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Attenuation of classical swine fever virus by deletion of the viral N-pro gene

机译：通过删除病毒N-pro基因来减轻经典猪瘟病毒

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We have reported earlier that replacement of the N-pro gene of classical swine fever virus (CSFV) by the murine ubiquitin gene only slightly affects the characteristics of virus replication in the porcine kidney cell line SK-6 [J. Virol. 72 (1998) 768 1]. Here, for the moderately virulent CSFV strain Alfort/187 as well as for the highly virulent strain Eystrup we show that the respective N-pro-deleted viruses are attenuated. Vaccination of pigs with either of the two deletion mutants resulted in the induction of a strong antibody response. Animals were protected against challenge with a lethal dose of highly virulent CSFV indicating that N-pro deletion mutants are excellent candidates for a modified live virus vaccine. A chimeric virus obtained by replacement of the N-pro gene in the Eystrup virus by the corresponding sequence of the avirulent CSFV vaccine strain Riems resulted in a virus that was highly virulent. This indicates that the virulence of CSFV correlates with the presence of N-pro and also suggests that N-pro is not responsible for the varying virulence observed between individual strains of CSFV
机译：早先我们曾报道过用鼠泛素基因替代经典猪瘟病毒（CSFV）的N-pro基因只会轻微影响猪肾细胞系SK-6中病毒复制的特性[J. Chem。Sci。，2002，9，2]。病毒。 72（1998）768 1]。在此，对于中等毒性的CSFV毒株Alfort / 187以及对于高毒力的Eystrup毒株，我们显示了相应的N-pro缺失病毒已被减毒。用两种缺失突变体中的任一种对猪进行疫苗接种可诱导强烈的抗体应答。用致死剂量的高毒性CSFV保护动物免受攻击，表明N-pro缺失突变体是改良活病毒疫苗的优秀候选者。通过用无毒的CSFV疫苗株Riems的相应序列替换Eystrup病毒中的N-pro基因而获得的嵌合病毒产生了高毒力的病毒。这表明CSFV的毒力与N-pro的存在有关，也表明N-pro对各个CSFV毒株之间观察到的毒力变化不承担责任。

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