首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >ELECTROPORATION-MEDIATED TRANSFORMATION OF ARCANOBACTERIUM (ACTINOMYCES) PYOGENES
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ELECTROPORATION-MEDIATED TRANSFORMATION OF ARCANOBACTERIUM (ACTINOMYCES) PYOGENES

机译:电转化介导的炭疽菌(放线菌)的转化

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Plasmids derived from pNG2 or RSF1010 were introduced into strains of Arcanobacterium (Actinomyces) pyogenes by electroporation. Electroporation conditions were varied systematically to give a maximum electroporation frequency of 3.7 x 10(5) CFU/mu g DNA at 1.5 kV/cm and 246 Omega, resulting in a rime constant of approximately 10 ms. The A. pyogenes transformants expressed plasmid-encoded resistance to chloramphenicol, erythromycin, kanamycin, and streptomycin. The source of incoming DNA affected the growth rate of transformants, but not the electroporation efficiency. This is the first report of genetic transformation of the veterinary pathogen A. pyogenes. (C) 1997 Academic Press. [References: 31]
机译:通过电穿孔将源自pNG2或RSF1010的质粒引入化脓性Arcanobacterium(放线菌)菌株中。系统地改变电穿孔的条件,以在1.7 kV / cm和246Ω的条件下,最大电穿孔频率为3.7 x 10(5)CFU /μgDNA,从而产生约10 ms的冰霜常数。化脓农杆菌转化子表达了对氯霉素,红霉素,卡那霉素和链霉素的质粒编码抗性。传入DNA的来源影响转化体的生长速率,但不影响电穿孔效率。这是对兽病原化脓性链球菌进行遗传转化的第一份报告。 (C)1997学术出版社。 [参考:31]

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