryn L. Cross; Mary L Parker; Carlo Bisignano; Martin S.J. Wickham; Giuseppina Mandalari; Cinzia Caggia;

  • 作者单位

    DOFATA, Dipartimento di Orto Flow Arboricoltura e Tecnologie Agro-alimentari, University of Catania, via Santa Sofia 98, 95123 Catania. Italy,Model Gut Platform, Institute of Food Research, Norwich NR4 7UA, UK;

    DOFATA, Dipartimento di Orto Flow Arboricoltura e Tecnologie Agro-alimentari, University of Catania, via Santa Sofia 98, 95123 Catania. Italy;

    Imaging Partnership, Institute of Food Research, Norwich NR4 7UA, UK;

    Imaging Partnership, Institute of Food Research, Norwich NR4 7UA, UK;

    Pharmaco-Biological Department, University of Messina, Viale Annunziata, 98168 Messina, Italy;

    Model Gut Platform, Institute of Food Research, Norwich NR4 7UA, UK;

    Model Gut Platform, Institute of Food Research, Norwich NR4 7UA, UK,Pharmaco-Biological Department, University of Messina, Viale Annunziata, 98168 Messina, Italy;

    DOFATA, Dipartimento di Orto Flow Arboricoltura e Tecnologie Agro-alimentari, University of Catania, via Santa Sofia 98, 95123 Catania. Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    lactobadllus rhamnosus; dynamic gastric model; survival; cheese;

    机译：鼠李糖乳杆菌动态胃模型生存起司;

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