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首页> 外文期刊>Journal of Leukocyte Biology: An Official Publication of the Reticuloendothelial Society >Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.
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Enrichment for a CD26hi SIRP- subset in lymph dendritic cells from the upper aero-digestive tract.

机译：来自上消化道的淋巴树突状细胞中CD26hi SIRP-亚型的富集。

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Dendritic cells (DC) have been reported to migrate in afferent lymph in the steady state. However, it is unknown whether DC traffic is modulated by the nature of the drained tissue. To analyze the influence of mucosal versus cutaneous microenvironments on the constitutive DC release, we exploited a novel technique of lymph cannulation in sheep, which allowed a comparison of afferent lymph DC migrating from the head mucosae [cervical DC (CerDC)] with DC migrating from skin [prescapular DC (PresDC)]. The migration rate was lower for CerDC than for PresDC. Compared with PresDC, CerDC contained a higher proportion of the CD26hi signal regulatory protein (SIRP)- DC subset. It is interesting that cytoplasmic apoptotic DNA as well as cytokeratin-positive inclusions were primarily detected among CD26hi SIRP- DC, an observation similar to that made in rats, which leads to the suggestion that this subset was involved in self-antigen presentation and tolerance induction. After the inoculation of cholera toxin (CT) onto the oro-nasal mucosae, migration of CD26hi SIRP- and CD26lo SIRP+ DC was accelerated in lymph, indicating that the effect of CT on DC mobilization is not subset-specific. Our results show that a mucosal environment influences DC output and the relative DC subset representation in lymph. This modulation of DC traffic to lymph nodes by mucosal surfaces is likely to affect the bias of the mucosal immune responses.
机译：据报道，树突状细胞（DC）在稳定状态下会在传入淋巴中迁移。然而，DC引流是否受引流组织的性质调节尚不清楚。为了分析粘膜和皮肤微环境对本构性DC释放的影响，我们开发了绵羊淋巴插管的一种新技术，该技术可以比较从头部粘膜[子宫颈DC（CerDC）]迁移的传入淋巴DC与从头状粘膜迁移的DC皮肤[肩cap前DC（PresDC）]。 CerDC的迁移率低于PresDC。与PresDC相比，CerDC包含更高比例的CD26hi信号调节蛋白（SIRP）-DC子集。有趣的是，在CD26hi SIRP-DC中主要检测到细胞质凋亡DNA以及细胞角蛋白阳性包涵体，这一观察结果与大鼠相似，从而提示该亚群参与了自身抗原的呈递和耐受诱导。 。在口鼻粘膜上接种霍乱毒素（CT）后，CD26hi SIRP-和CD26lo SIRP + DC在淋巴中的迁移加速，这表明CT对DC动员的影响不是亚群特异性的。我们的结果表明，粘膜环境会影响DC输出和淋巴中的相对DC子集表示。粘膜表面对淋巴结的DC流量的这种调制可能会影响粘膜免疫反应的偏倚。

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