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首页> 外文期刊>The Journal of Nutritional Biochemistry >Eicosapentaenoic acid decreases expression of anandamide synthesis enzyme and cannabinoid receptor 2 in osteoblast-like cells
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Eicosapentaenoic acid decreases expression of anandamide synthesis enzyme and cannabinoid receptor 2 in osteoblast-like cells

机译：二十碳五烯酸减少成骨样细胞中anandamide合成酶和大麻素受体2的表达

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摘要

Anandamide (AEA) is an endogenous agonist for the cannabinoid receptor 2 (CB2) which is expressed in osteoblasts. Arachidonic acid (AA) is the precursor for AEA and dietary n-3 polyunsaturated fatty acids (PUFA) are known to reduce the concentrations of AA in tissues and cells. Therefore, we hypothesized that n-3 PUFA, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DNA), which reduce AA in cells, could lower AEA in osteoblasts by altering enzyme expression of the endocannabinoid (EC) system. MC3T3-E1 osteoblast-like cells were grown for 6, 10, 15, 20, 25 or 30 days in osteogenic medium. Osteoblasts were treated with 10 mu M of AA, EPA, DHA, oleic acid (OA) or EPA+DHA (5 mu M each) for 72 h prior to their collection for measurement of mRNA and alkaline phosphatase (ALP) activity. Compared to vehicle control, osteoblasts treated with AA had higher levels of AA and n-6 PUFA while those treated with EPA and DHA had lower n-6 but higher n-3 PUFA. Independent of the fatty acid treatments, osteoblasts matured normally as evidenced by ALP activity. N-acyl phosphatidylethanolamine-selective phospholipase D (NAPE-PLD), fatty acid amide hydrolase (FAAH) and CB2 mRNA expression were higher at 20 days compared to 10 days. NAPE-PLD and CB2 mRNA was lower in osteoblasts treated with EPA compared to all other groups. Thus, mRNA expression for NAPE-PLD, FAAH, and CB2 increased during osteoblast maturation and EPA reduced mRNA for NAPE-PLD and CB2 receptor. In conclusion, EPA lowered mRNA levels for proteins of the EC system and mRNA for AEA synthesis/degradation is reported in osteoblasts
机译：Anandamide（AEA）是在成骨细胞中表达的大麻素受体2（CB2）的内源性激动剂。花生四烯酸（AA）是AEA的前体，已知饮食中的n-3多不饱和脂肪酸（PUFA）会降低组织和细胞中AA的浓度。因此，我们假设降低细胞中AA的n-3 PUFA，二十碳五烯酸（EPA）和二十二碳六烯酸（DNA）可以通过改变内源性大麻素（EC）系统的酶表达来降低成骨细胞的AEA。 MC3T3-E1成骨细胞样细胞在成骨培养基中生长6、10、15、20、25或30天。在收集成骨细胞之前，先用10μM的AA，EPA，DHA，油酸（OA）或EPA + DHA（各5μM）处理72小时，然后再测量其成骨细胞的mRNA和碱性磷酸酶（ALP）活性。与媒介物对照相比，AA处理的成骨细胞具有较高的AA和n-6 PUFA水平，而EPA和DHA处理的成骨细胞具有较低的n-6但较高的n-3 PUFA。独立于脂肪酸治疗，成骨细胞正常成熟，如ALP活性所证明。在20天时，N酰基磷脂酰乙醇胺选择性磷脂酶D（NAPE-PLD），脂肪酸酰胺水解酶（FAAH）和CB2 mRNA表达高于10天。与所有其他组相比，用EPA处理的成骨细胞中的NAPE-PLD和CB2 mRNA较低。因此，成骨细胞成熟期间，NAPE-PLD，FAAH和CB2的mRNA表达增加，而EPA减少了NAPE-PLD和CB2受体的mRNA表达。总之，据报道，成骨细胞中EPA降低了EC系统蛋白质的mRNA水平，而AEA合成/降解的mRNA有所降低。

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