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首页> 外文期刊>Protoplasma: An International Journal of Cell Biology >Super-resolution localization microscopy with photoactivatable fluorescent marker proteins
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Super-resolution localization microscopy with photoactivatable fluorescent marker proteins

机译:具有光活化荧光标记蛋白的超高分辨率定位显微镜

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摘要

Fluorescent proteins (FPs) have become popular imaging tools because of their high specificity, minimal invasive labeling and allowing visualization of proteins and structures inside living organisms. FPs are genetically encoded and expressed in living cells, therefore, labeling involves minimal effort in comparison to approaches involving synthetic dyes. Photoactivatable FPs (paFPs) comprise a subclass of FPs that can change their absorption/ emission properties such as brightness and color upon irradiation. This methodology has found a broad range of applications in the life sciences, especially in localizationbased super-resolution microscopy of cells, tissues and even entire organisms. In this review, we discuss recent developments and applications of paFPs in super-resolution localization imaging.
机译:荧光蛋白(FPs)由于其高特异性,最小的侵入性标记并允许可视化生物体内的蛋白质和结构而成为流行的成像工具。 FPs是通过基因编码并在活细胞中表达的,因此,与涉及合成染料的方法相比,标记涉及的工作量最小。可光活化的FP(paFP)包含FP的一个子类,这些FP可以在照射时改变其吸收/发射特性,例如亮度和颜色。这种方法已经在生命科学中找到了广泛的应用,尤其是在基于定位的细胞,组织甚至整个生物的超分辨率显微镜中。在这篇综述中,我们讨论了paFPs在超分辨率定位成像中的最新发展和应用。

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