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首页> 外文期刊>The European Journal of Neuroscience >Identified spinal motoneurons of young rats possess nicotinic acetylcholine receptors of the heteromeric family.
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Identified spinal motoneurons of young rats possess nicotinic acetylcholine receptors of the heteromeric family.

机译：鉴定出的年轻大鼠脊髓运动神经元具有异聚家族的烟碱乙酰胆碱受体。

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Abstract The aim of the present study was to determine whether, in young rats, spinal motoneurons possess functional nicotinic acetylcholine receptors. Motoneurons were identified either by retrograde labelling or by choline acetyltransferase immunohistochemistry. Whole-cell recordings were performed in spinal cord slices cut at the lumbar level. In voltage clamp, acetylcholine evoked a rapidly activating inward current. In current clamp, it depolarized the motoneuron membrane and induced action potential firing. The acetylcholine-evoked current was strongly reduced by d-tubocurarine or dihydro-beta-erythroidine, broad spectrum nicotinic antagonists, but was almost insensitive to methyllycaconitine, a nicotinic antagonist selective for receptors containing the alpha7 subunit. Moreover, exo-2-(2-pyridyl)-7-azabicyclo[2.2.1]heptane, an alpha7-specific agonist, was without effect. In young animals, light-microscopic autoradiography showed that in the central grey matter all laminae were intensely and equally labelled by [(3)H]epibatidine. A dense [(125)I]-alpha-bungarotoxin binding was also found in all laminae, with slightly lower levels in the superficial layers of the dorsal horns and in the ventral part of the grey matter. In adults, the density of [(3)H]epibatidine binding sites was much lower in the entire grey matter, except in layer 2 of the dorsal horn, and [(125)I]-alpha-bungarotoxin binding sites were present only in some selected areas. Our data indicate that spinal motoneurons possess functional nicotinic receptors of the heteromeric type and suggest that nicotinic cholinergic transmission may play a significant role in the developing spinal cord.
机译：摘要本研究的目的是确定在幼年大鼠中，脊髓运动神经元是否具有功能性的烟碱型乙酰胆碱受体。通过逆行标记或胆碱乙酰基转移酶免疫组织化学鉴定了动子素。在腰水平切开的脊髓切片中进行全细胞记录。在电压钳中，乙酰胆碱引起快速激活的内向电流。在电流钳中，它使运动神经元膜去极化并引起动作电位激发。乙酰胆碱诱发的电流被广谱烟碱拮抗剂d-微管尿嘧啶或二氢-β-赤藓类素强烈降低，但对甲基lycaconitine几乎不敏感，甲基烟碱是一种对含有α7亚基的受体具有选择性的烟碱拮抗剂。此外，exo-2-（2-吡啶基）-7-氮杂双环[2.2.1]庚烷，一种α7特异性激动剂，没有作用。在幼小动物中，光镜放射自显影显示在中央灰质中，所有薄片均被[（3）H] epibatidine强烈且均等地标记。在所有薄片中也发现了密集的[（125）I]-α-真菌毒素结合，在背角的浅层和灰质的腹侧部分的水平较低。在成年人中，[（3）H] epibatidine结合位点的密度在整个灰质中要低得多，除了在背角的第2层，并且[（125）I]-α-邦格鲁毒素结合位点仅在一些选定的区域。我们的数据表明，脊髓运动神经元具有异源类型的功能性烟碱样受体，并表明烟碱胆碱能传递可能在发育中的脊髓中起重要作用。

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