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首页> 外文期刊>Tropical Animal Health and Production >The Use of Antigen-capture Enzyme-linked Immunosorbent Assay (ELISA) for the Diagnosis of Rinderpest and Peste des Petits Ruminants in Ethiopia
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The Use of Antigen-capture Enzyme-linked Immunosorbent Assay (ELISA) for the Diagnosis of Rinderpest and Peste des Petits Ruminants in Ethiopia

机译：抗原捕获酶联免疫吸附试验（ELISA）在埃塞俄比亚牛瘟和小反刍动物的诊断中的应用

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摘要

Rinderpest had been reported in most parts of Ethiopia when the Pan African Rinderpest Campaign (PARC) was launched. As a result of intensive disease investigation and strategic vaccination, most parts of the country are now considered provisionally free, and widespread vaccination has been replaced by clinical and serological surveillance. Details of any episodes of disease are recorded and followed up after laboratory confirmation of suspected cass using antigen-capture ELISA. This paper is based on observations on the performance of the antigen detection ELISA compared to the agar gel immunodiffusion (AGID) test, which also differentiates rinderpest from peste des petits ruminants (PPR). The stability of the specific viral antigen was monitored for 4 days, and rinderpest and PPR antigens were still detected, depending on the type of specimen. Antigen capture ELISA is more rapid, sensitive and virus specific than the AGID. Even if the cold chain of the specimen is compromised for a day or two during sample collection and submission, the specimen may still be suitable for testing by ELISA.
机译：发起泛非洲牛瘟运动（PARC）时，在埃塞俄比亚的大部分地区都报告有牛瘟。由于进行了深入的疾病调查和战略疫苗接种，该国大部分地区现在被视为暂时免费，并且广泛的疫苗接种已由临床和血清学监测取代。记录任何疾病发作的详细信息，并在实验室使用抗原捕获ELISA确认可疑病例后进行随访。本文基于与琼脂凝胶免疫扩散（AGID）试验相比抗原检测ELISA的性能观察结果，该试验也将牛瘟与小反刍动物（PPR）区别开来。监测特定病毒抗原的稳定性持续4天，根据标本类型，仍可检测到牛瘟和PPR抗原。抗原捕获ELISA比AGID更快速，灵敏且具有病毒特异性。即使样品的冷链在样品收集和提交过程中受损了一天或两天，样品仍可能适合通过ELISA进行测试。

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