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Novel HMW glutenin genes from Aegilops tauschii and their unique structures

机译：牛肝菌新的HMW谷蛋白基因及其独特结构

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摘要

A pair of novel high-molecular-weight glutenin subunits (HMW-GS) 1DX5.3~t and lDyl2.1~(**t) were revealed and characterized from Ae. tauschii accession PI554324. SDS-PAGE band of 1Dx5.3~t was between those of 1Bx6 and 1Bx7, while lDyl2.1~(**t) with slightly faster migration rate than that of lDyl2. The lengths of Wx5.3' and lDyl2.1~(**t) were 2115 bp and 1986 bp, encoding 703 and 660 amino acid residues, respectively. Their authenticity was confirmed by successful expression of the coding regions inEscherichia coli. 1Dx5.3( is the shortest of the known Dx-type alleles. lDyl2.1~(**t)is also a special subunit since it has an additional cysteine in the front of the central repetitive domain. This cysteine that is not existed in previously reported Dy-type genes may be useful for improving bread wheat quality. Median-joining Network analysis indicated that lDyl2.1~(**t) may be a key site in the genealogy of the Glu-Dy.
机译：揭示了一对新的高分子量谷蛋白亚基（HMW-GS）1DX5.3〜t和lDyl2.1〜（** t），并从Ae中进行了鉴定。 tauschii登录号PI554324。 1Dx5.3〜t的SDS-PAGE带介于1Bx6和1Bx7之间，而lDyl2.1〜（** t）的迁移速率比lDyl2的迁移速率稍快。 Wx5.3'和1Dyl2.1〜（** t）的长度分别为2115 bp和1986 bp，分别编码703和660个氨基酸残基。通过在大肠杆菌中成功表达编码区，证实了它们的真实性。 1Dx5.3（是已知的Dx型等位基因中最短的一个。lDyl2.1〜（** t）也是一个特殊的亚基，因为它在中央重复结构域的前面有一个额外的半胱氨酸。这种半胱氨酸不存在以前报道的Dy型基因可能有助于提高面包小麦的品质，中位连接网络分析表明lDyl2.1〜（** t）可能是Glu-Dy家谱中的关键位点。

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