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首页> 外文期刊>Vaccine >Interleukin-15 enhance DNA vaccine elicited mucosal and systemic immunity against foot and mouth disease virus.
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Interleukin-15 enhance DNA vaccine elicited mucosal and systemic immunity against foot and mouth disease virus.

机译：白介素15增强DNA疫苗引发针对口蹄疫病毒的粘膜和全身免疫。

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Aerosol transmission of foot and mouth disease virus (FMDV) is believed to be an important route of infection. Induction of mucosal response is thought to be effective way against such infection. Various approaches have been developed including the use of molecules adjuvant and polymers delivery for the mucosal delivery of DNA vaccine. In this study, using low molecular weight chitosan as a delivery vehicle, we investigated whether co-administration intranasally of the FMDV DNA vaccine, pcD-VP1 and a construct expressing IL-15 as the molecular adjuvant can enhance mucosal and systemic immune responses in animals. Compared to the group intranasally immunized with pcD-VP1 alone, the group immunized with the molecular adjuvant not only was induced higher level of mucosal sIgA but also serum IgG. Interestingly, intranasal delivery of the IL-15 construct with pcD-VP1 significantly enhanced the cell-mediated immunity (CMI) compared to the pcD-VP1 alone, as evidenced by the higher level of antigen-specific T-cell proliferation, cytotoxic T lymphocyte (CTL) response and higher expressions of IFN-gamma in both CD4(+) and CD8(+) T cells inform the spleen and mucosal sites. Consistently, IL-15 as adjuvant provided higher level of FMDV neutralizing antibody against FMDV and high secretions of IgA producing cells in mucosal tissues. Taken together, the results demonstrated that intranasal delivery of IL-15 as a mucosal adjuvant can enhance the antigen-specific mucosal and systemic immune responses, which may provide a protection against the FMDV initial infection.
机译：口蹄疫病毒（FMDV）的气溶胶传播被认为是重要的感染途径。粘膜反应的诱导被认为是抵抗这种感染的有效方法。已经开发出各种方法，包括使用分子佐剂和聚合物递送用于DNA疫苗的粘膜递送。在这项研究中，我们以低分子量壳聚糖为载体，研究了FMDV DNA疫苗，pcD-VP1和表达IL-15作为分子佐剂的构建体鼻腔内共同给药能否增强动物的粘膜和全身免疫反应。与单独用pcD-VP1鼻内免疫的组相比，用分子佐剂免疫的组不仅诱导了更高水平的粘膜sIgA，而且诱导了血清IgG。有趣的是，与单独使用pcD-VP1相比，鼻内递送pcD-VP1的IL-15构建体显着增强了细胞介导的免疫（CMI），这可以通过更高水平的抗原特异性T细胞增殖，细胞毒性T淋巴细胞来证明。 （CTL）反应和CD4（+）和CD8（+）T细胞中IFN-γ的更高表达通知了脾脏和粘膜部位。一致地，IL-15作为佐剂可提供更高水平的针对FMDV的FMDV中和抗体和粘膜组织中IgA产生细胞的高分泌。两者合计，结果表明，鼻内递送IL-15作为粘膜佐剂可以增强抗原特异性粘膜和全身免疫应答，这可以提供针对FMDV初始感染的保护。

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