...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians
【24h】

Ingestion of bacterially expressed double-stranded RNA inhibits gene expression in planarians

机译：摄入细菌表达的双链RNA可抑制涡虫中的基因表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Freshwater planarian flatworms are capable of regenerating complete organisms from tiny fragments of their bodies; the basis for this regenerative prowess is an experimentally accessible stem cell population that is present in the adult planarian. The study of these organisms, classic experimental models for investigating metazoan regeneration, has been revitalized by the application of modern molecular biological approaches. The identification of thousands of unique planarian ESTs, coupled with large-scale whole-mount in situ hybridization screens, and the ability to inhibit planarian gene expression through double-stranded RNA-mediated genetic interference, provide a wealth of tools for studying the molecular mechanisms that regulate tissue regeneration and stem cell biology in these organisms. Here we show that, as in Caenorhabditis elegans, ingestion of bacterially expressed double-stranded RNA can inhibit gene expression in planarians. This inhibition persists throughout the process of regeneration, allowing phenotypes with disrupted regenerative patterning to be identified. These results pave the way for large-scale screens for genes involved in regenerative processes.
机译：淡水planar虫扁虫能够从其身体的细小片段中再生出完整的生物。这种再生能力的基础是存在于成年涡虫中的实验可接近的干细胞群体。这些微生物的研究，是研究后生动物再生的经典实验模型，已通过现代分子生物学方法的应用得以复兴。数以千计的独特涡虫EST的鉴定，加上大规模的原位杂交筛选，以及通过双链RNA介导的遗传干扰抑制涡虫基因表达的能力，为研究分子机制提供了丰富的工具调节这些生物体的组织再生和干细胞生物学。在这里，我们表明，与秀丽隐杆线虫一样，摄入细菌表达的双链RNA可以抑制涡虫中的基因表达。这种抑制作用在整个再生过程中一直存在，从而可以鉴定出再生模式被破坏的表型。这些结果为大规模筛选涉及再生过程的基因铺平了道路。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号