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Obtainment of molecular markers linked to BYDV resistance of wheat by RAPD

机译：RAPD获得与小麦BYDV抗性相关的分子标记

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摘要

Wheat yellow dwarf (WYD) caused by the infection of bailey yellow dwarf virus (BYDV) is the main disease of wheat, The yield loss caused by WYD has been estimated at 20%--30%. Three biosystems are associated with WYD: BYDV, vireos-transmitting aphid and wheat host. The creation of BYDV-resistant wheat lines is argued as the most efficient way to control WYD, Though some wheat lines have been reported to show tolerance, there are few, if any, effective sources resisting to BYDV. In contrast, apparentresistances to BYDV have been described in many species of grass, such as Thinopyrum intermedium. Now, the BYDV-resistant genes of Thinopyrum intermedium have been successfully transferred to wheat through wide-cross. Using field-investigation to identify whether the BYDV-resistant genes have been transferred to wheat is a way needing much time and labor. The efficiency will be greatly enhanced if molecular markers linked to these genes are used. This note reports the obtainment of a molecular marker linked to the BYDV resistance genes by randomly amplified polymorphy DNA (RAPD). It is also proved by in situ hybridization that this molecular marker can also be used as a probe specific to X genome of Thinopyrum intermedium.
机译：感染贝利黄矮病毒（BYDV）引起的小麦黄矮（WYD）是小麦的主要病害，由小麦黄矮病毒（WYD）引起的产量损失估计为20％-30％。 WYD与以下三个生物系统相关：BYDV，传播病毒的蚜虫和小麦寄主。建立抗BYDV的小麦系被认为是控制WYD的最有效方法。尽管据报道有些小麦系显示出耐性，但几乎没有（如果有的话）抗BYDV的有效来源。相比之下，在许多草种中，如中间薄Thin（Thinopyrum intermedium），已描述了对BYDV的明显抗性。现在，中间薄线虫的BYDV抗性基因已通过宽杂交成功地转移到小麦中。使用田间调查来确定抗BYDV的基因是否已转移至小麦是一种需要大量时间和精力的方法。如果使用与这些基因连锁的分子标记，效率将大大提高。该说明报道了通过随机扩增的多态性DNA（RAPD）获得与BYDV抗性基因相关的分子标记。通过原位杂交也证明了该分子标记物也可以用作对中间薄壁op草的X基因组特异的探针。

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