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首页> 外文期刊>Japanese Journal of Ophthalmology >Histological study on mast cells in conjunctiva of NC/Nga mice
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Histological study on mast cells in conjunctiva of NC/Nga mice

机译：NC / Nga小鼠结膜肥大细胞的组织学研究

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Purpose: To investigate the pathological findings in conjunctiva of NC/Nga mice, which develop atopic dermatitis spontaneously, we focused our study on the density of mast cells as determined by histological methods.Methods: NC/Nga mice were divided into five groups; the 4W-group comprised four 4-week-old mice without treatment, the 10W-group comprised four 10-week-old mice without treatment, the 16W-group comprised four 16-week-old mice without treatment, the Dx group comprised three 16-week-old mice undergoing topical 0.1% dexamethasone (Dx) ointment treatment, and the FK506 group comprised three 16-week-old mice undergoing topical 0.1% FK506 ointment treatment. For the histological examination, the lids and eyeballs of the mice were removed and fixed with Carnoy's solution and thin sections were made. The Carnoy-fixed specimens were stained with toluidine blue or H&E and examined histologically. Toluidine blue-stained tissue sections were examined for the density per square millimeter of mast cells, which were identified as metachromatic cells in the conjunctival tissue and lid cutaneous tissue by light microscopy.Results: Mast cell density in the conjunctiva was 8.6 +/- 8.2 cells/mm(2) in the 4W-group, 29.2 +/- 22.0 cells/mm(2) in the 10W-group, 41.0 +/- 21.1 cells/mm(2) in the 16W-group, 22.7 +/- 17.1 cells/mm(2) in the Dx group, and 33.6 +/- 27.7 cells/mm(2) in the FK506 group. Mast cell density increased significantly with age among the 4W-, 10W-, and 16W-groups (P < 0.001). The mast cell density of lid cutaneous tissue was higher than the conjunctival mast cell density. Mast cell density was significantly higher in the 16W-group than in the Dx group, but not significantly higher than in the FK506 group.Conclusions: An increase in mast cell density with age indicates that NC/Nga mice develop atopic keratoconjunctivitis-like signs. Dexamethasone ointment has a suppressive effect on the increase in mast cell density. (C) Japanese Ophthalmological Society 2004.
机译：目的：探讨自发性发展为特应性皮炎的NC / Nga小鼠结膜的病理学发现，我们将组织学方法用于研究肥大细胞密度的研究。 4W组包括四只未经治疗的4周龄小鼠，10W组包括四只未经治疗的10周龄小鼠，16W组包括四只未经治疗的16周龄小鼠，Dx组包括三只未经治疗的小鼠接受局部0.1％地塞米松（Dx）软膏治疗的16周龄小鼠，FK506组包括三只接受局部0.1％FK506软膏治疗的16周龄小鼠。为了进行组织学检查，将小鼠的眼睑和眼球取下并用卡诺氏溶液固定并制成薄片。 Carnoy固定的标本用甲苯胺蓝或H＆E染色，并进行组织学检查。检查甲苯胺蓝染色的组织切片的肥大细胞每平方毫米的密度，通过光学显微镜将其鉴定为结膜组织和睑皮肤组织中的变色细胞。结果：结膜中的肥大细胞密度为8.6 +/- 8.2 4W组中的电池数量/ mm（2），10W组中的29.2 +/- 22.0电池/ mm（2），16W组中的41.0 +/- 21.1电池/mm(2)、22.7 +/- Dx组中为17.1个/ mm（2），FK506组中为33.6 +/- 27.7个/ mm（2）。在4W，10W和16W组中，肥大细胞密度随年龄显着增加（P <0.001）。眼睑皮肤组织的肥大细胞密度高于结膜肥大细胞密度。结论：16W组肥大细胞密度显着高于Dx组，但不显着高于FK506组。结论：肥大细胞密度随年龄增加表明NC / Nga小鼠发展为特应性角结膜炎样体征。地塞米松软膏对肥大细胞密度的增加具有抑制作用。 （C）日本眼科学会2004。

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