...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Cerebral Blood Flow and Metabolism: Official Journal of the International Society of Cerebral Blood Flow and Metabolism >On the fate of extracellular hemoglobin and heme in brain.
【24h】

On the fate of extracellular hemoglobin and heme in brain.

机译：关于大脑中细胞外血红蛋白和血红素的命运。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Intracerebral hemorrhage (ICH) is a major cause of disability in adults worldwide. The pathophysiology of this syndrome is complex, involving both inflammatory and redox components triggered by the extravasation of blood into the cerebral parenchyma. Hemoglobin, heme, and iron released therein seem be important in the brain damage observed in ICH. However, there is a lack of information concerning hemoglobin traffic and metabolism in brain cells. Here, we investigated the fate of hemoglobin and heme in cultured neurons and astrocytes, as well as in the cortex of adult rats. Hemoglobin was made traceable by conjugation to Alexa 488, whereas a fluorescent heme analogue (tin-protoporphyrin IX) was prepared to allow heme tracking. Using fluorescence microscopy we observed that neurons were more efficient in uptake hemoglobin and heme than astrocytes. Exposure of cortical neurons to hemoglobin or heme resulted in an oxidative stress condition. Viability assays showed that neurons were more susceptible to both hemoglobin and heme toxicity than astrocytes. Together, these results show that neurons, rather than astrocytes, preferentially take up hemoglobin-derived products, indicating that these cells are actively involved in the ICH-associated brain damage.
机译：脑出血（ICH）是全世界成年人残疾的主要原因。该综合征的病理生理学很复杂，涉及由血液渗入脑实质引起的炎症和氧化还原成分。其中释放的血红蛋白，血红素和铁在ICH中观察到的脑损伤中似乎很重要。然而，缺乏关于血红蛋白运输和脑细胞代谢的信息。在这里，我们调查了成年大鼠大脑神经元和星形胶质细胞以及皮质中血红蛋白和血红素的命运。通过缀合到Alexa 488可以追踪血红蛋白，而制备了荧光血红素类似物（锡-原卟啉IX）以进行血红素追踪。使用荧光显微镜，我们观察到神经元比星形胶质细胞更有效地吸收血红蛋白和血红素。皮质神经元暴露于血红蛋白或血红素会导致氧化应激状态。活力测定表明，神经元比星形胶质细胞对血红蛋白和血红素毒性更敏感。总之，这些结果表明，神经元而非星形胶质细胞优先吸收血红蛋白衍生的产物，表明这些细胞积极参与ICH相关的脑损伤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号