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首页> 外文期刊>Journal of Bioinformatics and Computational Biology >IDENTIFICATION OF CONSERVED FEATURES OF LAGLIDADG HOMING ENDONUCLEASES
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IDENTIFICATION OF CONSERVED FEATURES OF LAGLIDADG HOMING ENDONUCLEASES

机译：识别LAGLIDADG归巢酶的保守特征

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摘要

LAGLIDADG family of homing endonucleases are rare-cutting enzymes which recognize long target sequences and are of great interest in genome engineering. Despite advances in homing endonuclease engineering, effective methods of broadening the range of cleaved sequences are still lacking. Here, we present a study of conserved structural features of LAGLIDADG homing endonucleases that might aid further development of such methods. The protein–DNA interface of LAGLIDADG homing endonucleases differs considerably with the particular nuclease, and the analysis of conserved protein–DNA interactions could not identify any residues crucial for DNA binding and common to most nucleases of the family. For the homing endonuclease PI-SceI, a comparison of structural and experimental data derived from literature helped to identify 23 residues that are likely to be important for DNA binding. Analysis of the LAGLIDADG domain dimerization interface allowed the choosing of six positions that contributeto dimerization specificity most, while comparison of 446 sequences of LAGLIDADG endonucleases revealed groups of residues in these positions that appear to be most favorable for dimerization.
机译：LAGLIDADG归巢核酸内切酶家族是稀有切割酶，可识别长靶序列，并且在基因组工程中引起极大兴趣。尽管在归巢核酸内切酶工程化方面取得了进展，但是仍然缺乏扩大切割序列范围的有效方法。在这里，我们目前对LAGLIDADG归巢核酸内切酶保守结构特征的研究，可能有助于此类方法的进一步发展。 LAGLIDADG归巢核酸内切酶的蛋白质-DNA界面与特定核酸酶有很大差异，并且保守的蛋白质-DNA相互作用的分析无法识别任何对于DNA结合至关重要的残基，并且是该家族大多数核酸酶共有的残基。对于归巢核酸内切酶PI-SceI，比较来自文献的结构和实验数据有助于鉴定可能对DNA结合重要的23个残基。对LAGLIDADG域二聚化界面的分析允许选择最有助于二聚化特异性的六个位置，而对LAGLIDADG内切核酸酶的446个序列的比较显示，这些位置的残基组似乎最适合二聚化。

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