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首页> 外文期刊>Journal of dairy research >Growth-promoting effects of pepsin- and trypsin-treated caseinomacropeptide from bovine milk on probiotics
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Growth-promoting effects of pepsin- and trypsin-treated caseinomacropeptide from bovine milk on probiotics

机译：胃蛋白酶和胰蛋白酶处理的牛酪蛋白巨肽对益生菌的促生长作用

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摘要

Probiotic Lactobacillus and Bifidobacterium species are generally fastidious bacteria and require rich media for propagation. In milk-based media, they grow poorly, and nitrogen supplementation is required to produce high bacterial biomass levels. It has been reported that caseinomacropeptide (CMP), a 7-kDa peptide released from K-casein during renneting or gastric digestion, exhibits some growth-promoting activity for lactobacilli and bifidobacteria. During the digestive process, peptides derived from CMP are detected in the intestinal lumen. The aim of this study was to evaluate the effects of peptic and tryptic digests of CMP on probiotic lactic acid bacteria growth in de Man, Rogosa and Sharpe broth (MRS) and in milk during fermentation at 37 ℃ under anaerobic conditions. The study showed that pepsin-treated CMP used as supplements at 0·5 g/l can promote the growth of probiotics even in peptone-rich environments such as MRS. The effect was strain-dependent and evident for the strains that grow poorly in MRS, with an improvement of ＞1·5 times (P＜0·05) by addition of pepsin-treated CMP. Trypsin-treated CMP was much less efficient as growth promoter. Moreover, pepsin-treated CMP was effective in promoting the growth in milk of all probiotic lactic acid bacteria tested, with biomass levels being improved significantly, by 1·7 to 2·6 times (P＜0·05), depending on the strain. Thus, supplementation of MRS and of milk with pepsin-treated CMP would be advantageous for the production of high biomass levels for Bifidobacteria and Lactobacilli.
机译：益生菌乳杆菌和双歧杆菌属细菌一般都是挑食性细菌，需要丰富的培养基才能繁殖。在基于牛奶的培养基中，它们生长不良，并且需要补充氮才能产生高水平的细菌生物量。据报道，酪蛋白巨肽（CMP）是在凝乳酶或胃消化过程中从K-酪蛋白释放的7-kDa肽，对乳杆菌和双歧杆菌表现出一些促进生长的活性。在消化过程中，在肠腔中检测到了CMP衍生的肽。这项研究的目的是评估CMP的消化液和胰蛋白酶消化液对德曼，罗戈萨和夏普肉汤（MRS）以及牛奶在37℃厌氧条件下发酵过程中益生乳酸菌生长的影响。研究表明，即使在蛋白丰富的环境（例如MRS）中，以胃蛋白酶处理的CMP以0·5 g / l的比例用作补充剂也可以促进益生菌的生长。该效果取决于菌株，并且对于在MRS中生长较差的菌株很明显，通过添加胃蛋白酶处理的CMP可以提高＞ 1·5倍（P ＜0·05）。胰蛋白酶处理的CMP作为生长促进剂的效率低得多。此外，胃蛋白酶处理的CMP可以有效促进所有测试的益生菌乳酸菌在牛奶中的生长，其生物量水平显着提高了1·7至2·6倍（P ＜0·05），具体取决于菌株。因此，用胃蛋白酶处理的CMP补充MRS和牛奶将有利于双歧杆菌和乳杆菌产生高生物量水平。

著录项

  • 来源
    《Journal of dairy research》 |2014年第3期|319-324|共6页
  • 作者

    Gilles Robitaille; Claude P Champagne;

  • 作者单位

    Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Quebec, J2S 8E3, Canada;

    Food Research and Development Centre, Agriculture and Agri-Food Canada, St-Hyacinthe, Quebec, J2S 8E3, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Caseinomacropeptide; pepsin; trypsin; growth-promoting factor; probiotic;

    机译：酪蛋白巨肽;胃蛋白酶胰蛋白酶生长促进因子;益生菌;

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