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首页> 外文期刊>The FEBS journal >Engineering stereoselectivity of ThDP-dependent enzymes
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Engineering stereoselectivity of ThDP-dependent enzymes

机译：ThDP依赖酶的工程立体选择性

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摘要

Thiamine diphosphate-dependent enzymes are broadly distributed in allorganisms, and they catalyse a broad range of C–C bond forming andbreaking reactions. Enzymes belonging to the structural families ofdecarboxylases and transketolases have been particularly well investigatedconcerning their substrate range, mechanism of stereoselective carboligationand carbolyase reaction. Both structurally different enzyme familiesdiffer also in stereoselectivity: enzymes from the decarboxylase family arepredominantly R-selective, whereas those from the transketolase family areS-selective. In recent years a key focus of our studies has been on stereoselectivebenzoin condensation-like 1,2-additions. Meanwhile, several S-selectivevariants of pyruvate decarboxylase, benzoylformate decarboxylase and2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC)synthase as well as R-selective transketolase variants were createdthat allow access to a broad range of enantiocomplementary a-hydroxyketonesand a,a′-dihydroxyketones. This review covers recent studies andthe mechanistic understanding of stereoselective C–C bond formingthiamine diphosphate-dependent enzymes, which has been guided by structure–function analyses based on mutagenesis studies and from influences ofdifferent substrates and organic co-solvents on stereoselectivity.
机译：硫胺素二磷酸依赖性酶广泛分布于所有生物中，它们催化广泛的C–C键形成和断裂反应。关于脱羧酶和转酮酶的结构家族的酶，关于它们的底物范围，立体选择性碳连接反应的机理和羧化酶反应的研究特别深入。两种结构不同的酶家族的立体选择性也不同：脱羧酶家族的酶主要是R选择性的，而转酮醇酶家族的酶则是S选择性的。近年来，我们研究的重点是立体选择性安息香缩合样1,2加成。同时，创建了丙酮酸脱羧酶，苯甲酰甲酸酯脱羧酶和2-琥珀酰-5-烯醇丙酮酸6-6-羟基-3-环己烯-1-羧酸（SEPHCHC）合酶的几种S-选择变体以及R-选择性转酮醇酶变体对映体互补的α-羟基酮和a，a'-二羟基酮这篇综述涵盖了对立体选择性C–C键形成硫胺素二磷酸依赖性酶的最新研究和机理理解，该方法已基于基于诱变研究的结构功能分析以及不同底物和有机助溶剂对立体选择性的影响而得到指导。

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