A rapid analytical method for free choline by LC and its application for bacterial culture medium samples

Kuribayashi M; Tsuzuki M; Sato K; Abo M; Yoshimura E

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A rapid analytical method for free choline by LC and its application for bacterial culture medium samples

机译：液相色谱法快速分析游离胆碱及其在细菌培养基中的应用

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A rapid, and inexpensive analytical method for free choline is described here. Choline was derivatized with benzoyl chloride and analyzed by HPLC with UV detection. Choline samples in the micro-molar range were determined with good repeatability. The precision (RSD) was not greater than 10%. The quantification limit was 10 mu M (3 sigma), and the linear calibration range was between 10 and 1,500 mu M. This method was applied to the measurement of free choline in a culture medium of bacteria and was shown to be suitable for aqueous samples with a wide concentration range and for routine analyses.

机译：本文介绍了一种快速，廉价的游离胆碱分析方法。胆碱用苯甲酰氯衍生化，并通过HPLC和UV检测进行分析。测定的微摩尔范围内的胆碱样品具有良好的重复性。精度（RSD）不大于10％。定量限为10μM（3 sigma），线性校准范围为10至1,500μM。该方法用于测量细菌培养基中的游离胆碱，已证明适用于水性样品具有广泛的浓度范围，可用于常规分析。

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来源
《Chromatographia》 |2008年第4期|共3页
作者
Kuribayashi M; Tsuzuki M; Sato K; Abo M; Yoshimura E;
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原文格式 PDF
正文语种 eng
中图分类分析化学;
关键词
column liquid chromatography; choline; salt stress; MASS-SPECTROMETRY; GLYCINE BETAINE; CHROMATOGRAPHY; ACETYLCHOLINE; GENES;

机译：柱液相色谱胆碱盐胁迫质谱法甘氨酸丁氨酸色谱乙酰胆碱GENES;

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1. A rapid analytical method for free choline by LC and its application for bacterial culture medium samples [J] . Kuribayashi M, Tsuzuki M, Sato K, Chromatographia . 2008,第3a4期

机译：液相色谱法快速分析游离胆碱及其在细菌培养基中的应用
2. A rapid LC-ESI-MS/MS method for the quantitation of choline, an active metabolite of citicoline: Application to in vivo pharmacokinetic and bioequivalence study in Indian healthy male volunteers [J] . SarkarA.K., GhoshD., HaldarD., Journal of Pharmaceutical and Biomedical Analysis: An International Journal on All Drug-Related Topics in Pharmaceutical, Biomedical and Clinical Analysis . 2012,第Null期

机译：快速的LC-ESI-MS / MS方法定量胆碱（胞磷胆碱的活性代谢产物）：在印度健康男性志愿者体内药代动力学和生物等效性研究中的应用
3. Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples [J] . Shui-lian Bi, Lei Shi, He Yan, Annals of microbiology . 2013,第1期

机译：比较用于分离弯曲杆菌属的各种培养方法（Skirrow培养基，无血培养基和富含Bolton和Preston肉汤的过滤系统）。从生肉样品中
4. Determination of Free Choline, Free Carnitine, and Myo-inositol by LC-MS/MS. [C] . Jonathan R. Draher, Nicholas A. Cellar, Todime M. Reddy ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：通过LC-MS / MS测定游离胆碱，游离肉碱和肌醇肌醇。
5. The utility of MS, LC and LC-MS analytical methods to study some important biological macromolecules and small molecules. [D] . Yang, Mei. 2005

机译：MS，LC和LC-MS分析方法可用于研究一些重要的生物大分子和小分子。
6. Development and bioanalytical method validation of an LC-MS/MS assay for simultaneous quantitation of 2-alkyl-4(1H)-quinolones for application in bacterial cell culture and lung tissue [O] . Luke K. Brewer, Jace W. Jones, Catherine B. Blackwood, -1

机译：用于同时定量2-烷基-4（1H）-喹诺酮的LC-MS / MS测定法的开发和生物分析方法验证可用于细菌细胞培养和肺组织
7. Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: The effect of mixed cultures and sample dilution on bacterial identification [O] . Rehse Steven J., Mohaidat Q.I., Palchaudhuri S. 2010

机译：激光诱导击穿光谱技术在快速病原体诊断中的临床应用：混合培养和样品稀释对细菌鉴定的影响

A rapid analytical method for free choline by LC and its application for bacterial culture medium samples

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