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In Silico Pcr-rflp Of Bacillus Species: Problem-based Case Of Teaching Bioinformatics

机译：芽孢杆菌属物种的计算机Pcr-rflp中：基于问题的生物信息学教学案例

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摘要

Bioinformatics is a multi-disciplinary subject that encompasses a wide range of fields including; genomics, biotechnology, information technology, algorithms and statistics. As a result, it is important to establish a skeletal set of courses capable of providing both a general and a detailed background on all aspects of bioinformatics. In this study, a problem-based project entitled "in silico PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) of Bacillus species" was assigned to students. The project aimed to cover the background of genetic information retrieval, multiple sequence alignment, phylogenetic analysis and in silico PCR-RFLP. Initially, the 16S rRNA genes of 34 Bacillus species were retrieved from the NCBI database. The sequence data obtained were then analyzed in terms of their similarities and variation using the ClustalW software. Subsequently, the phylogenetic tree was constructed to investigate their evolutionary relationship. The results indicated that these Bacillus species could be grouped into different clades. In addition, in silico PCR-RFLP was performed to introduce students to the principles of molecular taxonomy.
机译：生物信息学是一个多学科的学科，涵盖了广泛的领域，包括：基因组学，生物技术，信息技术，算法和统计。因此，重要的是建立一套基本的课程，以提供生物信息学各个方面的一般背景和详细背景。在这项研究中，以问题为基础的项目名为“芽孢杆菌种的计算机PCR-RFLP（聚合酶链反应-限制性片段长度多态性）”已分配给学生。该项目旨在涵盖遗传信息检索，多序列比对，系统发育分析和计算机PCR-RFLP的背景。最初，从NCBI数据库中检索了34种芽孢杆菌的16S rRNA基因。然后使用ClustalW软件分析获得的序列数据的相似性和变异性。随后，构建系统发育树以研究它们的进化关系。结果表明，这些芽孢杆菌属可以分为不同的进化枝。另外，进行了计算机PCR-RFLP，以向学生介绍分子分类学的原理。

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