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首页> 外文期刊>Journal of Food Safety >EFFECT OF HIGH-INTENSITY PULSED ELECTRIC FIELDS ON SURVIVAL OF ESCHERICHIA COLI K-12 SUSPENDED IN MEAT INJECTION SOLUTIONS
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EFFECT OF HIGH-INTENSITY PULSED ELECTRIC FIELDS ON SURVIVAL OF ESCHERICHIA COLI K-12 SUSPENDED IN MEAT INJECTION SOLUTIONS

机译：高强度脉冲电场对肉类注射液中悬浮的大肠杆菌K-12存活的影响

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摘要

Survival of Escherichia coli K12 suspended in solutions used for enhancing meat products after high-intensity pulsed electric field (PEF) treatments was examined. Solutions were prepared to provide 0.3% salt, 0.3% phosphate and-or 0, 1, 2 or 3% sodium lactate in a finished product enhanced to 110% of initial weight. Therefore, enhancement solutions contained water, 3% NaCl, 3% phosphate and-or 0, 10, 20 or 30% sodium lactate. Samples containing 0.1% NaCl were run as controls. Single PEF strengths used for PEF treatments were 12.5, 7.0, 6.0, 5.0 and 3.5 kV-cm. For 12.5, 7.0 and 6.0 kV-cm, two levels of pulse controller resistance were used, proportional to and 200 ohm; for 5 and 3.5 kV-cm, only 200 ohm was tested. Above 7 kV-cm, arcing occurred which limited the application of this technology in these solutions. Electrical field strength at 7 kV-cm with a pulse controller resistance of 200 ohm resulted in a reduction of about 2 log cfu-mL when cells were suspended in the enhancement solution containing no lactate. Lower electrical field strengths ( greater than or equal to 6 kV-cm) were generally ineffective.
机译：检查了高强度脉冲电场（PEF）处理后悬浮在用于增强肉制品的溶液中的大肠杆菌K12的存活率。制备溶液以提供增加至初始重量的110％的最终产品中的0.3％盐，0.3％磷酸盐和-或0、1、2或3％乳酸钠。因此，增强溶液包含水，3％NaCl，3％磷酸盐和-或0、10、20或30％的乳酸钠。将含有0.1％NaCl的样品作为对照。用于PEF处理的单个PEF强度为12.5、7.0、6.0、5.0和3.5 kV-cm。对于12.5、7.0和6.0 kV-cm，使用了两个级别的脉冲控制器电阻，分别与200 ohm和200 ohm成正比。对于5和3.5kV-cm，仅测试了200欧姆。高于7 kV-cm时，发生电弧放电，从而限制了该技术在这些解决方案中的应用。当细胞悬浮在不含乳酸的增强溶液中时，在7 kV-cm的电场强度和200 ohm的脉冲控制器电阻会导致大约2 log cfu-mL的降低。较低的电场强度（大于或等于6 kV-cm）通常无效。

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