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首页> 外文期刊>Fuel >Improved simultaneous saccharification and fermentation of bioethanol from industrial potato waste with co-cultures of Aspergillus niger and Saccharomyces cerevisiae by medium optimization
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Improved simultaneous saccharification and fermentation of bioethanol from industrial potato waste with co-cultures of Aspergillus niger and Saccharomyces cerevisiae by medium optimization

机译:通过培养基优化改善了工业马铃薯废液与黑曲霉和酿酒酵母共培养同时糖化和发酵的生物乙醇

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摘要

For economical production of bioethanol from industrial wastes, enzyme cost for saccharification is the significant cost for the overall process. Therefore, the fermentation medium optimization for simultaneous saccharification and fermentation (SSF) by using co-culture of Aspergillus niger and Saccharomyces cerevisiae for potato wastes was undertaken in this study. A medium consisted of waste potato mash, malt extract and FeSO4 center dot 7H(2)O were defined as the optimum medium for co-culture of A. niger and S. cerevisiae SSF. Statistical optimization of defined medium was conducted using central composite design. Optimization results suggested that optimum concentrations of industrial waste potato mash, malt extract, and FeSO4 center dot 7H(2)O were 92.37 g/L, 59.42 g/L and 0.159 g/L, respectively. Under the optimal medium, 35.19 g/L ethanol production and 31.36 U/ml glucoamylase activity giving a yield of 0.38 g ethanol/g starch and 0.34 U/g starch respectively, achieved at 30 degrees C and 120 h of fermentation. (C) 2016 Elsevier Ltd. All rights reserved.
机译:为了从工业废料经济地生产生物乙醇,糖化酶的成本是整个过程的重大成本。因此,在这项研究中,通过使用黑曲霉和酿酒酵母对马铃薯废料的共培养进行了同时糖化和发酵的发酵培养基的优化。由废马铃薯泥,麦芽提取物和FeSO4中心点7H(2)O组成的培养基被定义为A.niger和S.cerevisiae SSF共培养的最佳培养基。使用中央复合设计对定义培养基进行统计优化。优化结果表明,工业废土豆泥,麦芽提取物和FeSO4中心点7H(2)O的最佳浓度分别为92.37 g / L,59.42 g / L和0.159 g / L。在最佳培养基下,在30℃和120 h的发酵条件下,乙醇产量为35.19 g / L,葡糖淀粉酶活性为31.36 U / ml,分别得到0.38 g乙醇/ g淀粉和0.34 U / g淀粉。 (C)2016 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Fuel》 |2016年第1期|684-691|共8页
  • 作者单位

    Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA;

    Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA|Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Co-culture; Medium optimization; Ethanol; SSF; Industrial waste potato mash;

    机译:共培养;培养基优化;乙醇;SSF;工业废土豆泥;

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