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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Proton dependence of tobacco mosaic virus dissociation by pressure
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Proton dependence of tobacco mosaic virus dissociation by pressure

机译：压力引起的烟草花叶病毒解离的质子依赖性

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Tobacco mosaic virus (TMV) is an intensely studied model of viruses. This paper reports an investigation into the dissociation of TMV by pH and pressure up to 220 MPa. The viral solution (0.25 mg/ml) incubated at 277 K showed a significant decrease in light scattering with increasing pH, suggesting dissociation. This observation was confirmed by HPLC gel filtration and electron microscopy. The calculated volume change of dissociation (AV) decreased (absolute value) from - 49.7 ml/mol of subunit at pH 3.8 to - 21.7 ml/mol of subunit at pH 9.0. The decrease from pH 9.0 to 3.8 caused a stabilization of 14.1 kJ/mol of TMV subunit. The estimated proton release calculated from pressure-induced dissociation curves was 0.584 mol H+/mol of TMV subunit. These results suggest that the degree of virus inactivation by pressure and the immunogenicity of the inactivated structures can be optimized by modulating the surrounding pH, (C) 2004 Elsevier B.V. All rights reserved.
机译：烟草花叶病毒（TMV）是一种经过深入研究的病毒模型。本文报道了通过pH和高达220 MPa的压力使TMV分解的研究。在277 K下温育的病毒溶液（0.25 mg / ml）随着pH的增加，光散射显着降低，这表明其解离。该观察结果通过HPLC凝胶过滤和电子显微镜确认。计算出的解离体积变化（AV）（绝对值）从pH 3.8时的-49.7 ml / mol的亚基降至pH 9.0时的-21.7 ml / mol的亚基。从pH 9.0降至3.8导致TMV亚基稳定14.1 kJ / mol。根据压力诱导的解离曲线计算出的估计质子释放为0.584 mol H + / mol TMV亚基。这些结果表明可以通过调节周围的pH来优化通过压力使病毒失活的程度和失活结构的免疫原性，（C）2004 Elsevier B.V.保留所有权利。

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