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首页> 外文期刊>Journal of industrial and engineering chemistry >Split addition of enzymes in enzymatic hydrolysis at high solids concentration to increase sugar concentration for bioethanol production
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Split addition of enzymes in enzymatic hydrolysis at high solids concentration to increase sugar concentration for bioethanol production

机译：在高固体浓度的酶解过程中分批添加酶，以增加糖浓度以生产生物乙醇

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摘要

One challenge in making bioethanol production economical is to increase total solids in hydrolysis system while maintaining sugar conversion efficiency. Because the removal of excess water from hydrolysate requires enormous amounts of heat, large volume of reaction towers and high capital expenditure (CAPEX) for equipment, a lengthy operating time, and high operating costs. When solids loading in hydrolysis system increased from 5% to 20% with no mixing strategies, final sugar conversion decreased markedly. If cellulase is mixed with pulp at 5% solids and pressed to 20% solids, then 80% of the cellulase retained in the pulp thinned down the pulp mixture in 2 h. This thinning effect enabled additional cellulase, xylanase, and β-glucosidase to be mixed into the slurry. Sugar concentration was significantly improved; from 26 g/L to 121 g/L, while sugar conversion was remained as enzymatic hydrolysis with 5% total solids enzymatic hydrolysis. A US patent has been granted to NCSU for this concept and licenses have been granted to various companies.
机译：使生物乙醇生产经济的一个挑战是增加水解系统中的总固体，同时保持糖的转化效率。因为从水解产物中除去过量的水需要大量的热量，大量的反应塔和设备的高资本支出（CAPEX），较长的运行时间以及较高的运行成本。如果在没有混合策略的情况下，水解系统中的固体含量从5％增加到20％，则最终的糖转化率会明显下降。如果将纤维素酶与纸浆以5％的固体含量混合并压成20％的固体含量，则保留在纸浆中的80％的纤维素酶会在2小时内使纸浆混合物变稀。这种稀化作用使额外的纤维素酶，木聚糖酶和β-葡萄糖苷酶可以混合到浆液中。糖浓度明显改善;从26g / L至121g / L，而糖转化率保持为酶水解，总固体酶水解为5％。 NCSU已为此概念授予美国专利，并已向多家公司授予许可。

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