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Quantification of marker compounds in Cirsium setidens Nakai by HPLC-DAD

机译：HPLC-DAD定量测定中kai碱中的标记物

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摘要

An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R 2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 μg/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 μg/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06–105.81% with an RSD value of 1.87–3.50%. In conclusion, the results of validation showed that the proposed HPLC method was useful for the quantification of the two marker compounds in C. setidens.
机译：开发了一种高效液相色谱-二极管阵列检测器（HPLC-DAD）的有效方法，用于同时定量测定韩国野生药草rs中的两种标记化合物果胶素和果胶素。用梯度系统在C18色谱柱上分离了C. setidens的两种标记物。两种标准化合物的校准曲线均显示出良好的线性（R 2> 0.9992）。果胶素和果胶素的检出限（LOD）分别为0.52和0.41μg/ mL，而定量限（LOQ）分别为1.57和1.25μg/ mL。日内和日间实验数据的相对标准偏差（RSD）分别小于1.65和2.78％。回收率测试的结果为98.06–105.81％，RSD值为1.87–3.50％。总之，验证的结果表明，所提出的HPLC方法可用于定量测定棉铃虫中的两种标记物。

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