• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The journal of sexual medicine >The Neuroprotective Effect of Platelet-rich Plasma on Erectile Function in Bilateral Cavernous Nerve Injury Rat Model
【24h】

The Neuroprotective Effect of Platelet-rich Plasma on Erectile Function in Bilateral Cavernous Nerve Injury Rat Model

机译：富血小板血浆对双侧海绵体神经损伤大鼠模型勃起功能的神经保护作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction. Neurogenic erectile dysfunction resulting from cavernous nerve (CN) injury is a major complication caused by radical prostatectomy. The use of platelet-rich plasma (PRP) on the nerve-injured site has shown promising results for the nerve regeneration. However, the effects of PRP injection in corpus cavernosum after bilateral CN injury have never been investigated. Aim. To assess the neuroprotective effect of PRP injection in corpus cavernosum after bilateral CN injury. Methods. Male Sprague-Dawley rats were randomly divided into three groups: Group I underwent sham operation, while the remaining two groups underwent bilateral CN crush. Crush injury groups were treated at the time of injury with an application of PRP or normal saline only injection in the corpus cavernosum, respectively. Four weeks later, erectile function (EF) was assessed by CN electrosimulation, and CNs as well as penile tissue were collected for histology. Main Outcome Measures. Intracavernous pressure (ICP) monitored during electrical stimulation of CNs; myelinated axons number of CNs and dorsal penile nerve; collagen type change, number of apoptotic cells, and mRNA expression of caspase-3 and transforming growth factor-β1 (TGF-β1) in the corpus cavernosum. Results. Four weeks after surgery, in the vehicle-only group, the functional evaluation showed a lower mean maximal ICP than that in the sham group (P<0.05). PRP treatments resulted in significant recovery of EF, as compared with the vehicle-only group (P<0.05). Histologically, the PRP-treated group had a significant preservation of myelinated axons of CNs compared with the vehicle-only group (P<0.05) and reduced the apoptotic index. The mRNA expression of TGF-β1 in the corpus cavernosum tissue was significantly decreased in the PRP group compared with the vehicle-only group (P<0.05). Conclusions. PRP injection in the corpus cavernosum increased the number of myelinated axons and facilitated recovery of EF in the bilateral CN injury rat model.
机译：介绍。海绵状神经（CN）损伤引起的神经源性勃起功能障碍是前列腺癌根治术引起的主要并发症。在神经损伤部位使用富含血小板的血浆（PRP）已显示出令人鼓舞的神经再生结果。然而，从未研究过双侧CN损伤后海绵体中PRP注射的作用。目标。评估PRP注射对双侧CN损伤后海绵体的神经保护作用。方法。将雄性Sprague-Dawley大鼠随机分为三组：第一组进行假手术，其余两组进行双侧CN挤压。挤压伤组在受伤时分别在海绵体中应用PRP或仅注射生理盐水。四周后，通过CN电模拟评估勃起功能（EF），并收集CN和阴茎组织进行组织学检查。主要观察指标。 CNs电刺激过程中监测的腔内压力（ICP）; CNs和阴茎背神经的髓鞘轴突数目;海绵体中胶原蛋白类型的变化，凋亡细胞的数量以及caspase-3和转化生长因子β1（TGF-β1）的mRNA表达。结果。手术后四周，纯载剂组的功能最大ICP较假手术组低（P <0.05）。与单纯赋形剂组相比，PRP治疗可导致EF显着恢复（P <0.05）。从组织学上讲，与单纯媒介物治疗组相比，PRP治疗组具有显着的CNs髓鞘轴突保存（P <0.05），并且凋亡指数降低。与单纯运载体组相比，PRP组海绵体组织中TGF-β1的mRNA表达明显降低（P <0.05）。结论。在双侧CN损伤大鼠模型中，海绵体中的PRP注射增加了髓鞘轴突的数量，并促进了EF的恢复。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号