• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of American Thoracic Society >Confocal Fluorescence Endomicroscopy of the Human Airways
【24h】

Confocal Fluorescence Endomicroscopy of the Human Airways

机译：人气共焦荧光内镜检查

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Confocal endomicroscopes aim at providing to the clinician microscopic imaging of a living tissue. The currently available micro-endoscopic devices use the principle of confocal fluorescent microscopy, in which the objective is replaced by an optical fiber and a miniaturized scanhead at the distal end of the endoscope or by a retractable bundle of optical fibers. Such systems have recently been applied to the explorations of several organs, including the gastrointestinal tract, and more recently to the proximal and distal airways in vivo. Respiratory fluorescence microendoscopes use 488 nm or 660 nm excitation laser light and thin flexible miniprobes that are introduced into the working channel of the bronchoscope. The devices have a lateral resolution of 3 μm, a field of view of 600 μm, and produce real-time imaging at 9 frames per second. For in vivo imaging, the miniprobe is applied onto the bronchial wall surface or advanced into a distal bronchiole down to the acinus. In nonsmokers, the 488-nm excitation device images the autofluorescence of the elastin that is contained in the basement membrane of the proximal airways and that participates to the axial backbone of the peripheral interstitial respiratory system. In smokers, a specific tobacco tar-induced fluorescence allows in vivo mac-rophage and alveolar wall imaging. Using 660 nm excitation and topical methylene blue, the technique enables cellular imaging of both bronchial epithelial layer and peripheral lung nodules. This article reviews the capabilities and possible limitations of confocal microendoscopy for in vivo proximal and distal lung explorations.
机译：共焦内窥镜旨在向临床医生提供活体组织的显微成像。当前可用的微内窥镜设备使用共聚焦荧光显微镜的原理，其中物镜由内窥镜远端的光纤和小型扫描头或可伸缩的光纤束代替。这样的系统近来已经被应用于包括胃肠道在内的几个器官的探查，并且最近被应用于体内的近端和远端气道。呼吸荧光微内窥镜使用488 nm或660 nm激发激光和细小的柔性微型探头，这些探头被引入支气管镜的工作通道。这些设备的横向分辨率为3μm，视野为600μm，并以每秒9帧的速度产生实时成像。对于体内成像，将微型探头应用到支气管壁表面或推进到远端的细支气管直至腺泡。在非吸烟者中，488 nm激发装置可对包含在近端气道基底膜中并参与周围组织呼吸系统轴向主干的弹性蛋白的自发荧光进行成像。在吸烟者中，特定的烟草焦油诱导的荧光允许体内巨噬细胞和肺泡壁成像。使用660 nm激发光和局部亚甲基蓝，该技术可以对支气管上皮层和周围肺结节进行细胞成像。本文回顾了共聚焦显微内窥镜在体内近端和远端肺部探查中的功能和可能的局限性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号