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Dynamics of membrane clathrin-coated structures during cytokinesis

机译：胞质分裂过程中膜网格蛋白涂层结构的动力学

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摘要

Remodeling of cell membranes takes place during motile processes such as cell migration and cell division. Defects of proteins involved in membrane dynamics, including clathrin and dynamin, disrupt cytokinesis. To understand the function of clathrin-containing structures (CCS) in cytokinesis, we have expressed a green fluorescent protein (GFP) fusion protein of clathrin light chain a (GFP-clathrin) in NRK epithelial cells and recorded images of dividing cells near the ventral surface with a spinning disk confocal microscope. Punctate GFP-CCS underwent dynamic appearance and disappearance throughout the ventral surface. Following anaphase onset, GFP-CCS between separated chromosomes migrated toward the equator and subsequently disappeared in the equatorial region. Movements outside separating chromosomes were mostly random, similar to what was observed in interphase cells. Directional movements toward the furrow were dependent on both actin filaments and microtubules, while the appearance/disappearance of CCS was dependent on actin filaments but not on microtubules. These results suggest that CCS are involved in remodeling the plasma membrane along the equator during cytokinesis. Clathrin-containing structures may also play a role in transporting signaling or structural components into the cleavage furrow.
机译：细胞膜的重塑发生在运动过程中，例如细胞迁移和细胞分裂。参与膜动力学的蛋白质缺陷（包括网格蛋白和动力蛋白）破坏胞质分裂。为了了解含网格蛋白的结构（CCS）在胞质分裂中的功能，我们已经在NRK上皮细胞中表达了网格蛋白轻链a（GFP-clathrin）的绿色荧光蛋白（GFP）融合蛋白，并记录了腹侧附近分裂细胞的图像表面用旋转盘共聚焦显微镜。点状GFP-CCS在整个腹表面经历动态出现和消失。后期开始后，分离的染色体之间的GFP-CCS移向赤道，随后在赤道区域消失。分离染色体外的运动大部分是随机的，类似于在相间细胞中观察到的运动。朝向沟的方向运动取决于肌动蛋白丝和微管，而CCS的出现/消失取决于肌动蛋白丝而不是微管。这些结果表明，CCS参与了胞质分裂过程中沿赤道的质膜的重塑。含网格蛋白的结构也可能在将信号或结构成分转运到裂解沟中发挥作用。

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