首页>外文期刊>生物科技>生物化学>Protein Expression and Purification >2008年第1期

Protein Expression and Purification

SCIMEDLINECA

中文名称：蛋白质表达与纯化

ISSN：1046-5928
出版周期：Monthly (except Janu

发文量：4204

期刊论文

热门论文

年度选择

更多>>

全选（0）

共105条结果

1.Heterologous high-level E. coli expression, purification and biophysical characterization of the spine-associated RapGAP (SPAR) PDZ domain

机译：脊柱相关RapGAP（SPAR）PDZ域的异源高水平大肠杆菌表达，纯化和生物物理特征
- 作者：Brown BL;Hadley M;Page R
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Solubility expression tag;
- Escherichia coli;
- PDZ domain;
- Spine-associated RapGAP (SPAR);
- MENTAL-RETARDATION;
- ESCHERICHIA-COLI;
- DENDRITIC SPINES;
- PROTEINS;
- MORPHOLOGY;
- DISORDERS;
- ANOMALIES;
- PROTEASE;
2.Expression, purification, and characterization of a functionally active Mycobacterium tuberculosis UDP-glucose pyrophosphorylase

机译：具有功能活性的结核分枝杆菌UDP-葡萄糖焦磷酸化酶的表达，纯化和鉴定
- 作者：Lai XH;Wu J;Chen S;Zhang XL;Wang HH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- UDP-glucose pyrophosphorylase;
- galU;
- Rv0993;
- Mycobacterium tuberculosis;
- virulence factor;
- ESCHERICHIA-COLI;
- IMMUNE-RESPONSES;
- PROTEIN;
- GENE;
- GALU;
- URIDYLYLTRANSFERASE;
- MEMBRANE;
- SEQUENCE;
- MUTATION;
- MUTANTS;
3.Hydrophobin (HFBI): A potential fusion partner for one-step purification of recombinant proteins from insect cells

机译：疏水蛋白（HFBI）：从昆虫细胞一步纯化重组蛋白的潜在融合伙伴
- 作者：Lahtinen T;Linder MB;Nakari-Setala T;Oker-Blom C
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
4.Maltose binding protein facilitates high-level expression and functional purification of the chemokines RANTES and SDF-1 alpha from Escherichia coli

机译：麦芽糖结合蛋白促进大肠杆菌中趋化因子RANTES和SDF-1α的高水平表达和功能纯化
- 作者：Cho HJ;Lee Y;Chang RS;Hahm MS;Kim MK;Kim YB;Oh YK
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- fusion protein;
- RANTES;
- SDF-1 alpha;
- chemokine;
- Escherichia coli;
- FUSION PROTEINS;
- ENHANCED IMMUNOGENICITY;
- AFFINITY;
- MECHANISMS;
- STRATEGIES;
- DISEASE;
- TARGETS;
- SURFACE;
- CXCR4;
- ALPHA;
5.A spontaneous direct repeat deletion in the pGEX fusion vector decreases the expression level of recombinant proteins in Escherichia coli

机译：pGEX融合载体中的自发直接重复缺失会降低重组蛋白在大肠杆菌中的表达水平
- 作者：Borgi I;Gargouri A
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- pGEX-4T-2;
- deletion;
- direct repeat sequences;
- glutathione S-transferase;
- beta-galactosidase;
- promoter;
- BETA-GALACTOSIDASE;
- PURIFICATION;
- GENE;
- REPLICATION;
- MECHANISM;
- HOMOLOGY;
- MICE;
- DNA;
6.Generation and expression of a minimal hybrid Ig-receptor formed between single domains from proteins L and G

机译：L和G蛋白的单个结构域之间形成的最小杂交Ig受体的产生和表达
- 作者：Harrison SL;Housden NG;Bottomley SP;Cossins AJ;Gore MG
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Ig-receptor;
- hybrid;
- isothermal titration calorimetry (ITC);
- fluorescence;
- immunodiffusion;
- affinity chromatography;
- protein L;
- protein G;
- IMMUNOGLOBULIN-BINDING DOMAIN;
- PEPTOSTREPTOCOCCUS-MAGNUS;
- GROUP-C;
- STREPTOCOCCI;
- PURIFICATION;
- REGIONS;
- AUREUS;
- CHAINS;
7.High-level expression, purification, and crystallization of recombinant rat leukotriene C-4 synthase from the yeast Pichia pastoris

机译：酵母巴斯德毕赤酵母中重组大鼠白三烯C-4合酶的高水平表达，纯化和结晶
- 作者：Wetterholm A;Molina DM;Nordlund P;Eshaghi S;Haeggstrom JZ
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- LTC4S;
- crystallization;
- detergent screen;
- membrane protein;
- Pichia pastoris;
- GLUTATHIONE-S-TRANSFERASE;
- ESCHERICHIA-COLI;
- PROTEIN;
- IDENTIFICATION;
- SUPERFAMILY;
- TRANSPORTER;
- STRATEGY;
- SEQUENCE;
- CLONING;
- ASTHMA;
8.Recombinant human SMOCs produced by in vitro refolding: Calcium-binding properties and interactions with serum proteins

机译：体外重折叠产生的重组人SMOC：钙结合特性和与血清蛋白的相互作用
- 作者：Novinec M;Kovacic L;Skrlj N;Turk V;Lenarcic B
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- SMOC;
- Refolding;
- C-reactive protein;
- Vitronectin;
- Fibulin;
- C-REACTIVE PROTEIN;
- INTEGRIN-LINKED KINASE;
- EXTRACELLULAR-MATRIX;
- ESCHERICHIA-COLI;
- SPARC;
- AFFINITY;
- FAMILY;
- VITRONECTIN;
- MODULATION;
- FIBULINS;
9.Efficient system for biotinylated recombinant Ets-1 production in Escherichia coli: A useful tool for studying interactions between Ets-1 and its partners

机译：在大肠杆菌中生物素化重组Ets-1生产的高效系统：研究Ets-1及其合作伙伴之间相互作用的有用工具
- 作者：Laitem C;Choul-Li S;Baillat D;Begue A;Aumercier M
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
10.Periplasmic expression and recovery of human interferon gamma in Escherichia coli

机译：人干扰素γ在大肠杆菌中的周质表达和恢复
- 作者：Hernandez VEB;Maldonado LMTP;Rivero EM;de la Rosa APB;Jimenez-Bremont JF;Acevedo LGO;Rodriguez ADL
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
11.Soluble expression and purification of the oxidoreductase component of toluene 4-monooxygenase

机译：甲苯4-单加氧酶的氧化还原酶成分的可溶性表达和纯化
- 作者：Bailey LJ;Elsen NL;Pierce BS;Fox BG
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
12.A novel immunogenic spore coat-associated protein in Bacillus anthracis: Characterization via proteomics approaches and a vector-based vaccine system

机译：炭疽芽孢杆菌中一种新型的免疫原性孢子外壳相关蛋白：通过蛋白质组学方法和基于载体的疫苗系统表征
- 作者：Liu YT;Lin SB;Huang CP;Huang CM
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- immunogenic;
- spore coat;
- Bacillus anthracis proteomics;
- vector;
- vaccine;
- ESCHERICHIA-COLI;
- PROTECTIVE IMMUNITY;
- CELL RESPONSES;
- SKIN;
- GERMINATION;
- EXPRESSION;
- CAMELYSIN;
- ADJUVANTS;
- ANTIGEN;
- CEREUS;
13.Orthogonal protein purification - Expanding the repertoire of GST fusion systems

机译：正交蛋白质纯化-扩展GST融合系统的所有功能
- 作者：Viljanen J;Larsson J;Broo KS
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
14.Expression, purification and characterization of murine Dkk1 protein

机译：鼠Dkk1蛋白的表达，纯化和表征
- 作者：Fleury D;Gillard C;Lebhar H;Vayssiere B;Touitou R;Rawadi G;Mollat P
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Dkk1 protein;
- highly expressing cell line;
- purification;
- characterization;
- Dickkopf;
- glycosylation;
- Wnt pathway;
- BONE-FORMATION;
- WNT;
- TARGETS;
- MYELOMA;
- CANCER;
15.Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system

机译：使用Ssp dnaB mini-intein系统生产重组人表皮生长因子
- 作者：Esipov RS;Stepanenko VN;Chupova LA;Boyarskikh UA;Filipenko ML;Miroshnikov AI
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- human epidermal growth factor (hEGF);
- fusion protein;
- intein;
- expression in Escherichia coli;
- ESCHERICHIA-COLI;
- FACTOR HEGF;
- PURIFICATION;
16.A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells

机译：使用HEK 293T细胞中的瞬时表达生产用于结构研究的抗体片段的管道
- 作者：Nettleship JE;Ren J;Rahman N;Berrowa NS;Hatherley D;Barclay AN;Owens RJ
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Antibody fragments;
- Fabs;
- X-ray crystallography;
- Transient expression;
- MAMMALIAN-CELLS;
- FAB FRAGMENT;
- MEMBRANE-PROTEINS;
- CRYSTAL-STRUCTURE;
- VARIABLE REGIONS;
- LIGHT-CHAINS;
- CRYSTALLIZATION;
- COMPLEX;
- EFFICIENT;
- PRIMERS;
17.Purification of the extracellular domain of the membrane protein GlialCAM expressed in HEK and CHO cells and comparison of the glycosylation

机译：在HEK和CHO细胞中表达的膜蛋白GlialCAM的胞外域的纯化和糖基化的比较
- 作者：Gaudry JP;Arod C;Sauvage C;Busso S;Dupraz P;Pankiewicz R;Antonsson B
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- GlialCAM;
- hepaCAM;
- adhesion;
- glycosylation;
- HEK;
- CHO;
- SELDI;
- ADHESION MOLECULE;
- SELECTINS;
18.Scalable purification and characterization of the extracellular domain of human autotaxin from prokaryotic cells

机译：从原核细胞可扩展纯化和纯化人自分泌运动素胞外域
- 作者：Haga A;Hashimoto K;Tanaka N;Nakamura KT;Deyashiki Y
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
19.Differentiation of human pre-adipocytes by recombinant adiponectin

机译：重组脂联素对人前脂肪细胞的分化
- 作者：Avides MD;Domingues L;Vicente A;Teixeira J
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- active adiponectin;
- baculovirus;
- adipocytes;
- differentiation;
- hormone;
- ACTIVATED PROTEIN-KINASE;
- ENDOCRINE ORGAN;
- ADIPOSE-TISSUE;
- OBESITY;
- ACRP30;
- OXIDATION;
- DOMAIN;
20.Improvement of PVX/CMV CP expression tool for display of short foreign antigens

机译：改进PVX / CMV CP表达工具以显示短的外来抗原
- 作者：Natilla A;Nemchinov LG
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
21.Production, characterization and crystallization of the Plasmodium falciparum aquaporin

机译：恶性疟原虫水通道蛋白的生产，表征和结晶
- 作者：Hedfalk K;Pettersson N;Oberg F;Hohmann S;Gordon E
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
22.Expression of Candida antarctica lipase B in Pichia pastoris and various Escherichia coli systems

机译：南极假丝酵母脂肪酶B在巴斯德毕赤酵母和各种大肠杆菌系统中的表达
- 作者：Larsen MW;Bornscheuer UT;Hult K
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Candida antarctica lipase B;
- Escherichia coli;
- Expression;
- Glycosylation;
- Pichia pastoris;
- FUNCTIONAL EXPRESSION;
- ASPERGILLUS-ORYZAE;
- PROTEINS;
- GLYCOSYLATION;
- BIOCATALYST;
- CYTOPLASM;
23.Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners

机译：融合伙伴在古菌中可溶性表达古细菌蛋白
- 作者：Kim S;Lee SB
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
24.Identification and expression of cellobiohydrolase (CBHI) gene from an endophytic fungus, Fusicoccum sp (BCC4124) in Pichia pastoris

机译：毕赤酵母内生真菌Fusicoccum sp（BCC4124）的纤维二糖水解酶（CBHI）基因的鉴定与表达
- 作者：Kanokratana P;Chantasingh D;Champreda V;Tanapongpipat S;Pootanakit K;Eurwilaichitr L
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- cellobiohydrolase;
- cellulose binding domain;
- endophytic fungus;
- Fusicoccum;
- Pichia pastoris;
- TRICHODERMA-REESEI;
- CELLULASE GENES;
- CLONING;
- DOMAINS;
25.LEKTI domain 15 is a functional Kazal-type proteinase inhibitor

机译：LEKTI域15是功能性的Kazal型蛋白酶抑制剂
- 作者：Vitzithum K;Lauber T;Kreutzmann P;Schulz A;Sommerhoff CP;Rosch P;Marx UC
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
26.Cloning, expression, purification, and characterization of AmphiTip30, a member of short-chain dehydrogenases/reductases family from the amphioxus Branchiostoma belcheri tsingtauense

机译：克隆，表达，纯化和鉴定AmphiTip30，它是来自双歧杆菌Belcheri belcheri tsingtauense的短链脱氢酶/还原酶家族的成员
- 作者：Wang SH;Zhao BS;Zhang SC;Ji HF
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- amphioxus Branchiostoma belcheri;
- characterization;
- cloning;
- dehydrogenases/reductases activity;
- expression;
- TIP30;
- TAT-ACTIVATED TRANSCRIPTION;
- SEQUENCE-ANALYSIS;
- VERTEBRATE LIVER;
- TIP30;
- METASTASIS;
- IDENTIFICATION;
- COACTIVATOR;
- COFACTOR;
- ORIGIN;
- GROWTH;
27.Enhanced protein expression in the baculovirus/insect cell system using engineered SUMO fusions

机译：使用工程SUMO融合蛋白增强杆状病毒/昆虫细胞系统中的蛋白质表达
- 作者：Liu L;Spurrier J;Butt TR;Strickler JE
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
28.An E. coli expression system optimized for DELLA proteins

机译：针对DELLA蛋白优化的大肠杆菌表达系统
- 作者：Sun XL;Frearson N;Kirk C;Jones WT;Harvey D;Rakonjac J;Foster T;Al-Samarrai T
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- DELLA proteins;
- E. coli expression;
- gibberellin signal transduction pathway;
- plant proteins;
- ESCHERICHIA-COLI;
- PROTEOLYTIC DEGRADATION;
- FUSION PROTEIN;
- GIBBERELLIN;
- GROWTH;
- PURIFICATION;
- REPRESSOR;
- ANTIBODY;
- ENCODES;
- BINDING;
29.GMP production and characterization of the bivalent anti-human T cell immunotoxin, A-dmDT390-bisFv(UCHTl) for phase I/II clinical trials

机译：I / II期临床试验的GMP产生和二价抗人T细胞免疫毒素A-dmDT390-bisFv（UCHTl）的表征
- 作者：Woo JH;Liu JS;Kang SH;Singh R;Park SK;Su YP;Ortiz J;Neville DM;Willingham MC;Frankel AE
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- diphtheria toxin;
- UCHT1;
- CD3 positive;
- Pichia pastoris;
- PICHIA-PASTORIS;
- CHAIN IMMUNOTOXIN;
- DIPHTHERIA-TOXIN;
- EFFICACY;
- LYMPHOMA;
- PROTEIN;
- SECRETION;
- DEPLETION;
- TOXICITY;
- MUTANTS;
30.Expression, purification and characterization of a recombinant Lipomyces starkey dextranase in Pichia pastoris

机译：重组毕赤酵母星葡聚糖酶在巴斯德毕赤酵母中的表达，纯化和鉴定
- 作者：Chen L;Zhou XS;Fan WM;Zhang YX
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- dextranase;
- Lipomyces starkeyi;
- Pichia pastoris;
- expression;
- purification;
- LEUCONOSTOC-MESENTEROIDES;
- FERMENTATION;
- MINIOLUTEUM;
- ENZYMES;
- CLONING;
- GENE;
31.Cloning, purification and initial characterization of E. coli McrA, a putative 5-methylcytosine-specific nuclease

机译：大肠杆菌McrA（一种假定的5-甲基胞嘧啶特异性核酸酶）的克隆，纯化和初步表征
- 作者：Mulligan EA;Dunn JJ
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
32.Large scale isolation and purification of soluble RAGE from lung tissue

机译：从肺组织中大规模分离和纯化可溶性RAGE
- 作者：Englert JM;Ramsgaard L;Valnickova Z;Enghild JJ;Oury TD
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- RAGE;
- sRAGE;
- purification;
- mouse;
- bovine;
- lung;
- GLYCATION END-PRODUCTS;
- RECEPTOR;
- ATHEROSCLEROSIS;
- SRAGE;
33.Ligand-independent assembly of purified soluble magic roundabout (Robo4), a tumor-specific endothelial marker

机译：纯化的可溶性魔术环岛（Robo4）的配体独立装配，一种肿瘤特异性内皮标记
- 作者：Yoshikawa M;Mukai Y;Okada Y;Yoshioka Y;Tsunoda SI;Tsutsumi Y;Okada N;Aird WC;Doi TF;Nakagawa SS
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- magic roundabout;
- Robo4;
- angiogenesis;
- vessel marker;
- multimer;
- ANGIOGENESIS;
- EXPRESSION;
- RECEPTOR;
- CANCER;
- GLYCOSYLATION;
- MIGRATION;
- SYSTEMS;
- PROTEIN;
- COMPLEX;
- FAMILY;
34.Recombinant bovine dihydrofolate reductase produced by mutagenesis and nested PCR of murine dihydrofolate reductase cDNA

机译：鼠二氢叶酸还原酶cDNA的诱变和巢式PCR产生的重组牛二氢叶酸还原酶
- 作者：Cody V;Mao Q;Queener SF
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Bovine;
- Murine;
- Dihydrofolate reductase;
- PCR;
- Kinetics;
- Crystallization;
- CHICKEN LIVER;
- ESCHERICHIA-COLI;
- COENZYME BINDING;
- TEA CATECHINS;
- INHIBITION;
- 5-(SUBSTITUTED-BENZYL)-2;
- 4-DIAMINOPYRIMIDINES;
- CRYSTALLIZATION;
- TRIMETHOPRIM;
- PURIFICATION;
- KINETICS;
35.Development of a method for expression and purification of the regulatory C2-like domain of human 5-lipoxygenase

机译：表达和纯化人5-脂氧合酶的调节性C2样结构域的方法的开发
- 作者：Michel AAY;Steinhilber D;Werz O
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- 5-lipoxygenase;
- leukotriene;
- C2 domain;
- BETA-BARREL DOMAIN;
- ENZYME-ACTIVITY;
- LEUKOTRIENE SYNTHESIS;
- NUCLEAR-MEMBRANE;
- PROTEIN;
- IRON;
- LOCALIZATION;
- INHIBITION;
- CALCIUM;
- KINASES;
36.Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography

机译：单克隆抗体偶联亲和柱色谱法纯化高纯度，高产量的GFP融合蛋白
- 作者：Zhuang R;Zhang Y;Zhang R;Song C;Yang K;Yang A;Jin B
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- affinity purification;
- GFP;
- high purity;
- monoclonal antibody;
- IMMUNOAFFINITY CHROMATOGRAPHY;
- FLUORESCENT PROTEIN;
- EXPRESSION;
- CELLS;
- EGFP;
37.One-step affinity tag purification of full-length recombinant human AP-1 complexes from bacterial inclusion bodies using a polycistronic expression system

机译：使用多顺反子表达系统一步一步从细菌包涵体中纯化全长重组人AP-1复合物的亲和标签
- 作者：Wang WM;Lee AY;Chiang CM
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- AP-1;
- FLAG tag;
- hexahistidine tag;
- inclusion bodies;
- affinity purification;
- DNA-BINDING DOMAINS;
- TRANSCRIPTIONAL REGULATION;
- PROTEIN COMPLEXES;
- POLYMERASE-II;
- IN-VITRO;
- JUN;
- FOS;
- ACTIVATION;
- TRANSFORMATION;
- HETERODIMERS;
38.Construction of Escherichia coli dnaK-deletion mutant infected by lambda DE3 for overexpression and purification of recombinant GrpE proteins

机译：λDE3感染的大肠杆菌dnaK缺失突变体的构建，用于重组GrpE蛋白的过表达和纯化
- 作者：Sugimoto S;Higashi C;Yoshida H;Sonomoto K
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- molecular chaperone;
- DnaK;
- GrpE;
- Escherichia coli expression system;
- MOLECULAR CHAPERONE DNAK;
- TEMPERATURE;
- EXPRESSION;
- HSP70;
- GENE;
- ATP;
39.A new protocol for high-yield purification of recombinant human CXCL8((3-72))K11R/G31P expressed in Escherichia coli

机译：高产纯化在大肠杆菌中表达的重组人CXCL8（（3-72））K11R / G31P的新协议
- 作者：Cheng HT;Huang KC;Yu HY;Gao KJ;Zhao XX;Li F;Town J;Gordon JR;Cheng JW
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- CXCL8;
- antagonist;
- expression;
- purification;
- lipopolysaccharide;
- endotoxin;
- PROTEIN PREPARATIONS;
- ENDOTOXIN TOLERANCE;
- CHEMOKINE RECEPTORS;
- NEUTROPHIL CXCR1;
- INTERLEUKIN-8;
- REMOVAL;
- BINDING;
- RESIDUES;
- SPECIFICITY;
- ANALOGS;
40.The molecular design of a recombinant antimicrobial peptide CP and its in vitro activity

机译：重组抗菌肽CP的分子设计及其体外活性
- 作者：Niu MF;Li X;Wei JC;Cao RB;Zhou B;Chen PY
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- a synthetic antimicrobial peptide cp gene;
- Pichia pastoris x-33;
- expression;
- stability;
- antibacterial activity;
- YEAST PICHIA-PASTORIS;
- PROTEIN EXPRESSION;
- ANTIBODY;
- OUTER;
41.Cloning, expression, purification and characterization of recombinant glutathione-S-transferase from Xylella fastidiosa

机译：耐生小球藻重组谷胱甘肽-S-转移酶的克隆，表达，纯化及鉴定
- 作者：Travensolo RF;Garcia W;Muniz JRC;Caruso CS;Lemos EGM;Carrilho E;Araujo APU
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
42.Matrix-assisted refolding and redox properties of WhiB3/Rv3416 of Mycobacterium tuberculosis H37Rv

机译：结核分枝杆菌H37Rv WhiB3 / Rv3416的基质辅助重折叠和氧化还原特性
- 作者：Alam MS;Agrawal P
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
43.High yield purification of soluble guanylate cyclase from bovine lung

机译：从牛肺中高纯度纯化可溶性鸟苷酸环化酶
- 作者：Mathis KJ;Emmons TL;Curran DF;Day JE;Tomasselli AG
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
44.Recombinant expression of twelve evolutionarily diverse subfamily lot aminotransferases

机译：十二种进化上不同的亚科很多氨基转移酶的重组表达
- 作者：Muratore KE;Srouji JR;Chow MA;Kirsch JF
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
45.A new Gateway (R) vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis

机译：一种新的Gateway（R）载体和表达方案，可在耻垢分枝杆菌中快速高效地表达重组蛋白
- 作者：Goldstone RM;Moreland NJ;Bashiri G;Baker EN;Lott JS
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- recombinant protein expression;
- inclusion bodies;
- Mycobacterium tuberculosis;
- Mycobacterium smegmatis;
- Gateway;
- autoinduction media;
- PURIFICATION;
- CRYSTALLIZATION;
- CLONING;
46.Yersinia pestis YopD 150-287 fragment is partially unfolded in the native state

机译：鼠疫耶尔森氏菌YopD 150-287片段在天然状态下部分展开
- 作者：Raab R;Swietnicki W
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Yersinia pestis;
- YopD;
- SycD;
- translocator;
- molten globule;
- PROTEIN SECONDARY STRUCTURE;
- III SECRETION SYSTEM;
- AMINO-ACID-SEQUENCE;
- STRUCTURE PREDICTION;
- MOLTEN GLOBULE;
- AMPHIPATHIC DOMAIN;
- V-ANTIGEN;
- CHAPERONE;
- BINDING;
- TRANSLOCON;
47.Bacterial production of latarcin 2a, a potent antimicrobial peptide from spider venom

机译：细菌毒Latarcin 2a的细菌生产
- 作者：Shlyapnikov YM;Andreev YA;Kozlov SA;Vassilevski AA;Grishin EV
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
48.Cloning, expression and characterization of a thermotolerant endoglucanase from Syncephalastrum racemosum (BCC18080) in Pichia pastoris

机译：巴斯德毕赤酵母耐热性内切葡聚糖酶（BCC18080）的克隆，表达及鉴定
- 作者：Wonganu B;Pootanakit K;Boonyapakron K;Champreda V;Tanapongpipat S;Eurwilaichitr L
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Syncephalastrum racemosum;
- endoglucanase;
- thermotolerant;
- Pichia pastoris expression;
- MOLECULAR-CLONING;
- HUMICOLA-INSOLENS;
- RHIZOPUS-ORYZAE;
- CELLULASE;
- GENE;
- PURIFICATION;
- XYLANASE-10;
- ENZYMES;
49.Producing human mechano growth factor (MGF) in Escherichia coli

机译：在大肠杆菌中产生人类机械生长因子（MGF）
- 作者：Kuznetsova TV;Schulga AA;Wulfson AN;Keruchenko JS;Ermolyuk YS;Keruchenko ID;Tikhonov RV;Lisitskaya KV;Makarov AA;Chobotova K
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- growth factor;
- muscles;
- myoblast;
- insulin-like growth factor;
- refolding;
- recombinant protein;
- proliferation test;
- I SPLICE VARIANTS;
- SKELETAL-MUSCLE;
- IGF-I;
- HUMAN INSULIN;
- EXPRESSION;
- PEPTIDE;
- STRETCH;
- CLONING;
- GENE;
50.Expression, purification, and characterization of multiple, multifunctional human glucocorticoid receptor proteins

机译：多种多功能人糖皮质激素受体蛋白的表达，纯化和表征
- 作者：Simmons CA;Bledsoe RK;Guex N;Pearce KH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Nuclear receptor;
- Glucocorticoid receptor;
- DNA binding;
- Coactivator protein binding;
- Steroid binding;
- HEAT-SHOCK-PROTEIN;
- LIGAND-BINDING DOMAIN;
- RESPONSE ELEMENT;
- IDENTIFICATION;
- DIMERIZATION;
- RECOGNITION;
- MECHANISMS;
- CHAPERONES;
- SYSTEM;
- GENE;
51.Recombinant production and characterization of the carbohydrate recognition domain from Atlantic salmon C-type lectin receptor C (SCLRC)

机译：大西洋鲑C型凝集素受体C（SCLRC）的碳水化合物识别结构域的重组生产和表征
- 作者：Soanes KH;Ewart KV;Mattatall NR
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
52.Codon optimization can improve expression of human genes in Escherichia coli: A multi-gene study

机译：密码子优化可以改善人类基因在大肠杆菌中的表达：一项多基因研究
- 作者：Burgess-Brown NA;Sharma S;Sobott F;Loenarz C;Oppermann U;Gileadi O
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
53.Optimized gene synthesis, expression and purification of active salivary plasminogen activator alpha 2 (DSPAot2) of Desmodus rotundus in Pichia pastoris

机译：巴斯德毕赤酵母中唾液纤溶酶原活性唾液纤溶酶原激活物α2（DSPAot2）的基因合成，表达和纯化的优化
- 作者：Wei ZR;Wang YJ;Li GQ;Li XK;Liu DH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- vampire bat;
- plasminogen activator alpha 2;
- gene synthesis;
- optimized codon;
- DESMOTEPLASE;
- CELLS;
- OVEREXPRESSION;
- DSPA-ALPHA-1;
- PROTEOLYSIS;
- SECRETION;
- ANIMALS;
- PATHWAY;
- CLONING;
54.pCold-GST vector: A novel cold-shock vector containing GST tag for soluble protein production

机译：pCold-GST载体：含有GST标签的新型冷休克载体，用于可溶性蛋白生产
- 作者：Hayashi K;Kojima C
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Protein expression;
- E. coli;
- Cold-shock vector;
- GST;
- Soluble protein production;
- NMR;
- ESCHERICHIA-COLI;
- PURIFICATION;
- EXPRESSION;
- EFFICIENT;
- DOMAINS;
- FUSION;
55.Expression and purification of the recombinant membrane protein YidC: A case study for increased stability and solubility

机译：重组膜蛋白YidC的表达和纯化：提高稳定性和溶解度的案例研究
- 作者：Molina DM;Lundback AK;Niegowski D;Eshaghi S
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Membrane protein insertion system;
- Stability optimization;
- Analytical size exclusion chromatography;
- Gel filtration;
- Buffer screen;
- ESCHERICHIA-COLI;
- INSERTION;
- HOMOLOG;
- TRANSPORTER;
- DETERGENT;
- BACTERIA;
- ALBINO3;
- DOMAIN;
56.Increasing the homogeneity, stability and activity of human serum albumin and interferon-alpha 2b fusion protein by linker engineering

机译：通过接头工程提高人血清白蛋白和干扰素-α2b融合蛋白的均一性，稳定性和活性
- 作者：Zhao HL;Yao XQ;Xue C;Wang Y;Xiong XH;Liu ZM
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- linker engineering;
- HSA fusion protein;
- Pichia pastoris;
- CHRONIC HEPATITIS-C;
- PEPTIDES;
57.Expression of the extracellular region of the human interleukin-4 receptor alpha chain and interleukin-13 receptor alpha 1 chain by a silkworm-baculovirus system

机译：蚕杆状病毒系统表达人白细胞介素4受体α链和白介素13受体α1链的胞外区
- 作者：Honjo E;Shoyama Y;Tamada T;Shigematsu H;Hatanaka T;Kanaji S;Arima K;Ito Y;Izuhara K;Kuroki R
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- IL-13;
- IL-13 receptor alpha 1 chain;
- IL-4 receptor alpha chain;
- silkworm;
- Fc fusion;
- HIGH-LEVEL EXPRESSION;
- BOMBYX-MORI;
- IL-4 RECEPTOR;
- CYTOPLASMIC DOMAIN;
- SIGNALING COMPLEX;
- ILE50VAL VARIANT;
- ATOPIC ASTHMA;
- IGE SYNTHESIS;
- PROTEIN;
- BINDING;
58.A new feasible method for fibrinogen purification based on the affinity of Staphylococcus aureus clumping factor A to fibrinogen

机译：一种基于金黄色葡萄球菌聚集因子A与纤维蛋白原亲和力的新型可行的纤维蛋白原纯化方法
- 作者：Liu CZ;Cheng HJ;Chang LY
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- clumping factor A;
- fibrinogen;
- affinity purification;
- CROSS-LINKING;
- GAMMA-CHAINS;
- SNAKE-VENOM;
- PROTEIN;
- CHROMATOGRAPHY;
- CLFA(221-550);
- INTEGRIN;
- REGIONS;
- SEGMENT;
- MICE;
59.Cloning, expression, purification and characterization of the stress kinase YeaG from Escherichia coli

机译：大肠杆菌应激激酶YeaG的克隆，表达，纯化及鉴定
- 作者：Tagourti J;Landoulsi A;Richarme G
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- protein kinase;
- environmental stresses;
- bacteria;
- Escherichia coli;
- SER/THR PROTEIN-KINASES;
- PHOSPHORYLATION;
- IDENTIFICATION;
- TUBERCULOSIS;
- ACTIVATION;
- BACTERIA;
- GENES;
60.A new purification strategy for fraction 1 capsular antigen and its efficacy against Yersinia pestis virulent strain challenge

机译：1级荚膜抗原的新纯化策略及其抗鼠疫耶尔森氏菌强毒株的功效
- 作者：Wang T;Qi ZZ;Wu BC;Zhu ZW;Yang YH;Cui BZ;Dai RX;Zhang QW;Qiu YF;Wang ZY
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Yersinia pestis;
- F1 antigen;
- purification;
- protection;
- F1 ANTIGEN;
- PNEUMONIC PLAGUE;
- PROTECTION;
- VACCINE;
- MODEL;
- MICE;
61.Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)-characterization of its phosphatase activity and interaction with Golli-BG21

机译：重组鼠Golli相互作用蛋白（GIP）活性变体的表达和纯化-表征其磷酸酶活性以及与Golli-BG21的相互作用
- 作者：Bamm VV;Harauz G
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
62.Expression, purification, and characterization of an immunotoxin containing a humanized anti-CD25 single-chain fragment variable antibody fused to a modified truncated Pseudomonas exotoxin A

机译：包含与修饰的截短的假单胞菌外毒素A融合的人源化抗CD25单链片段可变抗体的免疫毒素的表达，纯化和表征
- 作者：Wang HJ;Dai JX;Li BHX;Fan K;Peng L;Zhang DP;Cao ZG;Qlan WZ;Wang H;Zhao J
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- immunotoxin;
- inclusion bodies;
- refolding;
- vascular leak syndrome;
- VASCULAR LEAK SYNDROME;
- ESCHERICHIA-COLI;
- RECOMBINANT IMMUNOTOXIN;
- INCLUSION-BODIES;
- IN-VITRO;
- PROTEINS;
- INTERLEUKIN-2;
- AERUGINOSA;
- BINDING;
- TOXINS;
63.Expression, purification and crystallization of cysteine-rich human protein muskelin in Escherichia coli

机译：富含半胱氨酸的人蛋白Muskelin在大肠杆菌中的表达，纯化和结晶
- 作者：Kiedzierska A;Czepczynska H;Smietana K;Otlewski J
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- beta-barrel;
- beta-propeller;
- SUMO;
- crystallization;
- muskelin;
- CIRCULAR-DICHROISM SPECTRA;
- SECONDARY STRUCTURE;
- MOTIF;
- PHOSPHATASES;
- DOMAINS;
- ENZYME;
64.Improvement in soluble expression levels of a diabody by exchanging expression vectors

机译：通过交换表达载体改善双抗体的可溶性表达水平
- 作者：Liu F;Chen Z;Jiang WG;Yang CZ;Xiong DS;Zhu ZP
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
65.Defining the minimal interacting regions of the tight junction protein MAGI-1 and HPV16 E6 oncoprotein for solution structure studies

机译：定义紧密连接蛋白MAGI-1和HPV16 E6癌蛋白的最小相互作用区域以进行溶液结构研究
- 作者：Charbonnier S;Stier G;Orfanoudakis G;Kieffer B;Atkinson RA;Trave G
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
66.A high-throughput microtiter plate-based screening method for the detection of full-length recombinant proteins

机译：基于高通量微量滴定板的筛选方法，用于检测全长重组蛋白
- 作者：Mack M;Burger M;Pietschmann P;Hock B
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- protein production;
- Escherichia coli;
- Luminex (R) xMAP (TM) bead technology;
- HIGH-LEVEL EXPRESSION;
- ESCHERICHIA-COLI;
- HETEROLOGOUS PROTEINS;
- STRATEGIES;
- CYTOPLASM;
- CULTURES;
- SYSTEM;
67.Escherichia coli expression and purification of four antimicrobial peptides fused to a family 3 carbohydrate-binding module (CBM) from Clostridium thermocellum

机译：大肠杆菌表达和纯化与热纤梭菌第3族碳水化合物结合模块（CBM）融合的四种抗菌肽
- 作者：Guerreiro CIPD;Fontes CMGA;Gama M;Domingues L
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- cationic amphiphilic antimicrobial peptides;
- PMAP-23;
- CBM3;
- protein fusion;
- Clostridium thermocellum;
- Escherichia coli;
- HIGH-LEVEL EXPRESSION;
- ANTIBACTERIAL PEPTIDE;
- CELLULOSE;
- CELLS;
- PMAP-23;
- DESIGN;
- DOMAIN;
- SYSTEM;
- LECTIN;
68.Expression and purification of recombinant human interleukin-18 protein using a yeast expression system

机译：使用酵母表达系统表达和纯化重组人白介素18蛋白
- 作者：Yang LL;An XM;Wei F;Liu H;Li H;Yu JP;Ren XB
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Interleukin-18;
- Pichia pastoris;
- Expression;
- Purification;
- IFN-GAMMA PRODUCTION;
- PICHIA-PASTORIS;
- T-CELLS;
- IL-18;
- CYTOTOXICITY;
- CYTOKINE;
- ANTIGEN;
- CLONING;
- TH1;
69.In vivo aggregation of bovine beta-lactoglobulin is affected by Cys at position 121

机译：牛β-乳球蛋白的体内聚集受到121位半胱氨酸的影响
- 作者：Invernizzi G;Annoni E;Natalello A;Doglia SM;Lotti M
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
70.Strict regulation of gene expression from a high-copy plasmid utilizing a dual vector system

机译：利用双重载体系统严格控制高拷贝质粒的基因表达
- 作者：Gruber DF;Pieribone VA;Porton B;Kao HT
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
71.Refinement in the production and purification of recombinant HCMV IE1-pp65 protein for the generation of epitope-specific T cell immunity

机译：精细生产和纯化重组HCMV IE1-pp65蛋白以产生表位特异性T细胞免疫
- 作者：Nguyen THO;Mifsud NA;Stewart LA;Rose MJ;Etto TL;Williamson NA;Purcell AW;Kotsimbos T;Schwarer AP
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- HCMV;
- IE1-pp65;
- protein purification;
- ALLOGENEIC BONE-MARROW;
- HUMAN CYTOMEGALOVIRUS;
- DENDRITIC CELLS;
- CROSS-PRESENTATION;
- TRANSPLANTATION;
- LYMPHOCYTES;
- VIRUS;
- IDENTIFICATION;
- INFECTION;
- RESPONSES;
72.Biophysical and biochemical characterization of recombinant human Pop2 deadenylase

机译：重组人Pop2腺苷酸酶的生物物理和生化特性
- 作者：Liu WF;Yan YB
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
73.Addressing Shewanella oneidensis 'cytochromome': The first step towards high-throughput expression of cytochromes c

机译：解决Shewanella oneidensis“ cytochromome”：细胞色素c高通量表达的第一步
- 作者：Londer YY;Giuliani SE;Peppler T;Collart FR
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
74.Identification of a novel beta-N-acetylhexosaminidase (Pcb-NAHAI) from marine zoanthid Palythoa caribaeorum (Cnidaria, Anthozoa, Zoanthidea)

机译：鉴定了一种新的β-N-乙酰基己糖胺酶（Pcb-NAHAI），其来自海洋动物拟南芥Palythoa caribaeorum（Cnidaria，Anthozoa，Zoanthidea）
- 作者：Souza DSL;Grossi-De-Sa MF;Silva LP;Franco OL;Gomes-Junior JE;Oliveira GR;Rocha TL;Magalhaes CP;Marra BM;Grossi-de-Sa M
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
75.Expression and characterization of human glycosylated interleukin-1 receptor antagonist in Pichia pastoris

机译：人糖基化白介素1受体拮抗剂在毕赤酵母中的表达和鉴定
- 作者：Hamilton BS;Brede Y;Tolbert TJ
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- interleukin-1 receptor antagonist;
- glycoprotein;
- Pichia pastoris;
- interleukin-1;
- yeast;
- INTERLEUKIN-1 RECEPTOR ANTAGONIST;
- SACCHAROMYCES-CEREVISIAE;
- PROTEIN;
- PURIFICATION;
- CELLS;
76.Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability

机译：在酿酒酵母中表达的具有较高糖基化和稳定性的里氏木霉重组内切葡聚糖酶的纯化和鉴定
- 作者：Qin YQ;Wei XM;Liu XM;Wang TH;Qu YB
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Trichoderma reesei;
- endoglucanase;
- Saccharomyces cerevisiae;
- glycosylation;
- stability;
- PICHIA-PASTORIS;
- YEAST;
- SECRETION;
- CELLULOSE;
- GENE;
- ENDO-BETA-1;
- 4-GLUCANASE;
- IDENTIFICATION;
- ENHANCEMENT;
- HYDROLYSIS;
- CLONING;
77.Transient expression of HPV16 E7 peptide (aa 44-60) and HPV16 L2 peptide (aa 108-120) on chimeric potyvirus-like particles using Potato virus X-based vector

机译：HPV16 E7肽（aa 44-60）和HPV16 L2肽（aa 108-120）使用基于马铃薯X病毒的载体在嵌合马铃薯红病毒样颗粒上的瞬时表达
- 作者：Cerovska N;Hoffmeisterova H;Pecenkova T;Moravec T;Synkova H;Plchova H;Veleminsky J
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- epitopes expression;
- Human papillomavirus;
- transient expression;
- potyvirus-like particles;
- synergistic infection;
- TRANSGENIC PLANTS;
- GENE;
- RNA;
- REPLICATION;
- SUPPRESSORS;
- TRANSCRIPTS;
- ANTIBODIES;
- SEQUENCES;
- PROTEINS;
- VACCINE;
78.A fast and simple method to prepare the FKBP-rapamycin binding domain of human target of rapamycin for NMR binding assays

机译：一种快速简便的方法，用于制备雷帕霉素人靶标的FKBP-雷帕霉素结合域，用于NMR结合测定
- 作者：Dames SA
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- target of rapamycin;
- TOR;
- FKBP;
- refolding;
- inclusion body;
- HPLC;
- NMR;
- CELL-GROWTH CONTROL;
- TERNARY COMPLEX;
- CANCER-THERAPY;
- MTOR;
- PROTEIN;
- STABILITY;
- DISEASE;
79.Effect of location of the His-tag on the production of soluble and functional Buthus martensii Karsch insect toxin

机译：组氨酸标签的位置对可溶性和功能性马氏Kar虫昆虫毒素产生的影响
- 作者：Xu CG;Fan XJ;Fu YJ;Liang AH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
80.Baculovirus-mediated expression, purification, and characterization of a fully activated catalytic kinase domain construct of the 70 kDa 40S ribosomal protein S6 kinase-1 alpha II isoform (S6K1 alpha II)

机译：杆状病毒介导的70 kDa 40S核糖体蛋白S6激酶-1 alpha II亚型（S6K1 alpha II）的完全活化的催化激酶结构域构建体的表达，纯化和表征
- 作者：Keshwani MM;Ross DB;Ragan TJ;Harris TK
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- S6K1-T389E;
- phosphoinositide-dependent protein kinase-1;
- PDK1;
- Sf9 insect cells;
- baculoviral coinfection;
- ESI-TOF;
- MonoQ anion exchange;
- protein phosphorylation;
- BINDING POCKET;
- CELL-GROWTH;
- PHOSPHORYLATION;
- PDK1;
- MTOR;
- TOR;
81.Efficient production and characterization of Bacillus anthracis lethal factor and a novel inactive mutant rLFm-YF

机译：炭疽芽孢杆菌致死因子和新型无活性突变体rLFm-YF的高效生产和表征
- 作者：Cao S;Liu Z;Guo A;Li Y;Zhang C;Gaobing W;Chunfang F;Tan Y;Chen H
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Bacillus anthracis;
- lethal factor;
- anthrax;
- lethal toxin;
- protein purification;
- PROTECTIVE ANTIGEN;
- STEP PURIFICATION;
- TOXIN;
- IMMUNIZATION;
- EXPRESSION;
- CHALLENGE;
- SURVIVAL;
- BINDING;
- DOMAIN;
82.Purification and characterization of a recombinant human testican-2 expressed in baculovirus-infected Sf9 insect cells

机译：在杆状病毒感染的Sf9昆虫细胞中表达的重组人testican-2的纯化和鉴定
- 作者：Pavsic M;Vito T;Lenarcic B
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
83.Development of a bacterial cloning vector for expression of scorpion toxins for biotechnological studies

机译：用于生物技术研究的表达蝎毒素的细菌克隆载体的开发
- 作者：Stehling EG;da Silveira WD;Campos TA;Brocchi M;Pertinhez TA;Spisni A
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- molecular cloning;
- toxin;
- scorpion;
- SODIUM-CHANNELS;
- K+ CHANNELS;
- VENOM;
- PURIFICATION;
- NEUTRALIZES;
- PEPTIDES;
84.A step-wise approach significantly enhances protein yield of a rationally-designed agonist antibody fragment in E-coli

机译：逐步方法可显着提高大肠杆菌中合理设计的激动剂抗体片段的蛋白质产量
- 作者：Lin B;Renshaw MW;Autote K;Smith LM;Calveley P;Bowdish KS;Frederickson S
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- antibody;
- agonist;
- CODON USAGE BIAS;
- BACTERIAL EXPRESSION;
- FAB FRAGMENTS;
- FUNCTIONAL EXPRESSION;
- MEMBRANE-PROTEINS;
- HEAVY-CHAIN;
- OPTIMIZATION;
- PURIFICATION;
- LEVEL;
- CHAPERONE;
85.Heterologous expression of functionally active enterolysin A, class III bacteriocin from Enterococcus faecalis, in Escherichia coli

机译：粪肠球菌的功能性肠溶素A（III类细菌素）在大肠杆菌中的异源表达
- 作者：Nigutova K;Serenova L;Piknova M;Javorsky P;Pristas P
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- class III bacteriocin;
- enterolysin A;
- heterologous expression;
- Escherichia coli;
- GRAM-POSITIVE BACTERIA;
- LYSIS GENES;
- PURIFICATION;
- CLONING;
- SEQUENCE;
- FAECIUM;
- PROTEIN;
- DOMAIN;
86.Effective high-throughput overproduction of membrane proteins in Escherichia coli

机译：大肠杆菌中膜蛋白的有效高通量过量生产
- 作者：Gordon E;Horsefield R;Swarts HGP;de Pont JJHHM;Neutze R;Snijder A
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Membrane proteins;
- His-tag;
- Structural genomics;
- Escherichia coli;
- Auto-induction;
- Protein production;
- Legionella pneumophila;
- Legionnaires' disease;
- LEGIONELLA-PNEUMOPHILA;
- CRYSTAL-STRUCTURE;
- EXPRESSION;
- CHANNEL;
- PURIFICATION;
- RECEPTORS;
- CLONING;
- GROWTH;
87.Optimum conditions for production and purification of human papillomavirus type 16 L1 protein from Saccharomyces cerevisiae

机译：从酿酒酵母中生产和纯化人乳头瘤病毒16型L1蛋白的最佳条件
- 作者：Park MA;Kim HJ;Kim HJ
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
88.Optimized in vitro and in vivo expression of proteorhodopsin: A seven-transmembrane proton pump

机译：蛋白视紫红质的体外和体内优化表达：七跨膜质子泵
- 作者：Gourdon P;Alfredsson A;Pedersen A;Mahnerberg E;Nyblom M;Widell M;Berntsson R;Pinhassi J;Braiman M;Hansson O
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
89.Cloning, purification and comparative structural analysis of two hypothetical proteins from Mycobacterium tuberculosis found in the human granuloma during persistence and highly up-regulated under carbon-starvation conditions

机译：持续存在并在碳饥饿条件下高度上调的人类肉芽肿中发现的两种结核分枝杆菌假想蛋白质的克隆，纯化和比较结构分析
- 作者：Luthra A;Malik SS;Ramachandran R
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
90.Expression, purification and characterization of recombinant mitochondrial topoisomerase II of kinetoplastid Crithidia fasciculata in High-five insect cells

机译：运动型胶体Crisidia fasciculata的重组线粒体拓扑异构酶II在高五个昆虫细胞中的表达，纯化和鉴定
- 作者：Wang YZ;Tsai YC;Urena-Rivera E;Chen JH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- kinetoplastid topoisomerase II;
- kDNA;
- decatenation;
- catenation;
- DNA TOPOISOMERASE;
- LEISHMANIA-INFANTUM;
- TRYPANOSOMA-BRUCEI;
- REPLICATION;
- PARASITES;
- DRUGS;
- DERIVATIVES;
- INHIBITION;
- CLEAVAGE;
- NUCLEAR;
91.Development of pilot scale production process and characterization of a recombinant multiepitope malarial vaccine candidate FALVAC-1A expressed in Escherichia coli

机译：开发中试规模生产工艺并鉴定在大肠杆菌中表达的重组多表位疟疾候选疫苗FALVAC-1A
- 作者：Ravi G;Ella K;Narasu ML
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- Plasmodium falciparum;
- malaria vaccine;
- bioprocess;
- Escherichia coli;
- PLASMODIUM-FALCIPARUM MALARIA;
- RANDOMIZED CONTROLLED-TRIAL;
- MULTISTAGE;
- IMMUNOGENICITY;
- MULTIVALENT;
- EFFICACY;
- CHILDREN;
- DISEASE;
- GENOME;
92.Auto-induction and purification of a Bacillus subtilis transglutaminase (Tgl) and its preliminary crystallographic characterization

机译：枯草芽孢杆菌转谷氨酰胺酶（Tgl）的自动诱导和纯化及其初步晶体学表征
- 作者：Placido D;Fernandes CG;Isidro A;Carrondo MA;Henriques AO;Archer M
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- transglutaminase;
- spore surface;
- spore coat;
- macromolecular assembly;
- cross-linking;
- crystallization;
- BACTERIAL ENDOSPORE COAT;
- SPORE COAT;
- CRYSTAL-STRUCTURE;
- CROSS-LINKING;
- PROTEINS;
- SPORULATION;
- GERMINATION;
- COMPONENT;
- LACCASE;
- LAYERS;
93.Recombinant human epidermal growth factor inclusion body solubilization and refolding at large scale using expanded-bed adsorption chromatography from Escherichia coli

机译：重组人表皮生长因子包涵体的溶解和重折叠，使用大肠杆菌的扩展床吸附色谱法
- 作者：Sharma K;Babu PVC;Sasidhar P;Srinivas VK;Mohan VK;Krishna E
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
94.Expression, purification, and characterization of recombinant human serum albumin fusion protein with two human glucagon-like peptide-1 mutants in Pichia pastoris

机译：带有两个人胰高血糖素样肽-1突变体的重组人血清白蛋白融合蛋白的表达，纯化和鉴定
- 作者：Dou WF;Lei JY;Zhang LF;Xu ZH;Chen Y;Jin J
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- (GLP-1(A2G))(2)-HSA;
- Pichia pastoris;
- purification;
- expression;
- biological half-time;
- GLUCOSE-TOLERANCE;
- INSULIN SENSITIVITY;
- ZUCKER RATS;
- IN-VITRO;
- DEGRADATION;
- POLYPEPTIDE;
- RESISTANT;
- POTENT;
- VIVO;
95.Identification of an alternative translation initiation site for the Pantoea ananatis lycopene cyclase (crt Y) gene in E. coli and its evolutionary conservation

机译：大肠杆菌中泛酸番茄红素番茄红素环化酶（crt Y）基因的替代翻译起始位点的鉴定及其进化保守性
- 作者：Kim SW;Jung WH;Ryu JM;Kim JB;Jang HW;Jo YB;Jung JK;Kim JH
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
96.High yield production of recombinant native and modified peptides exemplified by ligands for G-protein coupled receptors

机译：以G蛋白偶联受体的配体为代表的重组天然和修饰肽的高产量生产
- 作者：Bosse-Doenecke E;Weininger U;Gopalswamy M;Balbach J;Knudsen SM;Rudolph R
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
97.Isotopic labeling of c-type multiheme cytochromes overexpressed in E-coli

机译：大肠杆菌中过度表达的c型多血红素细胞色素的同位素标记
- 作者：Fernandes AP;Couto I;Morgado L;Londer YY;Salgueiro CA
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
98.Characterization and cloning of chitin deacetylases from Rhizopus circinans

机译：圆根霉几丁质脱乙酰基酶的鉴定和克隆
- 作者：Gauthier C;Clerisse F;Dommes J;Jaspar-Versali MF
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- chitin deacetylase;
- enzyme;
- gene expression;
- heterologous expression;
- protein engineering;
- YEAST ASCOSPORE WALL;
- COLLETOTRICHUM-LINDEMUTHIANUM;
- SACCHAROMYCES-CEREVISIAE;
- MUCOR-ROUXII;
- PURIFICATION;
- CHITOSAN;
- EXPRESSION;
- GENE;
- ENZYMES;
99.Heterologous expression of Mytilus californianus foot protein three (Mcfp-3) in Kluyveromyces lactis

机译：乳酸克鲁维酵母中Mytilus californianus足蛋白3（Mcfp-3）的异源表达
- 作者：Platko JD;Deeg M;Thompson V;Al-Hinai Z;Glick H;Pontius K;Colussi P;Taron C;Kaplan DL
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- mussel;
- yeast;
- mcfp-3;
- Mytilus californianus;
- MUSSEL ADHESIVE PROTEIN;
- ESCHERICHIA-COLI;
- EDULIS-L;
- BYSSUS;
100.A new protocol for high-yield purification of recombinant human prothymosin alpha expressed in Escherichia coli for NMR studies

机译：高产率纯化在大肠杆菌中表达的重组人胸腺肽α的新方法用于NMR研究
- 作者：Yi SL;Brickenden A;Choy WY
- 期刊名称：《Protein Expression and Purification》 | 2008年第1期
- prothymosin alpha;
- disordered protein;
- purification;
- heating-cooling extraction;
- ammonium sulfate precipitation;
- ion exchange chromatography;
- isotopic labeling;
- NMR;
- PROTEIN;
- BINDING;
- QUANTITATION;
- CHROMATIN;

上一页
1
2
下一页

客服邮箱：kefu@zhangqiaokeyan.com

客服微信
服务号