...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Oleo Science >Metabolism and Synthesis of Lipids in the Polyunsaturated Fatty Acid-Producing Fungus Mortierella alliacea
【24h】

Metabolism and Synthesis of Lipids in the Polyunsaturated Fatty Acid-Producing Fungus Mortierella alliacea

机译：多不饱和脂肪酸真菌小孢子菌中脂质的代谢与合成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The fungal strain Mortierella alliacea YN-15 is a promising industrial producer of polyunsaturated fatty acids (PUFAs), in particular arachidonic acid. In order to more efficiently produce PUFAs, the metabolism of an externally supplied plant oil, α-linolenic acid (ALA)-rich linseed triacylglycerol (TAG), was examined, and time-dependent changes in the composition of its lipid and fatty acid metabolites were traced. Addition of linseed TAG to growing cultures resulted in a transient increase in extracellular 1,2-diacylglycerol (DAG), and even more so of 1,3-DAG, in the mycelia. This was followed by a decrease in both DAGs and an increase in TAG. Eicosapentaenoic acid (EPA), a desaturated and elongated product of ALA, accumulated to a greater extent in cellular phospholipids than in neutral lipids. Moreover, the addition of ALA in free fatty acid form to the culture led to the generation of EPA. However, EPA production was not observed upon addition of ALA-rich 1,2- or 1,3-DAG, indicating that fatty acids released from exogenous lipids were used for resynthesis of mycelial TAG. These results suggested that TAG might be hydrolyzed by extracellular lipases, whereas its synthesis might be catalyzed by intracellular enzymes. Appropriate regulation of such enzymes might be an effective strategy to enhance PUFA production under plant oil supplementation.
机译：真菌菌株小葱莫尔氏菌YN-15是多不饱和脂肪酸（PUFA），特别是花生四烯酸的有前途的工业生产商。为了更有效地生产PUFA，研究了外部供应的植物油，富含α-亚麻酸（ALA）的亚麻籽三酰甘油（TAG）的代谢，并且其脂质和脂肪酸代谢物的成分随时间变化被追踪。在生长的培养物中添加亚麻籽TAG，可导致菌丝体中的细胞外1,2-二酰甘油（DAG）瞬时增加，甚至1,3-DAG的瞬时增加。其次是两个DAG均减少，而TAG则增加。二十碳五烯酸（EPA）是ALA的一种去饱和和延长产物，在细胞磷脂中的积累程度要高于中性脂质。此外，将游离脂肪酸形式的ALA加入培养物中导致了EPA的产生。但是，添加富含ALA的1,2-或1,3-DAG时未观察到EPA的产生，这表明从外源脂质释放的脂肪酸被用于菌丝体TAG的再合成。这些结果表明TAG可能被细胞外脂肪酶水解，而其合成可能被细胞内酶催化。在补充植物油的情况下，适当调节此类酶可能是提高PUFA产量的有效策略。

著录项

  • 来源
    《Journal of Oleo Science》 |2011年第1期|p.11-17|共7页
  • 作者

    Worapol Jermsuntiea; Tsunehiro Aki; Seiji Kawamoto; Kazuhisa Ono;

  • 作者单位

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University (1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN);

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University (1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN);

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University (1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN);

    Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University (1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, JAPAN);

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    eicosapentaenoic acid; mortierella; plant oils; polyunsaturated fatty acids; single cell oil;

    机译：二十碳五烯酸;mortierella植物油;多不饱和脂肪酸;单细胞油;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号