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首页> 外文期刊>Journal of Neurophysiology >Postnatal development of electrophysiological properties of nucleus accumbens neurons.
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Postnatal development of electrophysiological properties of nucleus accumbens neurons.

机译：伏伏核神经元的电生理特性的产后发育。

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摘要

We have studied the postnatal development of the physiological characteristics of nucleus accumbens (nAcb) neurons in slices from postnatal day 1 (P1) to P49 rats using the whole cell patch-clamp technique. The majority of neurons (102/108) were physiologically identified as medium spiny (MS) projection neurons, and only these were subjected to detailed analysis. The remaining neurons displayed characteristics suggesting that they were not MS neurons. Around the time of birth and during the first postnatal weeks, the membrane and firing characteristics of MS neurons were quite different from those observed later. These characteristics changed rapidly during the first 3 postnatal weeks, at which point they began to resemble those found in adults. Both whole cell membrane resistance and membrane time constant decreased more than fourfold during the period studied. The resting membrane potential (RMP) also changed significantly from an average of -50 mV around birth to less than -80 mV by the end of the third postnatal week. During the first postnatal week, the current-voltage relationship of all encountered MS neurons was linear over a wide range of membrane potentials above and below RMP. Through the second postnatal week, the proportion of neurons displaying inward rectification in the hyperpolarized range increased steadily and after P15, all recorded MS neurons displayed significant inward rectification. At all ages, inward rectification was blocked by extracellular cesium and tetra-ethyl ammonium and was not changed by 4-aminopyridine; this shows that inward rectification was mediated by the same currents in young and mature MS neurons. MS neurons fired single and repetitive Na(+)/K(+) action potentials as early as P1. Spike threshold and amplitude remained constant throughout development in contrast to spike duration, which decreased significantly over the same period. Depolarizing current pulses from rest showed that immature MS neurons fired action potentials more easily than their older counterparts. Taken together, the results from the present study suggest that young and adult nAcb MS neurons integrate excitatory synaptic inputs differently because of differences in their membrane and firing properties. These findings provide important insights into signal processing within nAcb during this critical period of development.
机译：我们使用全细胞膜片钳技术研究了从出生后第1天（P1）到P49大鼠切片中伏伏核（nAcb）神经元生理特征的产后发育。在生理上，大多数神经元（102/108）被识别为中等棘突（MS）投射神经元，仅对这些神经元进行了详细分析。其余的神经元显示出特征，表明它们不是MS神经元。大约在出生时和出生后的头几周，MS神经元的膜和放电特性与后来观察到的完全不同。在出生后的前三个星期中，这些特征迅速改变，从那时开始，它们开始类似于成年人的特征。在研究期间，全细胞膜电阻和膜时间常数均下降了四倍以上。产后第三周末，静息膜电位（RMP）也从出生时的平均-50 mV显着变化到低于-80 mV。在产后的第一个星期内，所有遇到的MS神经元的电流-电压关系在RMP之上和之下的大范围膜电位上都是线性的。在产后第二周，在超极化范围内显示内向矫正的神经元比例稳定增加，在P15之后，所有记录的MS神经元均显示出明显的内向矫正。在所有年龄段，内向整流均被胞外铯和四乙基铵阻止，而4-氨基吡啶则不会改变。这表明内向整流是由年轻和成熟的MS神经元中的相同电流介导的。 MS神经元最早在P1发射单个和重复的Na（+）/ K（+）动作电位。与峰值持续时间相反，峰值阈值和振幅在整个开发过程中保持恒定，峰值持续时间在同一时期显着下降。休息时对电流脉冲进行去极化显示，未成熟的MS神经元比其较老的神经元更容易激发动作电位。两者合计，本研究的结果表明，年轻的和成年的nAcb MS神经元整合的兴奋性突触输入不同，因为它们的膜和发射特性不同。这些发现为开发关键阶段的nAcb内的信号处理提供了重要的见识。

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