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首页> 外文期刊>Food microbiology >Occurrence of non-0157 shiga toxin-producing Escherichia coli in ready-to-eat food from supermarkets in Argentina
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Occurrence of non-0157 shiga toxin-producing Escherichia coli in ready-to-eat food from supermarkets in Argentina

机译：阿根廷超市的即食食品中发生的非0157产志贺毒素的大肠杆菌的发生

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摘要

Between June 2000 and December 2001, 500 food samples were collected from supermarkets and shops selling ready-to-eat food in Rosario, Argentina, and examined for Escherichia coli. Forty-nine E. coli isolates from food samples were further characterized for virulence genes by multiplex polymerase chain reaction (PCR) targeting the stx1, stx2, stx2e, eaeA, CNF1, CNF2, Einv, LTI, STI, and STII genes in four groups. Out of 49 E. coli isolates screened by multiplex PCR, only 10 possessed Shiga toxin genes, stx1and stx2 genes and none possessed the other genes. The Shiga toxin positive E. coli strains (STEC) were isolated from soft, cottage cheeses, chicken with sauce and vegetables mayonase. These E. coli isolates were serogrouped and belonged to O18 (two strains), O8, O57w, O79, O44, and O128; three strains were untypeable. Pulsed-field gel electrophoresis (PFGE) with XbaI generated a unique profile for each, having 10-15 bands ranging from 50 to 500 kb, except that strain ARG 20 generated small bands and was partly degraded. These strains are potential foodborne pathogens and their presence in ready-to-eat food illustrates the need to keep a careful watch for the source of pathogens and then develop methods to control them.
机译：在2000年6月至2001年12月之间，从在阿根廷罗萨里奥（Rosario）出售即食食品的超市和商店收集了500个食品样本，并检查了大肠杆菌。通过针对四组中的stx1，stx2，stx2e，eaeA，CNF1，CNF2，Einv，LTI，STI和STII基因的多重聚合酶链反应（PCR），进一步对食品样本中的49株大肠杆菌进行了毒力基因鉴定。 。通过多重PCR筛选的49株大肠杆菌中，只有10株具有志贺毒素基因，stx1和stx2基因，而没有其他基因。志贺毒素阳性大肠杆菌菌株（STEC）从柔软的干酪，酱鸡和蔬菜蛋黄酱中分离出来。这些大肠杆菌分离物按血清分组，分别属于O18（两个菌株），O8，O57w，O79，O44和O128。三个菌株是无法分型的。带有XbaI的脉冲场凝胶电泳（PFGE）各自产生了独特的图谱，具有10-15条从50到500 kb的条带，除了ARG 20菌株产生了小条带并且部分降解了。这些菌株是潜在的食源性病原体，它们在即食食品中的存在说明需要仔细观察病原体的来源，然后开发控制它们的方法。

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