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首页> 外文期刊>Advancements in Life Sciences >Protocol optimization for efficient in vitro micro-propagation of Stevia rebaudiana Bertoni
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Protocol optimization for efficient in vitro micro-propagation of Stevia rebaudiana Bertoni

机译：高效优化甜叶菊甜叶菊体外微繁殖的方案优化

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Background: Stevia rebaudiana Bertoni is one of the most amazing herb that is an abundant source of diterpene glycoside a non-caloric sweetener. In recent years alongside its utilization in herbal and folk medicine, commercial exploitation of this herb for weight control and anti-diabetic treatment has started. Methods: In present study healthy nodal segments of Stevia rebaudiana Bertoni were used as explants for optimization of in vitro micro-propagation and regeneration protocol for rapid, fast and efficient multiplication under in-vitro conditions . For shoot proliferation and rooting various concentrations cytokinins NAA (Naphthalene acetic acid), BAP (Benzyl amino purine), IAA (Indole acetic acid) and kinetin (Kn) were employed. Results: Shoots without roots were regenerated from nodal segments via auxiliary shoot induction. The production of multiple shoots from nodal portion was maximum on MS medium complemented with 0.5 mg/L of BAP and 1.5 mg/L of Kn. Maximum rooting was recorded on MS medium supplemented with 0.25 mg/L NAA and 1.0 mg/L of IAA. Successful hardening was achieved under controlled growth room conditions followed by transfer to glass house and open field. Conclusion: Standardization of explant source and culturing media for multiplication of shoot resulted in mass proliferation of homogenous and healthy plantlets of Stevia clones under local conditions.
机译：背景：甜叶菊甜叶菊是最令人惊奇的草药之一，它是非热量甜味剂二萜糖苷的丰富来源。近年来，除了在草药和民间医学中的应用外，这种草药用于体重控制和抗糖尿病治疗的商业开发已经开始。方法：在本研究中，将甜叶甜叶菊的健康节段用作外植体，以优化体外微繁殖和再生方案，以在体外条件下快速，快速和有效地繁殖。为了使芽增殖和生根，使用了各种浓度的细胞分裂素NAA（萘乙酸），BAP（苄基氨基嘌呤），IAA（吲哚乙酸）和激动素（Kn）。结果：无根的芽通过辅助芽诱导从节节中再生。在补充有0.5 mg / L BAP和1.5 mg / L Kn的MS培养基上，从淋巴结部分产生的多枝芽最大。在补充有0.25 mg / L NAA和1.0 mg / L IAA的MS培养基上记录最大生根。在受控的生长室条件下成功实现了硬化，然后转移到温室和露天场地。结论：标准化的外植体来源和培养基用于枝条繁殖导致甜叶菊无性系同质和健康苗在局部条件下大量繁殖。

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