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首页> 外文期刊>Theriogenology >Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology
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Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology

机译：使用卵子提取技术影响牛卵体外生产胚胎的卵母细胞恢复和质量的因素

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In Experiment 1, different vacuum pressures (30, 50, 70 and 90 mm Hg) were used to aspirate 4156 bovine follicles in vitro, to assess their effect on flow rate and the recovery, morphology and blastocyst formation of the recovered oocytes. Cumulus oocyte complexes (COCs) were classified according to the morphology of the cumulus cells. Data were analyzed using Chi Square analysis. Overall recovery rate declined as the aspiration pressure increased above 50 nun Hg (P<0.05). The recovery, rate of Grade 1 oocytes decreased significantly (P<0.05) as the vacuum pressure increased with a corresponding increase in the number of denuded oocytes recovered (P<0.05). The blastocyst yield, expressed as a percentage of recovered COCs decreased significantly as the aspiration pressure increased beyond 50 mm Fig (P<0.05). In Experiment 2, the holding media (hepes- or bicarbonate-buffered TCM 199) and holding time (1 h or 5 h) did not affect the blastocyst formation of the oocytes (P>0.05). In Experiment 3, it was found that individual culture of the oocyte during fertilization or culture had a detrimental effect on the 0ocytes blastocyst formation (8.8% to i 6% blastocyst yield on Day 8) when compared to control (31.3%). In Experiment 4, groups of 5, 10 and 25 oocytes were compared with singly cultured oocytes. There were no significant differences (P<0.05) in the blastocyst formation rate among groups of 5, 10, or 25 oocytes, but there was a significant difference between oocytes processed in groups and those processed individually. In Experiment 5, when groups of 10 oocytes were cultured in different drop sizes, there was no significant difference in cleavage rates between oocytes cultured in 100 gL (85.0%, n = 280) and in 10 #mu#L (86.8%, n = 280) of media, but culture in 50 #mu#L (79.3%, n = 280) resulted in cleavage rates sigmficantly lower (P<0.05) than culture in 10 #mu#L drops. There was no significant difference in the blastocyst formation. However there was a sigmficant difference (P<0.05) in cell numbers of Day 8 blastocysts, with oocytes cultured in 1OO#mu#L drops having significantly lower cell counts than oocytes cultured in 50 or 10 #mu#L drops.
机译：在实验1中，使用不同的真空压力（30、50、70和90 mm Hg）在体外抽吸4156个牛卵泡，以评估它们对流速以及回收卵母细胞的回收率，形态和胚泡形成的影响。根据卵丘细胞的形态对卵母细胞复合物（COC）进行分类。使用卡方分析法分析数据。随着抽吸压力增加到高于50尼汞，总体恢复率下降（P <0.05）。 1级卵母细胞的恢复率随着真空压力的增加而显着降低（P <0.05），相应地，裸露卵母细胞的数量也相应增加（P <0.05）。胚泡产量，表示为回收的COC的百分比，随着抽吸压力增加到50 mm以上而显着下降（P <0.05）。在实验2中，保存介质（庚烷或碳酸氢盐缓冲的TCM 199）和保存时间（1 h或5 h）不影响卵母细胞的胚泡形成（P> 0.05）。在实验3中，发现与受精者（31.3％）相比，卵子在受精或培养期间的单独培养对0细胞胚泡形成（第8天胚泡产率为8.8％至6％）具有有害作用。在实验4中，将5个，10个和25个卵母细胞的组与单独培养的卵母细胞进行比较。在5个，10个或25个卵母细胞组中，胚泡形成率没有显着差异（P <0.05），但是在组中处理的卵母细胞与单独处理的卵母细胞之间的胚泡形成率没有显着差异。在实验5中，当以不同的液滴大小培养10个卵母细胞的组时，在100 gL（85.0％，n = 280）和10＃mu＃L（86.8％，n）中培养的卵母细胞之间的裂解率没有显着差异。 = 280）的培养基，但是在50＃mu＃L的培养液中的裂解率（79.3％，n = 280）导致裂解率明显低于10＃mu＃L的培养液的裂解率（P <0.05）。胚泡形成没有明显差异。然而，第8天胚泡的细胞数存在明显差异（P <0.05），其中以100μm＃L液滴培养的卵母细胞比以50或10μμL液滴培养的卵母细胞具有明显更低的细胞计数。

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