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A simple and highly sensitive elisa for screening of the α-thalassemia-1 southeast asian-type deletion

机译:一种简单且高度敏感的酶联免疫吸附法,用于筛选α-地中海贫血-1东南亚型缺失

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摘要

Couples in which both partners carry the α-thalassemia-1 trait have a 25% risk of hemoglobin Bart's hydrops fetalis in each pregnancy. Identification of α-thalassemia-1 trait is, therefore, necessary in order to control this severe form of α-thalassemia. We have generated monoclonal antibodies specific to the ζ-globin chain without cross reaction with other globin chains. A simple and sensitive ELISA was developed by using poly-l-lysine to increase the protein binding to the ELISA plate. The developed poly-l-lysine pre-coated ELISA has a very high sensitivity (100%) and specificity (97%) for detection of carriers of α-thalassemia-1 with Southeast Asian-type deletion.
机译:夫妻双方都具有α-地中海贫血1特质的夫妇在每次怀孕中都有25%的血红蛋白巴特氏胎儿积水风险。因此,为了控制这种严重形式的α-地中海贫血,必须鉴定α-地中海贫血-1的性状。我们已经生成了对ζ珠蛋白链具有特异性的单克隆抗体,而没有与其他珠蛋白链发生交叉反应。通过使用聚-1-赖氨酸来增加蛋白质与ELISA板的结合,开发了一种简单而灵敏的ELISA。所开发的聚-1-赖氨酸预涂ELISA具有很高的灵敏度(100%)和特异性(97%),可用于检测带有东南亚型缺失的α-地中海贫血-1的携带者。

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