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Protein Expression and Purification

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中文名称：蛋白质表达与纯化

ISSN：1046-5928
出版周期：Monthly (except Janu

发文量：4204

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1.Expression of N-formylated proteins in Escherichia coli

机译：N-甲酰化蛋白在大肠杆菌中的表达
- 作者：Spector S.;Flynn JM.;Tidor B.;Baker TA.;Sauer RT.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Protein modification;
- Alpha-amino group;
- Repressor;
- Mutants;
- Dna;
2.Bacterial production and purification of SGPI-1 and SGPI-2, two peptidic serine protease inhibitors from the desert locust, Schistocerca gregaria

机译：来自沙漠蝗虫血吸虫的两种肽丝氨酸蛋白酶抑制剂SGPI-1和SGPI-2的细菌生产和纯化
- 作者：Simonet G.;Claeys I.;Huybrechts J.;De Loof A.;Vanden Broeck J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Fusion protein;
- Insect;
- Trypsin;
- Phenoloxidase;
- Maltose-binding protein;
- Proteinase-inhibitors;
- Dependent expression;
- Migratory locust;
- Insect;
- Cloning;
- Hemolymph;
- Activation;
- Pacifastin;
- Binding;
- Enzyme;
3.DNA gyrase and DNA topoisomerase of Bacillus subtilis: expression and characterization of recombinant enzymes encoded by the gyrA, gyrB and parC, parE genes

机译：枯草芽孢杆菌的DNA促旋酶和DNA拓扑异构酶：由gyrA，gyrB和parC，parE基因编码的重组酶的表达和表征
- 作者：Barnes MH.;LaMarr WA.;Foster KA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Staphylococcus-aureus;
- Escherichia-coli;
- Primary target;
- Iv;
- Fluoroquinolones;
- Purification;
- Replication;
- Quinolones;
- Cleavage;
4.Cytoplasmic expression of the Achromobacter xylosoxidans blue copper nitrite reductase in Escherichia coli and characterisation of the recombinant protein

机译：木氧化无色杆菌蓝色亚硝酸铜还原酶在大肠杆菌中的胞质表达及重组蛋白的表征
- 作者：Ho WH.;Ooi BL.;Jorgensen AM.;Borg L.;Jespersen LL.;Christensen HEM.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
5.Heterologous expression of Rhodococcus opacus L-amino acid oxidase in Streptomyces lividans

机译：卵链霉菌中不透明红球菌L-氨基酸氧化酶的异源表达
- 作者：Geueke B.;Hummel W.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Apoptosis-inducing factor;
- Snake-venom;
- Molecular-cloning;
- Escherichia-coli;
- Apoxin-i;
- Purification;
- Protein;
- Cytotoxicity;
6.Expression of a novel recombinant dual human stem cell factor in insect cells

机译：新型重组双重人类干细胞因子在昆虫细胞中的表达
- 作者：Han JH.;Yan XH.;Zhu J.;Zhi XY.;Zang YH.;Shen BF.;Qin JC.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Dual human stem cell factor;
- Insect expression system;
- Sf9 cell;
- Pacsecg2t;
- Growth-factor;
- Kit-ligand;
- Si-locus;
- Receptor;
7.Cloning, purification, and characterization of thermostable hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis

机译：腾康嗜热厌氧杆菌次生黄嘌呤-鸟嘌呤磷酸核糖基转移酶的克隆，纯化及鉴定
- 作者：Chen Q.;You DL.;Hua MH.;Gu XC.;Luo M.;Lu SY.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
8.Overexpression of juvenile hormone binding protein in bacteria and Pichia pastoris

机译：细菌和巴斯德毕赤酵母中幼稚激素结合蛋白的过表达
- 作者：Grzelak K.;Kludkiewicz B.;Kolomiets LI.;Debski J.;Dadlez M.;Lalik A. Ozyhar A.;Kochman M.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Jhbp;
- Pichia pastoris;
- Recombinant jhbp;
- Galleria mellonella;
- Galleria-mellonella hemolymph;
- Manduca-sexta;
- Ligand-binding;
- Yeast;
- Purification;
- Expression;
- Cloning;
- Cdna;
- Gene;
- Dna;
9.Recombinant expression of Mus musculus myoglobin

机译：小家鼠肌红蛋白的重组表达
- 作者：Bianchi M.;Clementi ME.;Maras B.;Schinina ME.;Bozzi M.;Giardina B. Brancaccio A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Myoglobin;
- Recombinant expression;
- Ligand binding;
- Mass spectrometry;
- Escherichia-coli;
- Nitric-oxide;
- Model system;
- Mouse;
- Hemoglobin;
- Cloning;
- Globin;
- Reconstitution;
- Mutagenesis;
- Binding;
10.Expression, purification, and PC1-mediated processing of (H10D, P28K, and K29P)-human proinsulin

机译：（H10D，P28K和K29P）-人胰岛素原的表达，纯化和PC1介导的加工
- 作者：Mackin RB.;Choquette MH.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Recombinant human proinsulin;
- Prohormone thiol protease;
- Pro-neuropeptide-y;
- Escherichia-coli;
- Nonenzymic reactivation;
- Subtilisin family;
- Insulin;
- Endopeptidase;
- Convertases;
- Pc2;
11.Purification and characterization of the deoxynucleoside monophosphate kinase of bacteriophage T5

机译：T5噬菌体脱氧核苷单磷酸激酶的纯化与鉴定
- 作者：Mikoulinskaia GV.;Gubanov SI.;Zimin AA.;Kolesnikov IV.;Feofanov SA. Miroshnikov AI.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Ump-cmp kinase;
- Escherichia-coli;
- Protein;
- Expression;
- Sequence;
- Cloning;
- Complex;
- Enzyme;
- Phage;
- Genes;
12.Heat elution chromatography of immunoglobulins

机译：免疫球蛋白的热洗脱色谱
- 作者：Osmark P.;Cedervall T.;Pieters K.;Akerstrom B.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Antibodies;
- Purification;
- M proteins;
- Coiled-coil;
- Thermal unfolding;
- Group-a streptococci;
- Gram-positive bacteria;
- Protein-h;
- Surface-proteins;
- Escherichia-coli;
- Binding protein;
- Ig-binding;
- Gene;
- Antibodies;
- Pyogenes;
13.Purification and characterization of a recombinant alpha-N-acetylgalactosaminidase from Clostridium perfringens

机译：产气荚膜梭菌的重组α-N-乙酰半乳糖苷酶的纯化和鉴定
- 作者：Hsieh HY.;Calcutt MJ.;Chapman LF.;Mitra M.;Smith DS.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Alpha-n-acetylgalactosaminidase;
- Abo blood group;
- Seroconversion;
- Blood type a(2) epitope;
- Clostridium perfringens;
- Molecular-cloning;
- Group-b;
- Group-o;
- Group-a;
- Expression;
- Sequence;
- Gene;
- Galactosidase;
- Liver;
- Transfusions;
14.The purification of IgY from chicken egg yolk by preparative electrophoresis

机译：制备型电泳从蛋黄中纯化IgY
- 作者：Gee SC.;Bate IM.;Thomas TM.;Rylatt DB.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Polyclonal antibodies;
- Avoid interference;
- Immunoglobulins;
- Proteins;
- Tool;
15.Purification of Her-2 extracellular domain and identification of its cleavage site

机译：Her-2细胞外结构域的纯化及其切割位点的鉴定
- 作者：Yuan CX.;Lasut AL.;Wynn R.;Neff NT.;Hollis GF.;Ramaker ML.;Rupar MJ. Liu P.;Meade R.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Polyacrylamide-gel electrophoresis;
- Breast-cancer cells;
- Protein;
- Receptor;
- Ectodomain;
16.The solvent in CNBr cleavage reactions determines the fragmentation efficiency of ketosteroid isomerase fusion proteins used in the production of recombinant peptides

机译：CNBr裂解反应中的溶剂决定了重组肽生产中使用的酮固醇异构酶融合蛋白的裂解效率
- 作者：Rodriguez JC.;Wong L.;Jennings PA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
17.Purification of native dehydrin from Glycine Max cv., Pisum sativum, and Rosmarinum officinalis by affinity chromatography

机译：通过亲和色谱法从大豆，豌豆和迷迭香中纯化天然脱水素
- 作者：Herzer S.;Kinealy K.;Asbury R.;Beckett P.;Eriksson K.;Moore P.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Mass-spectrometry;
- Larval hemolymph;
- Alpha-synuclein;
- Protein;
- Peptide;
- Acid;
- Matrix;
- Plants;
- Biotechnology;
- Dehydration;
18.Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris

机译：巴斯德毕赤酵母中单链抗体（scFv）基因表达的最佳条件
- 作者：Shi XZ.;Karkut T.;Chamankhah M.;Alting-Mees M.;Hemmingsen SM.;Hegedus D.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
19.Screening molluscan cDNA expression libraries with anti-shell matrix antibodies

机译：用抗壳基质抗体筛选molluscan cDNA表达文库
- 作者：Marin F.;de Groot K.;Westbroek P.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Organic matrix;
- Proteins;
- Cloning;
- Calcification;
- Allergen;
- Western;
- Layer;
20.Purification and characterization of YihA, an essential GTP-binding protein from Escherichia coli

机译：大肠杆菌的必需GTP结合蛋白YihA的纯化和表征
- 作者：Lehoux IE.;Mazzulla MJ.;Baker A.;Petit CM.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Signal recognition particle;
- Guanine-nucleotide-binding;
- Receptor;
- Domain;
- Gene;
- Ftsy;
21.Expression and characterization of human bifunctional peptidylglycine alpha-amidating monooxygenase

机译：人双功能肽基甘氨酸α-酰胺化单加氧酶的表达和表征
- 作者：Satani M.;Takahashi K.;Sakamoto H.;Harada S.;Kaida Y.;Noguchi M.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
22.Expression of a Brassic napus glutamate 1-semialdehyde aminotransferase in Escherichia coli and characterization of the recombinant protein

机译：甘蓝型油菜谷氨酸1-半醛氨基转移酶在大肠杆菌中的表达及重组蛋白的表征
- 作者：Tsang EWT.;Hu ZY.;Chang Q.;McGregor DI.;Keller WA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
23.One-step purification and refolding of recombinant photoprotein aequorin by immobilized metal-ion affinity chromatography

机译：固定化金属离子亲和色谱法一步纯化和重组重组光蛋白水母发光蛋白
- 作者：Glynou K.;Ioannou PC.;Christopoulos TK.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Aequorin;
- Bioluminescence;
- Immobilized metal ion-affinity chromatography;
- Escherichia-coli;
- Apoaequorin;
- Protein;
- Coelenterazine;
- Regeneration;
- Immunoassay;
- Binding;
- Assays;
- Dna;
24.The first semi-synthetic serine protease made by native chemical ligation

机译：通过天然化学连接制备的第一个半合成丝氨酸蛋白酶
- 作者：Pal G.;Santamaria F.;Kossiakoff AA.;Lu WY.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
25.Production and characterization of the Talaromyces stipitatus feruloyl esterase FAEC in Pichia pastoris: identification of the nucleophilic serine

机译：巴斯德毕赤酵母中Talaromyces stipitatus阿魏酸酯酶FAEC的生产和表征：亲核丝氨酸的鉴定
- 作者：Crepin VF.;Faulds CB.;Connerton IF.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Sugar-beet pulp;
- Aspergillus-niger;
- Acid esterase;
- Clostridium-thermocellum;
- Wheat bran;
- Purification;
- Streptomyces;
- Degradation;
- Cellulose;
- Xylanase;
26.Molecular cloning, overexpression in Escherichia coli, and purification of 6x his-tagged C-terminal domain of Clostridium difficile toxins A and B

机译：分子克隆，在大肠杆菌中的过表达以及艰难梭菌毒素A和B的6x his标记的C末端结构域的纯化
- 作者：Letourneur O.;Ottone S.;Delauzun V.;Bastide MC.;Foussadier A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Prokaryote;
- Expression;
- Clostridium difficile;
- Diagnosis;
- Positive strain;
- Gene;
- Localization;
- Prevalence;
- Expression;
- Antibodies;
- Infection;
- Proteins;
- Disease;
- Mice;
27.Pharmacological characterization and immunoaffinity purification of metabotropic glutamate receptor from Drosophila overexpressed in Sf9 cells

机译：果蝇中过表达的果蝇代谢型谷氨酸受体的药理特性和免疫亲和纯化
- 作者：Panneels V.;Eroglu C.;Cronet P.;Sinning I.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Glutamate;
- Gpcr;
- Baculovirus;
- Overexpression;
- Immunoaffinity purification;
- Integral membrane-proteins;
- Ligand-binding;
- Functional expression;
- Recognition;
- Cloning;
- Domain;
28.Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies

机译：从包涵体大规模生产具有生物活性的大肠杆菌谷氨酰-tRNA还原酶
- 作者：Schauer S.;Luer C.;Moser J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- 1-semialdehyde aminotransferase;
- Chlorophyll biosynthesis;
- Circular-dichroism;
- Protein;
- Expression;
- Enzyme;
- Acid;
29.Mammalian AMP-activated protein kinase: functional, heterotrimeric complexes by co-expression of subunits in Escherichia coli

机译：哺乳动物AMP激活的蛋白激酶：通过在大肠杆菌中共表达亚基来形成功能性异三聚体复合物
- 作者：Neumann D.;Woods A.;Carling D.;Wallimann T.;Schlattner U.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Bacterial expression;
- Polycistronic;
- Protein complex;
- Skeletal-muscle;
- Rat-liver;
- Posttranslational modifications;
- Energy homeostasis;
- Creatine-kinase;
- Gamma-subunit;
- In-vitro;
- Purification;
- Expression;
- Glucose;
30.Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)

机译：甲基营养酵母巴斯德毕赤酵母（Pichia pastoris）作为宿主，用于从马铃薯块茎（Solanum tuberosum）生产ATP-二磷酸水解酶（apyrase）
- 作者：Nourizad N.;Ehn M.;Gharizadeh B.;Hober S.;Nyren P.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Smooth-muscle;
- Ecto-atpase;
- Purification;
- Expression;
- Membrane;
- Cd39;
- Identification;
- Inhibitors;
- Ec-3;
- 6;
- 1;
- 5;
- Enzyme;
31.Expression and purification of the Bacillus anthracis protective antigen domain 4

机译：炭疽芽孢杆菌保护性抗原域4的表达和纯化
- 作者：Krishnanchettiar S.;Sen J.;Caffrey M.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Mammalian-cells;
- Drug discovery;
- Gene synthesis;
- Toxin;
- Receptor;
- Nmr;
- Identification;
- Inhibitors;
- Binding;
32.DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli

机译：DnaK和DnaJ促进了折叠过程并减少了在大肠杆菌中过表达的镁转运蛋白CorA的包涵体形成
- 作者：Chen Y.;Song JM.;Sui SF.;Wang DN.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Magnesium transporter;
- Membrane protein folding;
- Inclusion bodies;
- Chaperones;
- Co-expression;
- Bacterial cytoplasmic membrane;
- Molecular chaperones;
- Atp synthase;
- Proteins;
- Expression;
- Chromatography;
- Insertion;
- Detergent;
- Alpha;
- Hydrophobicity;
33.High yield expression, refolding, and characterization of recombinant interferon alpha 2/alpha 8 hybrids in Escherichia coli

机译：重组干扰素α2 /α8杂种在大肠杆菌中的高产量表达，重折叠和表征
- 作者：Platis D.;Foster GR.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Non-detergent sulfobetaines;
- Affinity purification;
- Crystal-structure;
- Alpha subtypes;
- Cells;
- Proteins;
- Ifn-alpha-8;
- Resolution;
- Evolution;
- Complex;
34.Mistranslational errors associated with the rare arginine codon CGG in Escherichia coli

机译：与大肠杆菌中稀有精氨酸密码子CGG相关的翻译错误
- 作者：McNulty DE.;Claffee BA.;Huddleston MJ.;Kane JF.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- High-level expression;
- Transfer-rna;
- Protein;
- Growth;
- Gene;
- Misincorporation;
- Translation;
- Abundance;
35.A bicistronic expression system for bacterial production of authentic human interleukin-18

机译：双顺反子表达系统，用于细菌生产真正的人白介素-18
- 作者：Kirkpatrick RB.;McDevitt PJ.;Matico RE.;Nwagwu S.;Trulli SH.;Mao J. Moore DD.;Yorke AF.;McLaughlin MM.;Knecht KA.;Elefante LC.;Calamari AS.;Fornwald JA.;Trill JJ.;Jonak ZL.;Kane J.;Patel PS.;Sathe GM. Shatzman AR.;Tapley PM.;Johanson KO.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Gamma-inducing factor;
- Fibroblast-growth-factor;
- N-terminal methionine;
- Escherichia-coli;
- Cysteine protease;
- Crystal-structure;
- Binding-protein;
- Caspase family;
- T-cells;
- Il-18;
36.Isolation of the gene and large-scale expression and purification of recombinant Erythrina cristagalli lectin

机译：重组赤藓促凝集素基因的分离及大规模表达与纯化
- 作者：Stancombe PR.;Alexander FCG.;Ling R.;Matheson MA.;Shone CC.;Chaddock JA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Erythrina cristagalli;
- Erythrina-related lectins;
- Cloning;
- Bacterial expression system;
- Lactose affinity;
- Dorsal-root ganglia;
- Escherichia-coli;
- Corallodendron lectin;
- Agglutinin;
- Proteins;
- Cloning;
- Site;
- Rat;
- Recognition;
- Neurons;
37.Hyperexpression and purification of biologically active human luteinizing hormone and human chorionic gonadotropin using the methylotropic yeast, Pichia pastoris

机译：使用甲基向性酵母巴斯德毕赤酵母过表达和纯化具有生物活性的人黄体生成素和绒毛膜促性腺激素
- 作者：Gadkari R.;Deshpande R.;Dighe RR.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Gonadotropins;
- Glycoprotein hormones;
- Pichia pastoris;
- Recombinant expression;
- Follicle-stimulating-hormone;
- Beta-subunit;
38.Expression of caltrin in the baculovirus system and its purification in high yield and purity by cobalt (II) affinity chromatography

机译：钙网蛋白在杆状病毒系统中的表达及其钴（II）亲和色谱法的高纯度纯化
- 作者：Phan TCA.;Nowak KJ.;Akkari PA.;Zheng MH.;Xu JK.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Caltrin;
- Svsvii;
- Co2+;
- Protein purification;
- Baculovirus;
- Calcium-transport inhibitor;
- One-step purification;
- Seminal-vesicle;
- Guinea-pig;
- Protein;
- Spermatozoa;
- Binding;
- Vectors;
- Plasma;
- Mouse;
39.Heterologous expression in Pichia pastoris and single-step purification of a cysteine proteinase from northern shrimp

机译：毕赤酵母中的异源表达和北部虾半胱氨酸蛋白酶的一步纯化
- 作者：Aoki H.;Ahsan MN.;Watabe S.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Cathepsin l;
- Cysteine proteinase;
- Northern shrimp;
- Overexpression;
- Pichia pastoris;
- Recombinant;
- Plasmid-fusion system;
- Escherichia-coli;
- Low-temperature;
- Methylotrophic yeast;
- Rapid construction;
- Recombinant-dna;
- Cathepsin-l;
- Quantities;
- Proteases;
- Gene;
40.Overexpression and purification of untagged polynucleotide phosphorylases

机译：未标记多核苷酸磷酸化酶的过表达和纯化
- 作者：Jones GH.;Symmons MF.;Hankins JS.;Mackie GA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
41.SwellGel: a sample preparation affinity chromatography technology for high throughput proteomic applications

机译：SwellGel：用于高通量蛋白质组学应用的样品制备亲和色谱技术
- 作者：Haney PJ.;Draveling C.;Durski W.;Romanowich K.;Qoronfleh MW.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Desorption/ionization mass-spectrometry;
- Biotinylated monoclonal-antibodies;
- Protein-g;
- Phosphopeptides;
- Binding;
- Albumin;
- Matrix;
- Phosphoproteome;
- Purification;
- Tool;
42.Cloning and characterization of a gene encoding trehalose phosphorylase (TP) from Pleurotus sajor-caju

机译：侧耳侧耳海藻糖磷酸化酶（TP）基因的克隆与鉴定
- 作者：Han SE.;Kwon HB.;Lee SB.;Yi BY.;Murayama I.;Kitamoto Y.;Byun MO.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
43.A favorable solubility partner for the recombinant expression of streptavidin

机译：链霉亲和素重组表达的良好溶解性伴侣
- 作者：Sorensen HP.;Sperling-Petersen HU.;Mortensen KK.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Fusion protein;
- Streptavidin;
- Insoluble protein;
- Inclusion bodies;
- Solubility tag;
- Maltose-binding protein;
- Initiation-factor if2;
- Escherichia-coli;
- Over-production;
- Systems;
- Surface;
- Fusions;
44.Expression, oxidative refolding, and characterization of six-histidine-tagged recombinant human LECT2, a 16-kDa chemotactic protein with three disulfide bonds

机译：六组氨酸标签的重组人LECT2（具有三个二硫键的16-kDa趋化蛋白）的表达，氧化复性和表征
- 作者：Ito M.;Nagata K.;Kato Y.;Oda Y.;Yamagoe S.;Suzuki K.;Tanokura M.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Escherichia-coli;
- Molecular-cloning;
- Gene;
- Purification;
- Thioredoxin;
- Mim-1;
- Mouse;
45.Isolation of alpha-lactalbumin, beta-lactoglobulin, and bovine serum albumin from cow's milk using gel filtration and anion-exchange chromatography including evaluation of their antigenicity

机译：使用凝胶过滤和阴离子交换色谱法（包括评估其抗原性）从牛奶中分离出α-乳白蛋白，β-乳球蛋白和牛血清白蛋白
- 作者：Neyestani TR.;Djalali M.;Pezeshki M.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Protein purification;
- Alpha-lactalbumin;
- Beta-lactoglobulin;
- Bovine serum albumin;
- Antigenicity;
- Liquid-chromatography;
- Proteins;
- Whey;
- Separation;
- Quantification;
- Purification;
46.Expression and purification of the h1 and h2 isoforms of calponin

机译：钙蛋白的h1和h2亚型的表达和纯化
- 作者：Jin JP.;Wu D.;Gao JM.;Nigam R.;Kwong S.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Smooth-muscle calponin;
- Regulated expression;
- Signal-transduction;
- Troponin-t;
- Molecular-cloning;
- F-actin;
- Protein;
- Cells;
- Inhibition;
- Kinase;
47.Soluble expression of a functionally active Plasmodium falciparum falcipain-2 fused to maltose-binding protein in Escherichia coli

机译：与麦芽糖结合蛋白融合的功能活跃的恶性疟原虫恶性疟原虫2在大肠杆菌中的可溶性表达
- 作者：Goh LL.;Loke P.;Singh M.;Sim TS.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Falcipain-2;
- Expression;
- Solubility;
- Maltose-binding protein;
- Cysteine protease;
- Recombinant falcipain-2;
- Malaria parasite;
- Inhibitors;
- Overexpression;
- Peptide;
- Codons;
- Genes;
48.Molecular cloning, expression, purification, and mass spectrometric characterization of 3C-like protease of SARS coronavirus

机译：SARS冠状病毒3C样蛋白酶的分子克隆，表达，纯化和质谱表征
- 作者：Sun HF.;Luo HB.;Yu CY.;Sun T.;Chen J.;Peng SY.;Qin J.;Shen JH. Yang YM.;Xie YH.;Chen KX.;Wang Y.;Shen X.;Jiang HL.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
49.Expression and purification of the anticomplementary peptide Sh-CRIT-ed1 (formerly Sh-TOR-ed1) as a tetramultimer in Escherichia coli

机译：抗互补肽Sh-CRIT-ed1（以前称为Sh-TOR-ed1）作为四聚体的表达和纯化在大肠杆菌中
- 作者：Oh KS.;Na DK.;Kweon MH.;Sung HC.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Sh-crit;
- Anticomplementary peptide;
- Expression;
- Repetitive artificial peptide and escherichia coli;
- Complement-system;
- Tandem repeats;
- Protein;
- Inhibitors;
- Cleavage;
- Receptor;
- Strategy;
- Mucin;
- Tor;
- C2;
50.Secretion of functional anti-CD30-angiogenin immunotoxins into the supernatant of transfected 293T-cells

机译：功能性抗CD30-血管生成素免疫毒素分泌到转染的293T细胞上清液中
- 作者：Stocker M.;Tur MK.;Sasse S.;Krussmann A.;Barth S.;Engert A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Angiogenin;
- Ki4(scfv);
- Recombinant immunotoxin;
- Eucaryotic expression;
- Hodgkin's lymphoma;
- Immunotherapy;
- Single-chain immunotoxins;
- Escherichia-coli;
- Fusion proteins;
- Cell-line;
- Cancer-therapy;
- Killer-cells;
- Expression;
- Cytotoxicity;
- Angiogenin;
- Lymphoma;
51.Identification and characterization of a second NMN adenylyltransferase gene in Saccharomyces cerevisiae

机译：酿酒酵母中的第二个NMN腺苷酸转移酶基因的鉴定和表征
- 作者：Emanuelli M.;Amici A.;Carnevali F.;Pierella F.;Raffaelli N.;Magni G.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
52.Expression, purification, and characterization of His-tagged human mitochondrial 2,4-dienoyl-CoA reductase

机译：His标记的人类线粒体2，4-二烯酰辅酶A还原酶的表达，纯化和鉴定
- 作者：Chu XS.;Yu WH.;Chen G.;Li D.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Beta-oxidation;
- Unsaturated fatty acids;
- Mitochondria;
- 2;
- 4-dienoyl-coa reductase;
- His-tag;
- Unsaturated fatty-acids;
- Beta-oxidation;
- Liver mitochondria;
- Diabetes-mellitus;
- Dietary-fat;
- Pathway;
- Binding;
- Risk;
53.Efficient isolation, purification, and characterization of the Helicoverpa zea VHDL receptor

机译：高效分离，纯化和表征Helicoverpa zea VHDL受体
- 作者：Persaud DR.;Yousefi V.;Haunerland N.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Density-lipoprotein receptor;
- Storage protein-uptake;
- Fat-body;
- Insect;
54.Cloning and expression of a heme binding protein from the genome of Saccharomyces cerevisiae

机译：酿酒酵母基因组中血红素结合蛋白的克隆与表达
- 作者：Auclair K.;Huang HW.;Moenne-Loccoz P.;de Montellano PRO.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Crystal-structure;
- Iron sources;
- Red alga;
- Oxygenase;
- Gene;
- Biosynthesis;
- Identification;
- Form;
- Replacement;
- Degradation;
55.Production and purification of refolded recombinant human IL-7 from inclusion bodies

机译：从包涵体生产和纯化重折叠的重组人IL-7
- 作者：Ouellette T.;Destrau S.;Zhu JW.;Roach JM.;Coffman JD.;Hecht T.;Lynch JE.;Giardina SL.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Interleukin-7;
- Purification;
- Refolding;
- Chromatography;
- Cytotoxic t-lymphocytes;
- B-lineage cells;
- Growth-factor;
- Human interleukin-7;
- Killer-cells;
- Gamma-chain;
- Receptor;
- Generation;
- Activation;
- Expression;
56.Expression and assembly of Arabidopsis thaliana pyruvate dehydrogenase in insect cell cytoplasm

机译：拟南芥丙酮酸脱氢酶在昆虫细胞质中的表达和装配
- 作者：Szurmak B.;Strokovskaya L.;Mooney BP.;Randall DD.;Miernyk JA.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
57.Production of the bullous pemphigoid antigen 230 (BP230) by Saccharomyces cerevisiae and Pichia pastoris

机译：酿酒酵母和毕赤酵母产生大疱性天疱疮抗原230（BP230）
- 作者：Laffitte E.;Shafaatian R.;Fontao L.;Favre B.;Koster J.;Saurat JH. Monod M.;Borradori L.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Cytoskeleton organization;
- Igg autoantibodies;
- Gene-expression;
- Protein;
- Antibodies;
- Epitopes;
- Dystonin;
- Neurons;
- Domains;
- Sites;
58.Preparation of uniformly labeled NMR samples in Escherichia coli under the tight control of the araBAD promoter: expression of an archaeal homolog of the RNase P Rpp29 protein

机译：在araBAD启动子严格控制下在大肠杆菌中制备均一标记的NMR样品：RNase P Rpp29蛋白的古细菌同源物的表达
- 作者：Boomershine WP.;Raj MLS.;Gopalan V.;Foster MP.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Argu;
- E;
- coli;
- Glycerol minimal medium;
- Isotope labeling;
- Nmr;
- Overexpression;
- Pbad;
- Pet;
- Rnase p;
- T7 rna polymerase;
- High-level expression;
- Arginine transfer-rna;
- Gene-product;
- Cloned genes;
- Codons;
- Argu;
- Translation;
- Polymerase;
- Sequence;
- System;
59.Chromatography of isoforms of recombinant apoaequorin and method for the preparation of aequorin

机译：重组载水母发光蛋白同工型的色谱及其制备方法
- 作者：Masuda H.;Takenaka Y.;Shikamoto Y.;Kagawa M.;Mizuno H.;Tsuji FI.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
60.Production of recombinant CART peptides in Escherichia coli with agonist and antagonist effects on food intake in rats

机译：大肠杆菌中重组CART肽的产生对大鼠食物摄取具有激动剂和拮抗剂作用
- 作者：Couceyro PR.;Fritz T.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Amphetamine-regulated transcript;
- Cocaine;
- Purification;
- Leptin;
61.Human CYP1A1 allelic variants: baculovirus expression and purification, hydrodynamic, spectral, and catalytical properties and their potency in the formation of all-trans-retinoic acid

机译：人CYP1A1等位基因变体：杆状病毒的表达和纯化，流体动力学，光谱和催化特性及其在形成全反式维甲酸中的功效
- 作者：Chernogolov A.;Behlke J.;Schunck WH.;Roots I.;Schwar D.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Cancer susceptibility;
- Nadph-p450 oxidoreductase;
- Metabolism;
- Polymorphisms;
- Genes;
- Benzolt;
- agt;
- pyrene;
- Carcinogenesis;
- Nomenclature;
- Activation;
- Oxidation;
62.Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii

机译：詹氏甲烷球菌甲羟戊酸激酶Arg196和Lys272突变体的表达与纯化
- 作者：Chu XS.;Liu XJ.;Yau M.;Leung YC.;Li D.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
63.Expression of the recombinant antibacterial peptide sarcotoxin IA in Escherichia coli cells

机译：重组抗菌肽肌毒素IA在大肠杆菌细胞中的表达
- 作者：Skosyrev VS.;Kulesskiy EA.;Yakhnin LV.;Temirov YV.;Vinokurov LM.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Peregrina flesh-fly;
- Sarcophaga-peregrina;
- Innate immunity;
- Protein;
- Purification;
64.Construction of a modified vector for efficient purification of recombinant Mycobacterium tuberculosis proteins expressed in Escherichia coli

机译：用于有效纯化在大肠杆菌中表达的重组结核分枝杆菌蛋白的修饰载体的构建
- 作者：Ahmad S.;Ali MM.;Mustafa AS.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Delayed-type hypersensitivity;
- T-cell responses;
- Pe-pgrs proteins;
- Rd1 region gene;
- Immunological diagnosis;
- Antigens;
- Identification;
- Vaccination;
- Mice;
- Cloning;
65.Expression, purification, and characterization of metallothionein-A from rainbow trout

机译：虹鳟鱼金属硫蛋白-A的表达，纯化和鉴定
- 作者：Vergani L.;Grattarola M.;Dondero F.;Viarengo A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Nuclear-magnetic-resonance;
- Oxidative stress;
- Structural characterization;
- 3-dimensional structure;
- I gene;
- Mouse;
- Promoter;
- Spectroscopy;
- Induction;
- Elements;
66.Purification of glucose 6-phosphate dehydrogenase from Buffalo (Bubalus bubalis) erythrocytes and investigation of some kinetic properties

机译：水牛（Bubalus bubalis）红细胞中6-磷酸葡萄糖脱氢酶的纯化及其动力学性质的研究
- 作者：Ciftci M.;Beydemir S.;Yilmaz H.;Altikat S.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Buffalo;
- Purification;
- Glucose 6-phosphate dehydrogenase;
- Erythrocyte;
- Glucose-6-phosphate-dehydrogenase;
67.Purification and characterization of human nucleolar phosphoprotein 140 expressed in Escherichia coli

机译：在大肠杆菌中表达的人核仁磷蛋白140的纯化和鉴定
- 作者：Kim YK.;Jin YN.;Vukoti KM.;Park JK.;Kim EE.;Lee KJ.;Yu YG.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Coiled bodies;
- Nopp140;
- Protein;
- P130;
- Identification;
- Transcription;
- Complexes;
68.Expression of an antitumor-analgesic peptide from the venom of Chinese scorpion Buthus martensii karsch in Escherichia coli

机译：中国蝎子Buthus martensii karsch毒液中抗肿瘤止痛肽在大肠杆菌中的表达
- 作者：Liu YF.;Ma RL.;Wang SL.;Duan ZY.;Zhang JH.;Wu LJ.;Wu CF.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Depressant insect toxin;
- Anti-epilepsy peptide;
- Molecular characterization;
- K+ channels;
- Bmk m1;
- Neurotoxin;
- Purification;
- Crystallization;
- Sequence;
- Cdna;
69.A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process

机译：基于Rubredoxin的系统，用于筛选蛋白表达条件和在线监测纯化过程
- 作者：Kohli BM.;Ostermeier C.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Escherichia-coli;
- Clostridium-pasteurianum;
- Gene;
- Fluorescence;
- Cloning;
- Site;
- Pcr;
- Tag;
70.Expression, purification, and characterization of arginine kinase from the sea cucumber Stichopus japonicus

机译：海参刺参中精氨酸激酶的表达，纯化及鉴定
- 作者：Guo SY.;Guo Z.;Guo Q.;Chen BY.;Wang XC.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Sea cucumber stichopus japonicus;
- Arginine kinase;
- Gene clone;
- Expression;
- Purification;
- Characterization;
- Phosphagen kinase;
- Sequence-analysis;
- Creatine-kinase;
- Evolution;
- Muscle;
- Cdna;
- Gene;
71.Expression and purification of a recombinant avidin with a lowered isoelectric point in Pichia pastoris

机译：等电点降低的重组亲和素在毕赤酵母中的表达和纯化
- 作者：Zocchi A.;Jobe AM.;Neuhaus JM.;Ward TR.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
72.A less laborious approach to the high-throughput production of recombinant proteins in Escherichia coli using 2-liter plastic bottles

机译：使用2升塑料瓶在大肠杆菌中高通量生产重组蛋白的省力方法
- 作者：Millard CS.;Stols L.;Quartey P.;Kim Y.;Dementieva I.;Donnelly MI.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
73.Overexpression, purification, and characterization of recombinant Ca-ATPase regulators for high-resolution solution and solid-state NMR studies

机译：用于高分辨率溶液和固态NMR研究的重组Ca-ATPase调节剂的过表达，纯化和表征
- 作者：Buck B.;Zamoon J.;Kirby TL.;DeSilva TM.;Karim C.;Thomas D.;Veglia G.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
74.Recombinant Yersinia enterocolitica YscM1 and YscM2: homodimer formation and susceptibility to thrombin cleavage

机译：重组小肠结肠炎耶尔森氏菌YscM1和YscM2：同型二聚体的形成和对凝血酶裂解的敏感性
- 作者：Wilharm G.;Neumayer W.;Heesemann J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Low-calcium-response;
- N-terminal domain;
- Iii secretion;
- Polyacrylamide gels;
- Virulence plasmid;
- Gene-expression;
- Proteins;
- Pestis;
- Yoph;
- Dna;
75.Purification and characterization of hexahistidine-tagged elongation factor SelB

机译：六组氨酸标记的延伸因子SelB的纯化与表征
- 作者：Thanbichler M.;Bock A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Selenocysteine insertion-sequence;
- Translation factor selb;
- Messenger-rna;
- Escherichia-coli;
- Selenoprotein synthesis;
- Amino-acid;
- Protein;
- Nucleotides;
- Expression;
- Kinetics;
76.Overexpression of a small medicinal peptide from ginseng in the yeast Pichia pastoris

机译：人参中的一种小药用肽在酵母毕赤酵母中的过表达
- 作者：Yan YJ.;Chen JC.;Li J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Methylotrophic yeast;
- Secretion;
- Expression;
77.Purification and characterization of osteopontin from human milk

机译：人乳中骨桥蛋白的纯化与表征
- 作者：Sorensen S.;Justesen SJ.;Johnsen AH.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Chromatography;
- Crystallization;
- Amino acid sequence;
- Phosphorylation;
- Oxalate crystal-growth;
- Calcium-oxalate;
- Bone sialoprotein;
- Bovine-milk;
- Human urine;
- Crystallization;
- Quantification;
- Uropontin;
- Peptides;
- Proteins;
78.Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry

机译：重组人GM2激活蛋白在昆虫细胞中的表达：质谱的纯化和鉴定
- 作者：Wendeler M.;Lemm T.;Weisgerber J.;Hoernschemeyer J.;Bartelsen O. Schevers U.;Sandhoff K.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- G(m2) activator protein;
- Beta-hexosaminidase-a;
- Degradation;
- Gm2;
- Catabolism;
- Topology;
- Vectors;
79.Purification and folding of recombinant bovine oxoglutarate/malate carrier by immobilized metal-ion affinity chromatography

机译：固定化金属离子亲和色谱法纯化和折叠重组牛磺酸谷氨酸/苹果酸载体
- 作者：Smith VR.;Walker JE.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Integral membrane-proteins;
- Escherichia-coli;
- Bacterial expression;
- Transport proteins;
- Mitochondria;
- Reconstitution;
- Identification;
- Binding;
- Sequence;
- Genes;
80.Expression, purification, and isotope labeling of the Fv of the human HIV-1 neutralizing antibody 447-52D for NMR studies

机译：人类HIV-1中和抗体447-52D Fv的表达，纯化和同位素标记，用于NMR研究
- 作者：Kessler N.;Zvi A.;Ji M.;Sharon M.;Rosen O.;Levy R.;Gorny M. Zolla-Pazner S.;Anglister J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
81.Purification and renaturation of Dictyostelium recombinant alkaline phosphatase by continuous elution electrophoresis

机译：连续洗脱电泳纯化双歧杆菌重组碱性磷酸酶并复性
- 作者：Ubeidat M.;Rutherford CL.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
82.Relationship between self-association of insulin and its secretion efficiency in yeast

机译：胰岛素自我缔合与酵母分泌效率之间的关系
- 作者：Kjeldsen T.;Pettersson AF.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Nuclear-magnetic-resonance;
- Saccharomyces-cerevisiae;
- Intracellular-transport;
- Monomeric insulins;
- Disulfide bonds;
- Quality-control;
- Mutant;
- Stability;
- Pathway;
- Resolution;
83.Cloning, expression, and characterization of a bi-functional disintegrin/alkaline phosphatase hybrid protein

机译：双功能解整合蛋白/碱性磷酸酶杂合蛋白的克隆，表达和表征
- 作者：Butera D.;Skielka K.;McLane MA.;Paquette-Straub C.;Ducancel F.;da Silva AMM.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Disintegrin;
- Eristostatin;
- Alpha(iib)beta(3) integrin;
- Molecular marker;
- Coli alkaline-phosphatase;
- Escherichia-coli;
- Fusion protein;
- Enzyme-immunoassay;
- Integrins;
- Alpha-iib-beta-3;
- Construction;
- Recognition;
- Echistatin;
- Conjugate;
84.Expression and purification of glycine N-methyltransferases in Escherichia coli

机译：甘氨酸N-甲基转移酶在大肠杆菌中的表达和纯化
- 作者：Luka Z.;Wagner C.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Crystal-structure;
- Rat;
- Metabolism;
- Acid;
85.Statistical optimization for immobilized metal affinity purification of secreted human erythropoietin from Drosophila S2 cells

机译：统计优化的固定的金属亲和力从果蝇S2细胞分泌的人类促红细胞生成素的纯化。
- 作者：Shin HS.;Cha HJ.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
86.Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp strain B11-1

机译：嗜冷假单胞菌属菌株B11-1的具有独特一级结构的冷适应酯酶的克隆，异源表达，复性和表征
- 作者：Suzuki T.;Nakayama T.;Choo DW.;Hirano Y.;Kurihara T.;Nishino T. Esaki N.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Escherichia-coli;
- Gene cloning;
- Enzyme-purification;
- Active esterase;
- Lipase;
- Identification;
- Biotechnology;
- Bacterium;
- Proteins;
- Protease;
87.High-level expression of Aliciclobacillus acidocaldarius thioredoxin in Pichia pastoris and Bacillus subtilis

机译：嗜酸乳酸杆菌硫氧还蛋白在巴斯德毕赤酵母和枯草芽孢杆菌中的高表达
- 作者：Anna DF.;Rosa M.;Emilia P.;Simonetta B.;Mose R.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Proteins;
- Stability;
- Cells;
- Genes;
- Acid;
88.Purification of enzymatically active human lysyl oxidase and lysyl oxidase-like protein from Escherichia coli inclusion bodies

机译：从大肠杆菌包涵体中纯化具有酶活性的人赖氨酰氧化酶和类似赖氨酰氧化酶的蛋白
- 作者：Jung ST.;Kim MS.;Seo JY.;Kim HC.;Kim Y.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Amine oxidase;
- Lysyl oxidase;
- Lysyl oxidase-like protein;
- Refolding;
- Cysteine-rich domain;
- Liver fibrosis;
- Gene;
- Copper;
- Acid;
89.Expression, purification, and characterization of an unstable lysozyme mutant in Pichia pastoris

机译：巴斯德毕赤酵母中一个不稳定的溶菌酶突变体的表达，纯化和鉴定
- 作者：Liu ST.;Saito A.;Azakami H.;Kato A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Saccharomyces-cerevisiae;
- Bactericidal action;
- Thermal-stability;
- Protein;
- Secretion;
- Yeast;
- Level;
- Glycosylation;
- Methanol;
- Sequence;
90.Expression, purification, and biochemical characterization of Chk, a soluble protein tyrosine kinase

机译：可溶性蛋白酪氨酸激酶Chk的表达，纯化和生化特性
- 作者：Ayrapetov MK.;Lee S.;Sun G.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Csk homologous kinase;
- Src family kinases;
- Breast-cancer cells;
- Escherichia-coli;
- Atp-binding;
- Site;
- System;
- Cation;
- Gene;
- Autophosphorylation;
91.Functional expression and direct visualization of the human alpha(2B)-adrenergic receptor and alpha(2B)-AR-green fluorescent fusion protein in mammalian cell using Semliki Forest virus vectors

机译：使用Semliki Forest病毒载体在哺乳动物细胞中表达人α（2B）-肾上腺素能受体和α（2B）-AR-绿色荧光融合蛋白
- 作者：Sen S.;Jaakola VP.;Heimo H.;Engstrom M.;Larjomaa P.;Scheinin M. Lundstrom K.;Goldman A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Gpcr;
- Over-expression;
- Localization;
- Cho cells;
- Fusion protein;
- Semliki forest virus;
- Recombinant human alpha(2)-adrenoceptors;
- High-level expression;
- Coupled receptor;
- Adrenergic-receptors;
- Binding;
- System;
- Gene;
- Localization;
- Membrane;
- Quantitation;
92.Expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli

机译：可溶和可活化形式的牛羧肽酶A在大肠杆菌中的表达
- 作者：Seddi R.;Chaix JC.;Puigserver A.;Guo XJ.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Pancreatic preprocarboxypeptidase a1;
- Carboxypeptidase-a;
- Ternary complex;
- Proproteinase-e;
- Cloning;
- Cdna;
- Sequence;
- Protein;
- Binding;
- Pathway;
93.Purification and characterization of Delta 3Trx-1, a splicing variant of human thioredoxin-1 lacking exon 3

机译：缺少外显子3的人类硫氧还蛋白1的剪接变体Delta 3Trx-1的纯化和表征
- 作者：Jimenez A.;Miranda-Vizuete A.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Truncated thioredoxin;
- Mammalian thioredoxin;
- Redox regulation;
- Expression;
- Cells;
- Activation;
- Involvement;
- Peptide;
- Cloning;
- Cancer;
94.The N-terminal stem region of bovine and human beta 1,4-galactosyltransferase I increases the in vitro folding efficiency of their catalytic domain from inclusion bodies

机译：牛和人β1,4-半乳糖基转移酶I的N末端茎区域可提高其包涵体催化结构域的体外折叠效率
- 作者：Boeggeman EE.;Ramakrishnan B.;Qasba PK.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Beta 1;
- 4-galactosyltransferase;
- N-terminus;
- Folding;
- Stem region;
- Escherichia coli;
- Maltose-binding protein;
- Escherichia-coli;
- Crystal-structure;
- Beta-1-4 galactosyltransferase;
- Expression;
- Purification;
- Cdna;
- Solubility;
- Complex;
- Acetylglucosamine;
95.Expression, purification, and inhibition of in vitro proteolysis of human AMPD2 (isoform L) recombinant enzymes

机译：人AMPD2（同工型L）重组酶的体外蛋白水解表达，纯化和抑制
- 作者：Haas AL.;Sabina RL.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Deaminase isoforms;
- Adenylate deaminase;
- Sequence;
- Cloning;
- Domain;
- Gene;
- Identification;
- Protein;
- Binding;
- Muscle;
96.Expression and purification of polyhistidine-tagged rotavirus NSP4 proteins in insect cells

机译：多组氨酸标记的轮状病毒NSP4蛋白在昆虫细胞中的表达和纯化
- 作者：Rodriguez-Diaz J.;Lopez-Andujar P.;Garcia-Diaz A.;Cuenca J.;Montava R. Buesa J.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Rotavirus;
- Nsp4;
- Baculovirus;
- Insect cells;
- Nonstructural glycoprotein;
- Genetic groups;
- Sequence;
- Enterotoxin;
- Responses;
- Particle;
- Model;
- Serum;
- Vp6;
97.Compaction agent clarification of microbial lysates

机译：微生物裂解物的压实剂澄清
- 作者：DeWalt BW.;Murphy JC.;Fox GE.;Willson RC.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Hiv-1 reverse-transcriptase;
- Purification;
- Dna;
- Precipitation;
- Form;
- Rna;
98.One-step purification of Kunitz soybean trypsin inhibitor

机译：一步纯化Kunitz大豆胰蛋白酶抑制剂
- 作者：Duranti M.;Barbiroli A.;Scaraforti A.;Tedeschi G.;Morazzoni P.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Affinity chromatography;
- Hydrolase inhibitor;
- Heat treatment;
- Seed proteins;
- Water extraction;
- Protease inhibitors;
- Proteins;
- Sequence;
- Seeds;
99.Expression and in vitro activation of Manduca sexta prophenoloxidase-activating proteinase-2 precursor (proPAP-2) from baculovirus-infected insect cells

机译：杆状病毒感染昆虫细胞中Mantuca Sexta前酚氧化酶激活蛋白酶-2前体（proPAP-2）的表达和体外激活
- 作者：Ji CY.;Wang Y.;Ross J.;Jiang HB.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期
- Phenoloxidase;
- Melanization;
- Clip domain;
- Serine proteinase;
- Insect immunity;
- Manduca sexta;
- Armigeres subalbatus;
- Immune-responses;
- Serine-protease;
- Hemolymph;
- Microfilariae;
- Melanization;
- Purification;
- Mediators;
- Cloning;
- Enzyme;
100.Isolation of a novel N-acetylglucosamine-specific lectin from fresh sclerotia of the edible mushroom Pleurotus tuber-regium

机译：从食用菌侧耳菇新鲜菌核中分离出新型N-乙酰氨基葡糖特异性凝集素
- 作者：Wang HX.;Ng TB.
- 期刊名称：《Protein Expression and Purification》 | 2003年第2期

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