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首页> 外文期刊>Genetic Resources and Crop Evolution >Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions
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Novel and ancient HMW glutenin genes from Aegilops tauschii and their phylogenetic positions

机译：tauschii的新的和古代的HMW谷蛋白基因及其系统发育位置。

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摘要

A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1Dx3.1t and 1Dy11*t were revealed and characterized from Aegilops tauschii Coss. subspecies tauschii accession AS60. SDS-PAGE band of 1Dx3.1t was between those of 1Dx2 and 1Dx3, while 1Dy11*t was between 1Dy11 and 1Dy12. The lengths of 1Dx3.1 t and 1Dy11* t were 2,514 bp and 1,968 bp, encoding 836 and 654 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions in Escherichia coli. Network analysis indicated that 1Dx3.1 t together with other five rare alleles only detected in Asia common wheat populations represented the ancestral sequences in Glu-D1 locus. Neighbor-joining tree analysis of previously cloned x-type and y-type alleles in the Glu-D1 locus supported the hypothesis that more than one Ae. tauschii genotypes were involved in the origin of hexaploid wheat and that different Ae. tauschii accessions contributed the D genome to common wheat and Ae. cylindrical Host, respectively. An Ae. tauschii accession with 1Dx3.1 t or a closely related allele probably have involved in the origin of common wheat. Since accession AS60 used in this study belonged to typical ssp. tauschii, present results suggested the possibility that ssp. tauschii was involved in the evolution of common wheat.
机译：从Taegchii Coss中发现并鉴定了一对新的高分子量谷蛋白亚基（HMW-GS）1Dx3.1t 和1Dy11 * t 。 tauschii亚种AS60。 1Dx3.1t 的SDS-PAGE带在1Dx2和1Dx3之间，而1Dy11 * t 在1Dy11和1Dy12之间。 1Dx3.1 t 和1Dy11 * t 的长度分别为2514 bp和1968 bp，分别编码836和654个氨基酸残基。通过在大肠杆菌中成功表达编码区，证实了它们的真实性。网络分析表明，仅在亚洲普通小麦群体中检测到的1Dx3.1 t 以及其他五个罕见等位基因代表了Glu-D1基因座中的祖先序列。 Glu-D1基因座中先前克隆的x型和y型等位基因的邻居加入树分析支持了一个以上Ae的假设。 tauschii基因型与六倍体小麦的起源和不同的Ae有关。 tauschii种质为普通小麦和Ae贡献了D基因组。圆柱形主机。阿姨1Dx3.1 t 的tauschii保藏号或一个密切相关的等位基因可能与普通小麦的起源有关。由于本研究中使用的登录号AS60属于典型的ssp。 tauschii，目前的结果表明，ssp的可能性。陶氏菌参与了普通小麦的进化。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2012年第8期|p.1649-1657|共9页
  • 作者

    Wen-Jie Chen; Xing Fan; Bo Zhang; Bao-Long Liu; Ze-Hong Yan; Lian-Quan Zhang; Zhong-Wei Yuan; You-Liang Zheng; Huai-Gang Zhang; Deng-Cai Liu;

  • 作者单位

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China;

    Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, Qinghai, People’s Republic of China;

    Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, Qinghai, People’s Republic of China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China;

    Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, People’s Republic of China;

    Triticeae Research Institute;

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  • 正文语种 eng
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  • 关键词

    Aegilops tauschii; Bacterial expression; HMW-GS; Phylogenetic analysis;

    机译：牛肝菌;细菌表达;HMW-GS;系统发育分析;

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