• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >REPLICATION REGIONS OF TWO PAIRS OF INCOMPATIBLE LACTOCOCCAL THETA-REPLICATING PLASMIDS
【24h】

REPLICATION REGIONS OF TWO PAIRS OF INCOMPATIBLE LACTOCOCCAL THETA-REPLICATING PLASMIDS

机译：两对不相容的乳球菌θ复制质粒的复制区

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Incompatibility tests were performed employing 12 replicons belonging to a family of homologous lactococcal theta-replicating plasmids. Two pairs of incompatible plasmids were found namely, pFV1001 and pFV1201, and pJW565 and pFW094. The replicons of plasmids pFV1001, pFV1201, pJW565, pJW566, and pFW094 were sequenced. Alignments were made of the replicational origins (repA) and putative replication proteins (RepB) of these and ii related plasmid sequences. Comparison of the alignments with the incompatibility data indicated that the incompatibility determinant could be contained within the 22-bp tandem repeats DRII and/or the inverted repeat IR1 in repA, In support the incompatibility determinant of pJW563 was localized to a 743-bp fragment encompassing repA. A stretch of 13 amino acids of RepB was proposed to be responsible for the plasmid-specific initiation of replication. This stretch is pan, of a domain containing features that are highly conserved within the proposed DNA binding regions of the initiation proteins from several well-characterized plasmids from Gram-negative bacteria, including pSC101, R6K, and mini-F. (C) 1997 Academic Press. [References: 61]
机译：使用属于同源乳球菌θ复制质粒的家族的12个复制子进行不相容性测试。发现了两对不兼容的质粒，分别是pFV1001和pFV1201，以及pJW565和pFW094。对质粒pFV1001，pFV1201，pJW565，pJW566和pFW094的复制子进行测序。比对这些和ii个相关质粒序列的复制起点（repA）和推定复制蛋白（RepB）。比对与不相容性数据的比较表明，不相容性决定簇可以包含在repA的22 bp串联重复序列DRII和/或反向重复序列IR1中。为了支持，pJW563的不相容性决定簇定位于一个743 bp的片段中repA。 RepB的一段13个氨基酸被提议负责质粒特异性复制的起始。此延伸是包含具有高度保守性的结构域的结构域的结构域，该结构域在来自革兰氏阴性细菌的几个充分表征的质粒（包括pSC101，R6K和mini-F）的起始蛋白的拟议DNA结合区内是高度保守的。 （C）1997学术出版社。 [参考：61]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号