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首页> 外文期刊>The New Phytologist >PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis
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PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis

机译：PFT1是转录介体复合物亚基，通过拟南芥中的活性氧（ROS）分布控制根毛的分化

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摘要

Root hair morphogenesis is driven by an amalgam of interacting processes controlled by complex signaling events. Redox processes and transcriptional control are critical for root hair development. However, the molecular mechanisms that integrate redoxstate and transcription are largely unknown. To elucidate a possible role of transcriptional Mediators in root hair formation, we analyzed the Arabidopsis root hair phenotype of T-DNA insertion lines that harbor homozygous mutations in genes encoding Mediator subunits. Genetic evidence indicates that the Mediator subunits PFT1/MED25 and MED8 are critical for root hair differentiation, but act via separate mechanisms. Transcriptional profiling of pft1 roots revealed that PFT1 activates a subset of hydrogen peroxide (H202)-producing class III peroxidases. pft1 mutants showed perturbed H202 and superoxide (Or) distribution, suggesting that PFT1 is essential to maintain redox homeostasis in the root. Chemical treatments rescued the pft1 mutant phenotype,indicating that correct reactive oxygen species (ROS) distribution is an essential prerequisite for root hair differentiation. In addition, PFT1 positively regulates cell wall remodeling genes that are essential for root hair formation. Our results demonstrate that PFT1 maintains ROS distribution which, in turn, controls root hair differentiation. Thus, our findings reveal a novel mechanism in which the Mediator controls ROS homeostasis by regulating the transcriptional machinery.
机译：根毛形态发生是由复杂的信号事件控制的相互作用过程的汞齐驱动的。氧化还原过程和转录控制对于根毛的发育至关重要。但是，整合氧化还原状态和转录的分子机制在很大程度上尚不清楚。为了阐明转录介体在根毛形成中的可能作用，我们分析了在编码介体亚基的基因中具有纯合突变的T-DNA插入系的拟南芥根毛表型。遗传证据表明，介体亚基PFT1 / MED25和MED8对根毛分化至关重要，但通过不同的机制起作用。 pft1根的转录谱分析表明PFT1激活了过氧化氢（H202）产生的III类过氧化物酶的一个子集。 pft1突变体表现出扰动的H2O2和超氧化物（Or）分布，表明PFT1对于维持根中的氧化还原稳态是必不可少的。化学处理挽救了pft1突变表型，表明正确的活性氧（ROS）分布是根毛分化的必要先决条件。此外，PFT1积极调节细胞壁重塑基因，这对于根毛的形成至关重要。我们的结果表明PFT1保持ROS分布，进而控制根毛分化。因此，我们的发现揭示了一种新的机制，其中介体通过调节转录机制来控制ROS稳态。

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