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首页> 外文期刊>Journal of Economic Entomology >Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites
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Molecular Diagnostic for Boll Weevil (Coleoptera: Curculionidae) Based on Amplification of Three Species-Specific Microsatellites

机译：基于三种特定物种的微卫星扩增的铃象鼻虫（鞘翅目：Curculionidae）的分子诊断。

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摘要

The boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a serious pest of cultivated cotton, Gossypium hirsutum L., in the Americas, and reinfestation of zones from which they have been eradicated is of perpetual concern. Extensive arrays of pheromone traps monitor for reintroductions, but occasionally the traps collect nontarget weevils that can be misidentified by scouts. For example, the congeneric pepper weevil, Anthonomus eugenii Cano, and other superficially similar weevils are attracted to components of the boll weevil lure or trap color. Although morphologically distinguishable by trained personnel, the potential for misidentification is compounded when captured weevils are dismembered or partially consumed by ants or ground beetles that sometimes feed on them in the traps. Because misidentification can have expensive consequences, a molecular diagnostic tool would be of great value to eradication managers. We demonstrate that a cocktail of three primer pairs in a single polymerase chain reaction (PCR) amplify species-specific microsatellites that unambiguously distinguish the boll weevil from three other weevil species tested, including pepper weevil; cranberry weevil, Anthonomus eugenii musculus Say; and pecan weevil, Curculio caryae Horn. However, it does not distinguish the boll weevil from the subspecific "thurberia" weevil. A universal internal transcribed spacer primer pair included in the cocktail cross-amplifies DNA from all species, serving as a positive control. Furthermore, the diagnostic primers amplified the target microsatellites from various boll weevil adult body parts, indicating that the PCR technology using the primer cocktail is sensitive enough to positively identify a boll weevil even when the body is partly degraded.
机译：象鼻象鼻虫Anthonomus grandis grandis Boheman（鞘翅目：Curculionidae）是在美洲种植的棉花陆地棉（Gossypium hirsutum L.）的一种严重害虫，对其永久根除的地区进行再次侵扰是永久性的问题。大量信息素诱捕器监视重新引入，但有时诱捕器会收集非目标象鼻虫，这些象鼻虫可能会被侦察员误认。例如，同类辣椒象鼻虫，欧氏Anthonomus eugenii Cano和其他表面上类似的象鼻虫被吸引到铃象鼻虫的诱饵或诱集颜色中。尽管可以由受过训练的人员在形态上加以区分，但是当捕获的象鼻虫被有时会在陷阱中觅食的蚂蚁或地面甲虫肢解或部分消耗时，错误识别的可能性会加重。由于误识别可能会带来昂贵的后果，因此分子诊断工具对根除管理人员将具有巨大的价值。我们证明了在单个聚合酶链反应（PCR）中由三个引物对组成的混合物可放大物种特异性微卫星，从而将the象鼻虫与其他三种被测象鼻虫（包括胡椒象鼻虫）毫无区别。蔓越莓象鼻虫（Ethonomus eugenii musculus）说;和山核桃象鼻虫，Curculio caryae Horn。但是，它没有将铃象鼻虫与亚特异的“ thurberia”象鼻虫区分开。鸡尾酒中包含的通用内部转录间隔物引物对可交叉扩增所有物种的DNA，用作阳性对照。此外，诊断性引物从棉铃象鼻虫成年人体的各个部位扩增了目标微卫星，这表明使用引物混合物的PCR技术足够灵敏，即使在身体部分降解的情况下也能正确鉴定棉铃象鼻虫。

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