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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >ISOLATION AND CHARACTERIZATION OF A PLASMID FROM LACTOBACILLUS FERMENTUM CONFERRING ERYTHROMYCIN RESISTANCE
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ISOLATION AND CHARACTERIZATION OF A PLASMID FROM LACTOBACILLUS FERMENTUM CONFERRING ERYTHROMYCIN RESISTANCE

机译：赋予红霉素抗性的乳酸乳杆菌发酵菌的分离与鉴定

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Lactobacillus fermentum is a lactic acid bacterial species commonly found in the digestive tracts of pigs and rodents and also present in man. We characterized a 5.7-kb plasmid, pLEM3, conferring erythromycin resistance, which was isolated from a porcine strain of L. fermentum. Plasmid pLEM3 established efficiently in L. fermentum, conferred high-level erythromycin resistance (MIC > 1 mg/ml), and was segregationally stable. A deletion derivative of pLEM3, called pLEM5, was constructed and found to be as genetically stable as the parent. A multiple cloning site was inserted into pLEM5, generating plasmid pLEM7. Nucleotide sequence determination of pLEM5 revealed similarities with known genes. The replicon itself is a member of the pC194 family of rolling circle plasmids. The region responsible for erythromycin resistance was 98.2% identical to the emr gene of conjugative transposon Tn/545. (C) 1997 Academic Press. [References: 36]
机译：发酵乳杆菌是一种乳酸菌，通常存在于猪和啮齿动物的消化道中，也存在于人体内。我们表征了5.7 kb质粒pLEM3，赋予红霉素抗性，该质粒是从发酵乳杆菌的猪株中分离出来的。质粒pLEM3在发酵乳杆菌中有效建立，赋予了高水平的红霉素抗性（MIC> 1 mg / ml），并且是分离稳定的。构建了pLEM3的缺失衍生物，称为pLEM5，发现其遗传稳定性与亲本一样。将多个克隆位点插入pLEM5，产生质粒pLEM7。 pLEM5的核苷酸序列测定揭示了与已知基因的相似性。复制子本身是滚环质粒pC194家族的成员。引起红霉素抗性的区域与缀合转座子Tn / 545的emr基因相同，为98.2％。 （C）1997学术出版社。 [参考：36]

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