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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Antimicrobial effects of photodynamic therapy on Staphylococcus aureus biofilm grown on a specific acrylic resin surface for ocular prostheses
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Antimicrobial effects of photodynamic therapy on Staphylococcus aureus biofilm grown on a specific acrylic resin surface for ocular prostheses

机译：光动力疗法对眼镜术丙烯酸树脂表面上生长的金黄色葡萄球菌生物膜的抗微生物作用

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Background: Photodynamic therapy (PDT) is a treatment for the specific control of oral biofilms. However, its effects on maxillofacial prostheses have been barely explored. In this study, we evaluated the antimicrobial effect of PDT using methylene blue (MB) and laser against a Staphylococcus aureus biofilm developed on the surface of scleral acrylic resin.Methods: Sixty-six specimens of acrylic resin designed for ocular prostheses were fabricated in a disk-shaped format (3 x 10 mm). S. aureus biofilm was grown on the surface of the specimens for 24 h and the disks were then treated with MB at different concentrations (25, 50, 75 or 100 mu g/mL), with or without PDT (GaAlAs diode laser; 660 nm; 100 mW; 9 J; 321.4 J.cm(-2); 3.5 W.cm(-2) and 90 s). Control groups were treated with 2% chlorhexidine gluconate (CHX) or phosphate buffered saline. After the treatments, colony forming units (CFU) were counted and the samples were qualitatively evaluated by scanning electron microscopy (SEM). Data were analyzed descriptively and by nested ANOVA and the Tukey test (alpha = .05).Results: PDT groups with MB concentrations at 75 and 100 mu g/mL formed fewer CFU compared to the other groups (P 0.001) and the 2% CHX group did not form any CFU. SEM images revealed that the surface of the polymers in these groups did not show bacterial colonies.Conclusions: PDT significantly reduced S. aureus biofilm in the scleral acrylic resin when associated with an MB dilution of 75 mu g/mL or higher. Thus, PDT can be a promising candidate for disinfecting ocular prostheses.
机译：背景：光动力疗法（PDT）是对口腔生物膜的特异性控制的治疗方法。然而，它对颌面外假肢的影响已经几乎没有探索。在这项研究中，我们评估了PDT使用亚甲基蓝（Mb）和激光对巩膜丙烯酸树脂表面开发的金黄色葡萄球菌生物膜的抗微生物效应。方法：为眼假体设计为66六个丙烯酸树脂标记磁盘形格式（3 x 10 mm）。 S.UUREUS BIOFILM在标本的表面上生长24小时，然后用不同浓度（25,50,75或100μg/ mL）的MB处理磁盘，有或没有PDT（Gaalas二极管激光器; 660 NM; 100 MW; 9 J; 321.4 J.CM（-2）; 3.5 W.CM（-2）和90秒）。用2％氯己定葡萄糖酸盐（CHX）或磷酸盐缓冲盐水处理对照组。在处理后，计算菌落形成单元（CFU），并通过扫描电子显微镜（SEM）定性评估样品。描述地分析数据，并通过嵌套的Anova和Tukey测试（Alpha = .05）。结果：与其他组相比，75和100μg/ ml的PDT组在75和100μg/ mL形成较少的CFU（P <0.001）和2 ％CHX组没有形成任何CFU。 SEM图像显示，这些组中的聚合物表面未显示细菌菌落。结论：当与75μg/ ml或更高的MB稀释相关时，PDT在巩膜丙烯酸树脂中显着降低了S.UUREUS Biofilm。因此，PDT可以是用于消毒眼假体的有希望的候选者。

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