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首页> 外文期刊>Journal of Economic Entomology >Differential Expression of Candidate Defense Genes of Poplars in Response to Aphid Feeding
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Differential Expression of Candidate Defense Genes of Poplars in Response to Aphid Feeding

机译：杨树候选防御基因对蚜虫摄食的差异表达

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摘要

Species belonging to the genus Populus (poplars) produce a series of defensive proteins in response to insect damage. Proteinase inhibitors, polyphenol oxidases, and chitinases are the most relevant and intensively studied proteins. Most of the knowledge about the relation between these proteins and herbivores has been obtained from studies with chewing insects. Nothing is known about whether phloem-feeder insects such as aphids are able to trigger a comparable response. In the current study, the expression of genes encoding a Kunitz trypsin inhibitor 3 (KTI3), a polyphenol oxidase 1 (PPO1), and a class I chitinase (CHI) was characterized in two poplar hybrids (one resistant hybrid and one susceptible hybrid, to aphids) attacked by the aphid Chaitophorus leucomelas Koch. The expression pattern was analyzed using a semiquantitative reverse transcription-polymerase chain reaction approach. The expression of KTI3 was increased by aphids only in the aphid-susceptible hybrid. Differently, PPO1 expression was increased by aphids in the aphid-resistant hybrid. The expression of CHI was down-regulated by aphids in the susceptible hybrid. This is the first study to report the differential expression of poplar defense genes in response to phloem-feeder insects such as aphids. The findings from the current study suggest that the expression levels of defensive proteins are affected by poplar genotype and by aphid infestation.
机译：属于胡杨属（杨树）的物种产生一系列防御蛋白质，以应对昆虫的伤害。蛋白酶抑制剂，多酚氧化酶和几丁质酶是最相关和深入研究的蛋白。这些蛋白质与食草动物之间关系的大多数知识是从对咀嚼昆虫的研究中获得的。韧皮部食虫昆虫（如蚜虫）是否能够触发类似反应还一无所知。在目前的研究中，在两个杨树杂种中（一个抗性杂种和一个易感杂种，蚜虫Chaitophorus leucomelas Koch攻击。使用半定量逆转录-聚合酶链反应方法分析表达模式。蚜虫仅在易感蚜虫的杂种中增加了KTI3的表达。不同的是，在抗蚜虫的杂种中，蚜虫增加了PPO1的表达。 CHI的表达被易感杂种中的蚜虫下调。这是第一个报道杨树防御基因对蚜虫等韧皮部食虫昆虫反应的差异表达的研究。当前研究的发现表明防御蛋白​​的表达水平受杨基因型和蚜虫侵染的影响。

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