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首页> 外文期刊>Journal of Food Lipids >Modification of fish oil by lipozyme TL IM to produce structured lipid
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Modification of fish oil by lipozyme TL IM to produce structured lipid

机译：通过脂酶TL IM修饰鱼油以产生结构化脂质

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摘要

Stearic acid methyl esters was enzymatically interesterified with fish oil (EPAX 5500) containing 44.5 and 32.6 mol% eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3), respectively. Lipozyme TL IM from Thermomyces lanuginosus was used to produce structured lipids (SL) that may be suitable for margarine or shortening application. Interesterification was performed in hexane. Fish oil: stearic acid methyl ester levels ranging from 1 to 5 mole ratio was used. The effect of incubation time, substrate ratio and incubation temperature were also studied. Generally, as incubation time and substrate ratio increased, so did the mol% incorporation of stearic acid. After 24 h incubation in hexane, there was a 49.4 mol% incorporation of stearic acid into fish oil, while the mol% of EPA and DHA were reduced to 15.0 and 13.0 mol%, respectively. Time course studies also indicated that the highest stearic acid incorporation occurred at 72 h while 1:5 fish oil to stearic acid mole ratio gave the highest stearic acid incorporation. The data suggests that Lipozyme TL IM could be used to produce SL.
机译：分别用含有44.5和32.6 mol％二十碳五烯酸（EPA，C20：5n-3）和二十二碳六烯酸（DHA，C22：6n-3）的鱼油（EPAX 5500）对硬脂酸甲酯进行酶促酯交换。来自羊毛嗜热霉菌的Lipozyme TL IM被用于生产结构化脂质（SL），该脂质可能适用于人造黄油或起酥油的应用。在己烷中进行酯交换。鱼油：硬脂酸甲酯的用量为1-5摩尔比。还研究了孵育时间，底物比例和孵育温度的影响。通常，随着温育时间和底物比率的增加，硬脂酸的摩尔百分比掺入也增加。在己烷中孵育24小时后，硬脂酸掺入到鱼油中的比例为49.4 mol％，而EPA和DHA的mol％分别降低至15.0和13.0 mol％。时程研究还表明，最高的硬脂酸掺入发生在72小时，而1：5的鱼油与硬脂酸的摩尔比则表明最高的硬脂酸掺入。数据表明，Lipozyme TL IM可用于生产SL。

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