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首页> 外文期刊>The Philippine Agricultural Scientist >Efficient In Vitro Culture Protocol for Mass Propagation of Stevia (Stevia rebaudiana Bertoni) in the Philippines
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Efficient In Vitro Culture Protocol for Mass Propagation of Stevia (Stevia rebaudiana Bertoni) in the Philippines

机译：在菲律宾大规模传播甜叶菊（Stevia rebaudiana Bertoni）的高效体外培养方案

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摘要

Studies were conducted to develop an efficient in vitro culture protocol for mass propagation of stevia (Stevie rebaudiana Bertoni) using nodal explants and Murashige and Skoog's (MS) medium formulation supplemented with different types and concentrations of plant growth regulators. The two-step sterilization procedure, which involved the soaking of explants in 1.0% (v/v) sodium hypochlorite for 10 min followed by re-sterilization of contaminated cultures in 0.01% (w/v) mercuric chloride for 1 min, proved to be effective in producing the initial sterile explants of stevia. The MS medium supplemented with 1.0 mg L-1 6-benzylaminopurine (BAP) and 0.1 mg L-1 indoleacetic acid (IAA) was found optimum for callus formation. Greatest shoot proliferation was observed in medium with 5 mg L-1 BAP + 0.1 mg L-1 IAA, with 10 shoots produced per inoculum after 4 wk of culture. Increased elongation of shoots was attained when the microshoots were incubated in hormone-less MS medium before rooting. Regenerated shoots were successfully rooted on half-strength MS medium supplemented with 1.0 mg L-1 naphthaleneacetic acid.
机译：进行了研究，以开发一种有效的体外培养方案，用于使用节点外植体以及补充了不同类型和浓度的植物生长调节剂的Murashige和Skoog（MS）培养基配方对甜叶菊（Stevie rebaudiana Bertoni）进行大量繁殖。分两步进行的灭菌程序包括：将外植体在1.0％（v / v）的次氯酸钠中浸泡10分钟，然后将污染的培养物在0.01％（w / v）的氯化汞中再灭菌1分钟，这证明了能有效地产生甜叶菊的最初无菌外植体。发现补充有1.0 mg L-1 6-苄基氨基嘌呤（BAP）和0.1 mg L-1吲哚乙酸（IAA）的MS培养基最适合形成愈伤组织。在含有5 mg L-1 BAP + 0.1 mg L-1 IAA的培养基中观察到最大的芽增殖，培养4周后每个接种体产生10芽。当微枝在生根前在无激素的MS培养基中孵育时，枝条的伸长增加。再生的芽成功地生根于补充有1.0 mg L-1萘乙酸的半强度MS培养基上。

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