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首页> 外文期刊>Phytoprotection >Differentiation of entomopathogenic fungus Beauveria bassiana (Ascomycetes: Hypocreales) isolates by PCR-RFLP.
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Differentiation of entomopathogenic fungus Beauveria bassiana (Ascomycetes: Hypocreales) isolates by PCR-RFLP.

机译：PCR-RFLP区分昆虫病原真菌白僵菌（子囊菌：Hypocreales）。

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The entomopathogenic fungus Beauveria bassiana is a promising biological control agent of several insect pests in agriculture. Molecular approaches (PCR, DNA sequence analysis and PCR-RFLP) were used in our research as tools for the identification of different B. bassiana isolates. Our work consisted in identifying the 18S, ITS1, 5.8S, ITS2 and 28S regions of B. bassiana ribosomal DNA. The DNA sequences of the amplified regions showed that the 18S rDNA is the most conserved unit, with a high homology (99.5%) between the isolates studied, while the 3' end of the 28S rDNA has a great variability, which makes it possible to differentiate the isolates. The PCR-RFLP method was used to monitor isolates of B. bassiana and distinguish them in a target pest, Lygus lineolaris. This method involved two main steps. First, PCR was used to amplify a region of the 28S gene of B. bassiana. Second, this PCR product was digested using restriction endonucleases, and the fragments produced were compared using gel electrophoresis. Because of the high specificity and sensitivity of PCR-RFLP, it was possible to discriminate between B. bassiana isolates using spores scraped from the surface of an infected insect as samples.
机译：昆虫病原真菌白僵菌是农业中几种害虫的有希望的生物防治剂。在我们的研究中，分子方法（PCR，DNA序列分析和PCR-RFLP）被用作鉴定不同B的工具。鲈鱼分离株。我们的工作包括确定B的18S，ITS1、5.8S，ITS2和28S区域。鲈鱼核糖体DNA。扩增区域的DNA序列显示18S rDNA是最保守的单元，在所研究的分离株之间具有高度同源性（99.5％），而28S rDNA的3'末端具有很大的可变性，这使得可能区分分离株。 PCR-RFLP方法用于监测B的分离物。鲈鱼，并将其区分为目标害虫 Lygus lineolaris 。此方法涉及两个主要步骤。首先，使用PCR扩增B的28S基因的区域。低音。其次，使用限制性核酸内切酶消化该PCR产物，并使用凝胶电泳比较产生的片段。由于PCR-RFLP的高特异性和敏感性，因此可以区分B。用从被感染昆虫表面刮下的孢子作为样品分离出鲈鱼。

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