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首页> 外文期刊>Plant and Soil >Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere
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Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere

机译：甘蔗根际中固氮杆菌和拟螺旋体菌株的分离与鉴定

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摘要

Bacteria with the ability to grow on nitrogen-free media and with nitrogenase activity under aerobic or microaerobic conditions were isolated from sugarcane roots collected from four different agricultural locations in Granada (Spain). Isolates were Gram negative rods and were identified as Azotobacter chroococcum and Azospirillum brasilense. Our results suggest that Azotobacter isolates do not have a particular affinity for sugarcane rhizospheres and that, on the contrary, Azospirillum isolates show specific association and perhaps endophytic colonization of sugarcane. However, obligate endophytes (Gluconacetobacter diazotrophicus) were not found in the apoplastic fluid of the stems and macerates extracts of sugarcane tissues with the procedure applied. Population of this microorganism might be in low number in the Spanish sugarcane varieties studied which is also discussed.
机译：从在格拉纳达（西班牙）的四个不同农业地点收集的甘蔗根中分离出具有无氮培养基生长能力并在需氧或微需氧条件下具有固氮酶活性的细菌。分离物为革兰氏阴性杆菌，被鉴定为嗜绿固氮菌和巴西固氮螺菌。我们的结果表明，固氮菌分离株对甘蔗的根际没有特殊的亲和力，相反，偶氮螺旋菌分离株显示出特定的联系，并且可能是甘蔗的内生菌落。然而，在采用该方法的甘蔗组织的茎和浸软提取物中，未发现专性内生菌（重氮糖杆菌）。在所研究的西班牙甘蔗品种中，这种微生物的数量可能很少。

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