• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Science of the Total Environment >Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies
【24h】

Integration of five groups of POPs into one multi-analyte method for human blood serum analysis: An innovative approach within biomonitoring studies

机译：将五组POP的整合成为一种人血清血清分析的一种多分析物方法：生物监测研究中的一种创新方法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Within this study, a new analytical strategy was developed and validated for the simultaneous determination of 78 organohalogenated contaminants in human blood serum, namely 40 flame retardants (FRs) including 7 "novel" brominated and chlorinated FRs (novel FRs), 19 perfluoroalkylated substances (PFASs), 11 organochlorine pesticides (OCPs) and 8 polychlorinated biphenyls (PCBs). The integral sample preparation procedure was implemented for the isolation of non-polar compounds, based on three-step solvent extraction using a mixture of n-hexane:diethylether (9:1, v/v), followed by purification using a solid-phase extraction (SPE) on a Florisil (R) column. For isolation of more polar and lipophobic analytes, the remaining fraction from the first extraction step was further processed, using a modified QuEChERS method. Depending on the polarity and volatility of target compounds, either gas chromatography coupled to (tandem) mass spectrometry (GC-MS/(MS)), or ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry (UHPLC-MS/MS), was employed for their identification/quantification. Within the subsequent pilot study, the new validated procedure was successfully applied to the monitoring of organohalogenated contaminants in 38 samples of human blood serum obtained from Prague, Czech Republic. From 78 targeted analytes, 10 PFASs, 10 OCPs, 8 PCBs and 6 BFRs were detected in serum at concentrations above method quantification limits (MQLs). In the serum samples, the amounts of determined PFASs were in the range 0.01-8.97 ng ML-1 (mean 0.631 ng mL(-1)), OCPs and PCBs ranged from 0.1-1626 ng g(-1) lw (mean 40.0 ng g(-1) lw) and 0.1-481 ng g -1 lw (mean 63.3 ng g(-1) lw), respectively. (C) 2019 Elsevier B.V. All rights reserved.
机译：在本研究中，开发了一种新的分析策略，并验证了同时测定人血清中的78个有机卤素污染物，即40阻燃剂（FRS），包括7“新建”溴化和氯化FRS（新型FRS），19个全氟烷基化物质（ PFASS），11种有机氯杀虫剂（OCP）和8个多氯联苯（PCB）。基于使用正己烷：二乙醚（9：1，v / v）的三步溶剂萃取，实施了整体样品制备方法，用于分离非极性化合物：二乙醚（9：1，v / v），然后使用固相纯化弗洛里米尔（R）柱上的提取（SPE）。为了分离更加极性和脂肪性分析物，使用改性的QueChers方法进一步加工来自第一提取步骤的剩余部分。取决于目标化合物的极性和挥发性，偶联至（串联）质谱（GC-MS /（MS））或超高效液相色谱或偶联至三重四极杆串联质谱法（UHPLC-MS / MS的超高效液相色谱法（UHPLC-MS / MS） ），用于其识别/定量。在随后的试点研究中，新的验证程序已成功应用于从捷克共和国的布拉格获得的38种人血清样品中的有机卤化污染物的监测。从78个靶向分析物中，在血清上以上述方法量化限制（MQLS）的浓度，在血清中检测到10个PFASS，10个OCP，8个PFRS。在血清样品中，确定的PFASS的量为<0.01-8.97 ng ml-1（平均0.631 ng ml（-1）），OCP和PCB范围为<0.1-1626 ng（-1）LW（平均40.0 ng（-1）LW）和<0.1-481ng G -1 LW（平均63.3ng（-1）LW）。 （c）2019 Elsevier B.v.保留所有权利。

著录项

  • 来源
    《The Science of the Total Environment》 |2019年第1期|701-709|共9页
  • 作者

    Svarcova Andrea; Lankova Darina; Gramblicka Tomas; Stupak Michal; Hajslova Jana; Pulkrabova Jana;

  • 作者单位

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Human blood serum; Organohalogenated contaminants; Sample preparation; GC-MS; LC-MS;

    机译：人体血清;有机卤化污染物;样品制备;GC-MS;LC-MS;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号