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首页> 外文期刊>Indian Journal of Experimental Biology >A rapid and efficient protocol for in vitro multiplication of genetically uniform Stevia rebaudiana (Bertoni)
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A rapid and efficient protocol for in vitro multiplication of genetically uniform Stevia rebaudiana (Bertoni)

机译：一种快速有效的方法，用于在体外繁殖遗传上一致的甜叶菊（Bertoni）

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摘要

Stevia rebaudiana (Bertoni), commonly called candy leaf or sweet leaf, endemic to South America, is an important medicinal plant. As a source of low calorie natural sweetener 'stevoside', it is used in obesity, diabetes, treatment of heartburn and tooth decay, and also serves as a food supplement. Large scale commercial propagation of S. rebaudiana demands a suitable protocol. Here, we propose an improved protocol for in vitro multiplication of S. rebaudiana from nodal explants. In this protocol, the effect of laboratory grade urea on multiple shoot induction from nodal explants was studied. The nodal explants were initially cultured on Murashige and Skoog (MS) basal media for 2 weeks which facilitated the axillary bud break. Further, culturing of these explants on MS medium fortified with 6 benzyl aminopurine (BAP) (2 mg/L) and Naphthalene acetic acid (NAA) (1 mg/L) with and-mahout urea (5 mg/L) for a period of 40 days revealed maximum shoot production of 44.56 from a single nodal explant in media supplemented with urea as compared to 22.44 without urea. The differences in the number of shoots produced were significant and these shoots readily rooted in MS media with NAA (4 mg/L). Primary and secondary hardening was successful in these plants. There were no visible morphological abnormalities observed in the micropropagated plantlets. Genetic analysis from random samples also revealed that these plants are genetically uniform. The advantage of the present protocol is that the complete process of multiple shoot induction, rooting and hardening could be completed within a period of 6 months as compared to the existing protocols.
机译：甜叶菊（Bertoni），通常被称为糖果叶或甜叶，在南美洲特有，是一种重要的药用植物。作为低热量天然甜味剂“甜菊糖”的来源，它可用于肥胖症，糖尿病，烧心和蛀牙的治疗，还可以作为食品补充剂。雷氏链球菌的大规模商业繁殖需要合适的协议。在这里，我们提出了一种改进的协议，用于从结节外植体中繁殖雷氏链球菌。在该协议中，研究了实验室级尿素对来自节点外植体的多次芽诱导的影响。首先将结节外植体在Murashige和Skoog（MS）基础培养基上培养2周，以促进腋芽破裂。此外，将这些外植体在含有6个苄基氨基嘌呤（BAP）（2 mg / L）和萘乙酸（NAA）（1 mg / L）和合成尿素（5 mg / L）的MS培养基上培养一段时间40天的时间显示，在单个节点外植体中，添加尿素的最大枝条产量为44.56，而不含尿素的最大枝条产量为22.44。产生的芽数差异显着，这些芽容易生根于具有NAA（4 mg / L）的MS培养基中。在这些工厂中，一次和二次硬化是成功的。在微繁殖的小苗中没有观察到可见的形态异常。来自随机样本的遗传分析还显示这些植物在遗传上是统一的。本方案的优点是，与现有方案相比，多次芽诱导，生根和硬化的完整过程可以在6个月内完成。

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