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Simultaneous saccharification and fermentation of ethanol from potato waste by co-cultures of Aspergillus niger and Saccharomyces cerevisiae in biofilm reactors

机译:黑膜曲霉和酿酒酵母在生物膜反应器中的共培养,同时马铃薯马铃薯废液中的乙醇糖化和发酵

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摘要

Novel approaches for bioethanol production from industrial wastes have gained attention due to not only maximize the ethanol production productivity, but also reduce the production cost. Part of the production cost includes cost of the enzymes needed for saccharification step during the starch hydrolyzation, which can be significant cost depending on the enzymes' performances. Therefore, in this study, simultaneous saccharification and fermentation (SSF) of ethanol by co-cultures of Aspergillus niger and Saccharomyces cerevisiae was assessed in a potato waste based medium by using biofilm reactors. The plastic composite supports (PCS) were studied for biofilm formation. Effects of temperature, pH, and aeration rates in biofilm reactors were evaluated by response surface methodology and the optimal conditions were found to be 35 degrees C, pH 5.8, and no aeration. The maximum ethanol concentration of 37.93 g/L was achieved at the end of 72 h fermentation, with a 0.41 g ethanol/g starch yield. Finally, biofilm formation of co-culture on PCS was also evaluated by scanning electron microscope. In conclusion, the results demonstrated that PCS can be utilized for SSF processes for ethanol production in biofilm reactors with co-cultures by using starchy industrial wastes. (C) 2017 Elsevier Ltd. All rights reserved.
机译:从工业废料生产生物乙醇的新方法不仅使乙醇生产效率最大化,而且降低了生产成本,因而受到关注。生产成本的一部分包括淀粉水解过程中糖化步骤所需的酶的成本,根据酶的性能,这可能是巨大的成本。因此,在这项研究中,通过使用生物膜反应器,在基于马铃薯废物的培养基中评估了黑曲霉和啤酒酵母共培养的同时乙醇糖化和发酵(SSF)。研究了塑料复合材料载体(PCS)的生物膜形成。通过响应面方法评估了生物膜反应器中温度,pH和通气速率的影响,发现最佳条件为35摄氏度,pH 5.8和无通气。发酵72小时结束时,最大乙醇浓度达到37.93 g / L,乙醇/克淀粉产量为0.41 g / L。最后,还通过扫描电子显微镜评估了在PCS上共培养的生物膜形成。总之,结果表明PCS可用于SSF工艺中,通过使用淀粉状工业废料,在与共培养物一起的生物膜反应器中生产乙醇。 (C)2017 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Fuel》 |2017年第15期|260-270|共11页
  • 作者单位

    Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA;

    Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA|Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biofilm; SSF; Co-culture; Bioethanol; Potato waste; Biofuel;

    机译:生物膜;SSF;共培养;生物乙醇;马铃薯废物;生物燃料;

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